scholarly journals Improvement of Tolerance to Heat Stress Given during Hot Water Disinfection of Rice Seeds by Reducing their Water Contents

2013 ◽  
Vol 82 (4) ◽  
pp. 397-401
Author(s):  
Motoki Kanekatsu ◽  
Yoshiki Mitamura ◽  
Naoto Okazaki ◽  
Naoto Sano ◽  
Tetsuya Yamada ◽  
...  
2017 ◽  
Vol 86 (2) ◽  
pp. 177-185
Author(s):  
Megumi Kashiwagi ◽  
Kazumasa Murata ◽  
Hadian Permana ◽  
Tetsuya Yamada ◽  
Motoki Kanekatsu

2017 ◽  
Vol 16 (4) ◽  
pp. 211-220 ◽  
Author(s):  
Hadian Permana ◽  
Kazumasa Murata ◽  
Megumi Kashiwagi ◽  
Tetsuya Yamada ◽  
Motoki Kanekatsu

ASAIO Journal ◽  
2012 ◽  
pp. 1 ◽  
Author(s):  
Tomonari Ogawa ◽  
Akihiko Matsuda ◽  
Yumiko Yamaguchi ◽  
Yusuke Sasaki ◽  
Yuki Kanayama ◽  
...  

2020 ◽  
Vol 2020 ◽  
pp. 1-11
Author(s):  
Ying Hou ◽  
Peipei Yuan ◽  
Yang Fu ◽  
Qi Zhang ◽  
Yaxin Wei ◽  
...  

Background. Duzhong Butiansu (DZBTS) prescription contains many traditional Chinese medicines and has been shown to have a curative effect on male fertility. However, the efficacy and mechanism of DZBTS in the treatment of male infertility induced by heat stress have not been reported. The aim of the present study is to elucidate the effect and mechanism of DZBTS on spermatogenic function of a heat stress model in rats. Methods. Male Wistar rats (280–320 g) were given different doses of DZBTS (0.4853 g/kg/d or 0.9707 g/kg/d), Shengjing capsule (0.56 g/kg/d), or double distilled water for 15 days. A 43°C hot water bath for 30 minutes was used to stimulate the testis of rats. Sperm count, sperm motility, the organ index of kidney and gonadal organs, serum sex hormone levels, and serum oxidising reaction index were measured. Haematoxylin and eosin (HE) staining was used to observe the morphology of the testis and kidney. The expression of Hsp70 in testes was observed by immunofluorescence. The changes in heat stress, reproductive-related protein, and mRNA were measured by western blot assay and RT-qPCR. Results. Heat stress downregulated the levels of sex hormone (P<0.05 or P<0.01) and its receptor androgen receptor (AR) protein expression and mRNA (P<0.01) in rats. Meanwhile, heat stress downregulated the levels of CAMP-responsive element-binding (CREB1) protein and mRNA (P<0.01), which are involved with spermatogenesis. Heat stress also decreased the oxidative damage index. Furthermore, Hsp70 and the heat shock transcription factor 1 (HSF1) protein pathway and mRNA level were overactivated (P<0.05 or P<0.01). Finally, the organ coefficients of the kidney and gonadal organs of rats were decreased. The sperm concentration and motility also decreased significantly (P<0.01). DZBTS could recover these changes induced by heat stress. Conclusions. Our results for the first time have found that DZBTS can improve spermatogenesis disorder in a heat stress model in rats, which may be mainly by regulating AR, sperm regulatory protein CREB1, and the HSF/Hsp70 signaling pathway to decrease oxidative stress.


2020 ◽  
Vol 15 (4) ◽  
pp. 1934578X2091728
Author(s):  
Yoshihiko Nishioka ◽  
Seiya Nishikawa ◽  
Toshiyuki Shibata

Sideritis scardica is a Lamiaceae plant that is endemic to the alpine zone of the Balkan Peninsula. The tea of S. scardica has been handed down as a “tea of longevity” in the Rhodope region of Bulgaria for an unknown amount of time. In this study, we prepared a hot water extract of S. scardica (SHWE) and examined its effects on both life span and stress response in living tissue using Caenorhabditis elegans and its transgenic mutants. The life span of wild-type N2 worms was prolonged by approximately 15% at the SHWE concentration of 5 µg/mL and approximately 22% at the SHWE concentration of 50 µg/mL, as compared with the control group. The effect of SHWE on the expression of heat shock protein 16.2 (HSP-16.2) under heat stress was investigated using TJ375 worms, a transgenic mutant of C. elegans. In the TJ375 worms pretreated with SHWE, the fluorescence intensity of green fluorescent protein fluorescence, which indicates the expression of HSP-16.2, was significantly increased. In the assay using TJ356 worms, the worms pretreated with SHWE did not show the translocation of DAF-16, a forkhead transcription factor class O homolog, from the cytoplasm to nucleus under heat stress. Additionally, under heat stress, the pretreatment of SHWE improved the survival rate of GR1307 worms, a knockout mutant of daf-16. These results indicate that SHWE enhances HSP-16.2 expression through a stress-response pathway (eg, HSF-1 pathway) other than the DAF-16 pathway, resulting in a prolonged life span of C. elegans under heat stress.


2014 ◽  
Vol 94 ◽  
pp. 66-73 ◽  
Author(s):  
Alice Spadoni ◽  
Michela Guidarelli ◽  
Simona Marianna Sanzani ◽  
Antonio Ippolito ◽  
Marta Mari

2020 ◽  
Vol 10 (2) ◽  
pp. 141-146
Author(s):  
Irda Safni ◽  
Uci Utari ◽  
Maryani Cyccu Tobing ◽  
Lisnawita Lisnawita

Burkholderia glumae is a seed-borne pathogen of rice known to cause bacterial panicle blight disease. The lack of effective control methods makes seed treatment the alternative management approach. The aim of this research was to determine an effective seed treatments technique, using liquid smoke, clove oil, hot water and copper hydroxide fungicide treatment against bacteria B. glumae. The experiment used a complete randomized design with five treatments and three replications, including control, liquid smoke, clove oil, hot water, and copper hydroxide fungicide. The results showed the propensity for all treatments to reduce bacterial populations on rice seeds, while liquid smoke, clove oil, and fungicide did not reduce vigour and viability. Application of copper hydroxide fungicide 77% at concentration of 5% was recommended as the best treatment to control the bacterial pathogen. Keywords: Burkholderia glumae, clove oil, copper hydroxide fungicide, hot water, liquid smoke, rice


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