scholarly journals Studies on the Stomatal Movement of Tobacco Leaves : The behavior of stomata in the upper and lower surfaces of intact tobcco leaves in relation to transpiration and photosynthesis

1974 ◽  
Vol 43 (4) ◽  
pp. 510-516 ◽  
Author(s):  
Mitsuyoshi SHODA
Author(s):  
S. Edith Taylor ◽  
Patrick Echlin ◽  
May McKoon ◽  
Thomas L. Hayes

Low temperature x-ray microanalysis (LTXM) of solid biological materials has been documented for Lemna minor L. root tips. This discussion will be limited to a demonstration of LTXM for measuring relative elemental distributions of P,S,Cl and K species within whole cells of tobacco leaves.Mature Wisconsin-38 tobacco was grown in the greenhouse at the University of California, Berkeley and picked daily from the mid-stalk position (leaf #9). The tissue was excised from the right of the mid rib and rapidly frozen in liquid nitrogen slush. It was then placed into an Amray biochamber and maintained at 103K. Fracture faces of the tissue were prepared and carbon-coated in the biochamber. The prepared sample was transferred from the biochamber to the Amray 1000A SEM equipped with a cold stage to maintain low temperatures at 103K. Analyses were performed using a tungsten source with accelerating voltages of 17.5 to 20 KV and beam currents from 1-2nA.


2009 ◽  
Vol 35 (8) ◽  
pp. 1491-1499 ◽  
Author(s):  
Lin ZHANG ◽  
Xiang ZHAO ◽  
Ya-Jing WANG ◽  
Xiao ZHANG

2013 ◽  
Vol 38 (2) ◽  
pp. 374-379 ◽  
Author(s):  
Zhi-Li PAN ◽  
Meng QI ◽  
Chun-Yang WEI ◽  
Feng LI ◽  
Shi-Xiang ZHANG ◽  
...  

2013 ◽  
Vol 31 (3) ◽  
pp. 278
Author(s):  
Wen-Qi XIE ◽  
Jin-Ping ZHANG ◽  
Jian-Yi TAN ◽  
Xiao-Li XUAN ◽  
Yong-Fei WANG ◽  
...  

2018 ◽  
Author(s):  
Benedict M. Long ◽  
Wei Yih Hee ◽  
Robert E. Sharwood ◽  
Benjamin D. Rae ◽  
Sarah Kaines ◽  
...  
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