scholarly journals Identification of RAPD Markers and Development of SCAR Markers Linked to a Powdery Mildew Resistance Gene, and their Location on Chromosome in Wheat Cultivar Brock

2005 ◽  
Vol 8 (5) ◽  
pp. 578-585 ◽  
Author(s):  
Zhenying Wang ◽  
Pei Zhao ◽  
Hong Chen ◽  
Youngkang Peng ◽  
Chaojie Xie ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Rapd Markers ◽  
Powdery Mildew Resistance ◽  
Wheat Cultivar ◽  
Scar Markers ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Identification of coupling and repulsion phase RAPD markers for powdery mildew resistance gene er-1 in pea

Genome ◽  
1998 ◽  
Vol 41 (3) ◽  
pp. 440-444 ◽  
Author(s):  
K R Tiwari ◽  
G A Penner ◽  
T D Warkentin
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Rapd Markers ◽  
Powdery Mildew Resistance ◽  
Bulked Segregant Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Powdery Mildew Resistance Gene ◽  
Erysiphe Pisi ◽  
Mildew Resistance ◽  
Repulsion Phase

Powdery mildew is a serious disease of pea caused by the obligate parasite Erysiphe pisi Syd. Random amplified polymorphic DNA (RAPD) analysis has emerged as a cost-effective and efficient marker system. The objective of this study was to identify RAPD markers for powdery mildew resistance gene er-1. The resistant cultivar Highlight (carrying er-1) and the susceptible cultivar Radley were crossed, and F3 plants were screened with Operon (OP) and University of British Columbia (UBC) primers, using bulked segregant analysis. A total of 416 primers were screened, of which amplicons of three Operon primers, OPO-18, OPE-16, and OPL-6, were found to be linked to er-1. OPO-181200 was linked in coupling (trans to er-1) and no recombinants were found. OPE-161600 (4 ± 2 cM) and OPL-61900 (2 ± 2 cM) were linked in repulsion (cis to er-1). The fragments OPO-181200 and OPE-161600 were sequenced and specific primers designed. The specific primer pair Sc-OPO-181200 will be useful in identifying homozygous resistant individuals in F2 and subsequent segregating generations. Sc-OPE-161600 will have greatest utility in selecting heterozygous BC\dn6 nF1 individuals in backcross breeding programs.Key words: bulked segregant analysis,Erysiphe pisi, pea, RAPD.

Molecular Mapping and Chromosomal Location of Powdery Mildew Resistance Gene in Wheat Cultivar Tangmai 4

2008 ◽  
Vol 34 (7) ◽  
pp. 1193-1198
Author(s):  
Tie-Zhu HU ◽  
Hong-Jie LI ◽  
Chao-Jie XIE ◽  
Ming-Shan YOU ◽  
Zuo-Min YANG ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Chromosomal Location ◽  
Molecular Mapping ◽  
Wheat Cultivar ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Molecular Mapping of Powdery Mildew Resistance Gene in Wheat Cultivar Jimai 22

2009 ◽  
Vol 35 (8) ◽  
pp. 1425-1431 ◽  
Author(s):  
Gui-Hong YIN ◽  
Gen-Ying LI ◽  
Zhong-Hu HE ◽  
Jian-Jun LIU ◽  
Hui WANG ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Molecular Mapping ◽  
Wheat Cultivar ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance

Molecular mapping of a recessive powdery mildew resistance gene in spelt wheat cultivar Hubel

2014 ◽  
Vol 34 (2) ◽  
pp. 491-500 ◽  
Author(s):  
Fuxiang Peng ◽  
Na Song ◽  
Hongxia Shen ◽  
Haibin Wu ◽  
Hongtu Dong ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Molecular Mapping ◽  
Wheat Cultivar ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Spelt Wheat

scholarly journals Detection of Powdery Mildew Resistance Gene in Melon Cultivar Meloni Based on SCAR Markers

2020 ◽  
Vol 12 (1) ◽  
pp. 76-82
Author(s):  
Muhammad Alif Ishak ◽  
Budi Setiadi Daryono
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Powdery Mildew Resistance ◽  
Fungal Infections ◽  
Plant Diseases ◽  
Scar Markers ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
Sequence Characterized Amplified Region ◽  
Melon Seeds

Powdery mildew is one of the diseases caused by fungal infections that can reduce the production of melon fruit worldwide including in Indonesia. A powdery mildew-resistant cultivar of melon is needed to increase melon yield crops. This study aimed to detect resistance gene linked to powdery mildew using a sequence characterized amplified region (SCAR) markers. The melon cultivar Meloni was used in this study. SL-3, PI 371795, and Aramis cultivar were used to compare. Amplification of the marker was performed employing a pair of primers. The result showed that Meloni had a powdery mildew resistance gene by the presence of a DNA target band at 1058 base pair (bp). Based on this result, it could be concluded that Meloni was an excellent melon cultivar because of its ability to overcome the powdery mildew infections naturally. SCAR markers have been used for various purposes, especially to detect resistance genes to plant diseases. The present study had provided information for plant breeders about Meloni as the new melon cultivar that was genetically resistant against powdery mildew infections. Furthermore, Meloni could be proposed as an alternative to native Indonesian superior melon seeds.

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