Analysis of the SIX2, SIX1 and P300 cistromes in 16-17 week old human fetal kidney cortex

2019 ◽  
Author(s):  
L O'Brien
Keyword(s):  
Kidney Cortex ◽  
Fetal Kidney ◽  
Human Fetal Kidney

scholarly journals Gαs Transcripts Are Biallelically Expressed in the Human Kidney Cortex: Implications for Pseudohypoparathyroidism Type 1b

2001 ◽  
Vol 86 (10) ◽  
pp. 4627-4629 ◽  
Author(s):  
Hong Zheng ◽  
Genia Radeva ◽  
Jennifer A. McCann ◽  
Geoffrey N. Hendy ◽  
Cynthia G. Goodyer
Keyword(s):  
Human Kidney ◽  
Proximal Tubules ◽  
Kidney Cortex ◽  
Rt Pcr ◽  
Fetal Kidney ◽  
Specific Expression ◽  
Renal Proximal Tubules ◽  
Maternal Mrnas ◽  
Pcr Assays ◽  
Human Fetal Kidney

Pseudohypoparathyroid type 1b patients are characterized by renal resistance to PTH in the absence of Albright’s hereditary osteodystrophy or other endocrine abnormalities. Kindred studies have suggested that the cause of this resistance is a specific decrease in Gαs activity in renal proximal tubules due to paternal imprinting of Gαs. To test this, allelic expression of Gαs was analyzed in human fetal kidney cortex samples by RT-PCR assays. The results showed that, in contrast to the parent-specific expression of exon 1A and XLαs (paternal) or NESP (maternal) mRNAs, Gαs transcripts are biallelically expressed in human kidney cortex. These data implicate abnormal imprinting of alternative regions within the GNAS1 locus as a more likely cause of pseudohypoparathyroid type 1b.

scholarly journals Possible Relevance of Soluble Luteinizing Hormone Receptor during Development and Adulthood in Boys and Men

Cancers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1329
Author(s):  
Li Juel Mortensen ◽  
Mette Lorenzen ◽  
Anne Jørgensen ◽  
Jakob Albrethsen ◽  
Niels Jørgensen ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Hormone Receptor ◽  
Adrenal Glands ◽  
Seminal Fluid ◽  
Body Fluids ◽  
Luteinizing Hormone Receptor ◽  
Gonadal Function ◽  
Fetal Kidney ◽  
Healthy Men ◽  
Human Fetal Kidney

Luteinizing hormone (LH) and human chorionic gonadotropin (hCG) are agonists for the luteinizing hormone receptor (LHCGR) which regulates male reproductive function. LHCGR may be released into body fluids. We wish to determine whether soluble LHCGR is a marker for gonadal function. Cross-sectional, longitudinal, and intervention studies on 195 healthy boys and men and 396 men with infertility, anorchia, or Klinefelter Syndrome (KS) were used to correlate LHCGR measured in serum, seminal fluid, urine, and hepatic/renal artery and vein with gonadal function. LHCGR was determined in fluids from in vitro and in vivo models of human testicular tissue and cell lines, xenograft mouse models, and human fetal kidney and adrenal glands. Western blot showed LHCGR fragments in serum and gonadal tissue of similar size using three different antibodies. The LHCGR-ELISA had no species cross-reactivity or unspecific reaction in mouse serum even after human xenografting. Instead, sLHCGR was released into the media after the culture of a human fetal kidney and adrenal glands. Serum sLHCGR decreased markedly during puberty in healthy boys (p = 0.0001). In healthy men, serum sLHCGR was inversely associated with the Inhibin B/FSH ratio (β −0.004, p = 0.027). In infertile men, seminal fluid sLHCGR was inversely associated with serum FSH (β 0.006, p = 0.009), sperm concentration (β −3.5, p = 0.003) and total sperm count (β −3.2, p = 0.007). The injection of hCG lowered sLHCGR in serum and urine of healthy men (p < 0.01). In conclusion, sLHCGR is released into body-fluids and linked with pubertal development and gonadal function. Circulating sLHCGR in anorchid men suggests that sLHCGR in serum may originate from and possibly exert actions in non-gonadal tissues. (ClinicalTrials: NTC01411527, NCT01304927, NCT03418896).

scholarly journals Expression of Stem Cell Markers in the Human Fetal Kidney

PLoS ONE ◽  
2009 ◽  
Vol 4 (8) ◽  
pp. e6709 ◽  
Author(s):  
Sally Metsuyanim ◽  
Orit Harari-Steinberg ◽  
Ella Buzhor ◽  
Dorit Omer ◽  
Naomi Pode-Shakked ◽  
...  
Keyword(s):  
Stem Cell ◽  
Stem Cell Markers ◽  
Fetal Kidney ◽  
Cell Markers ◽  
Human Fetal Kidney

scholarly journals Spatial relationship between expression of cytokeratin-19 and that of connexin-43 in human fetal kidney

2013 ◽  
Vol 46 (1) ◽  
pp. 32 ◽  
Author(s):  
Keisuke Hieda ◽  
Shogo Hayashi ◽  
Ji Hyun Kim ◽  
Gen Murakami ◽  
Baik Hwan Cho ◽  
...  
Keyword(s):  
Connexin 43 ◽  
Spatial Relationship ◽  
Cytokeratin 19 ◽  
Fetal Kidney ◽  
Human Fetal Kidney

Growth and development of the human fetal kidney

1986 ◽  
Vol 53 (2) ◽  
pp. 273-279 ◽  
Author(s):  
G. K. Mehrotra ◽  
G. Datta ◽  
K. L. Mukherjee
Keyword(s):  
Growth And Development ◽  
Fetal Kidney ◽  
Human Fetal Kidney

scholarly journals Expression of the Von Hippel-Lindau Tumor Suppressor Gene, VHL, in Human Fetal Kidney and During Mouse Embryogenesis

1995 ◽  
Vol 1 (4) ◽  
pp. 457-466 ◽  
Author(s):  
Patricia M. Kessler ◽  
Sandip P. Vasavada ◽  
Raymond R. Rackley ◽  
Thomas Stackhouse ◽  
Fuh-Mei Duh ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Tumor Suppressor Gene ◽  
Suppressor Gene ◽  
Fetal Kidney ◽  
Mouse Embryogenesis ◽  
Von Hippel Lindau ◽  
Human Fetal Kidney

scholarly journals Single-cell transcriptomics reveals gene expression dynamics of human fetal kidney development

PLoS Biology ◽  
2019 ◽  
Vol 17 (2) ◽  
pp. e3000152 ◽  
Author(s):  
Mazène Hochane ◽  
Patrick R. van den Berg ◽  
Xueying Fan ◽  
Noémie Bérenger-Currias ◽  
Esmée Adegeest ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Kidney Development ◽  
Fetal Kidney ◽  
Gene Expression Dynamics ◽  
Human Fetal Kidney

scholarly journals Transplantation of Fetal Kidney Tissue Reduces Cerebral Infarction Induced by Middle Cerebral Artery Ligation

1999 ◽  
Vol 19 (12) ◽  
pp. 1329-1335 ◽  
Author(s):  
Yung-Hsiao Chiang ◽  
Shinn-Zong Lin ◽  
Cesario V. Borlongan ◽  
Barry J. Hoffer ◽  
Marisela Morales ◽  
...  
Keyword(s):  
Cerebral Cortex ◽  
Kidney Transplantation ◽  
Middle Cerebral Artery ◽  
Mrna Expression ◽  
Kidney Tissue ◽  
Kidney Cortex ◽  
Fetal Kidney ◽  
Adult Rats ◽  
Adult Kidney ◽  
The Right

The authors, and others, have recently reported that intracerebral administration of glial cell line-derived neurotrophic factor (GDNF) or osteogenic protein-1 protects against ischemia-induced injury in the cerebral cortex of adult rats. Because these trophic factors are highly expressed in the fetal, but not adult, kidney cortex, the possibility that transplantation of fetal kidney tissue could serve as a cellular reservoir for such molecules and protect against ischemic injury in cerebral cortex was examined. Fetal kidneys obtained from rat embryos at gestational day 16, and adult kidney cortex, were dissected and cut into small pieces. Adult male Sprague-Dawley rats were anesthetized with chloral hydrate and placed in a stereotactic apparatus. Kidney tissues were transplanted into three cortical areas adjacent to the right middle cerebral artery (MCA). Thirty minutes after grafting, the right MCA was transiently ligated for 90 minutes. Twenty-four hours after the onset of reperfusion, animals were evaluated behaviorally. It was found that the stroke animals that received adult kidney transplantation developed motor imbalance. However, animals that received fetal kidney grafts showed significant behavioral improvement. Animals were later sacrificed and brains were removed for triphenyltetrazolium chloride staining, Pax-2 immunostaining, and GDNF mRNA expression. It was noted that transplantation of fetal kidney but not adult kidney tissue greatly reduced the volume of infarction in the cerebral cortex. Fetal kidney grafts showed Pax-2 immunoreactivity and GDNF mRNA in the host cerebral cortex. In contrast, GDNF mRNA expression was not found in the adult kidney grafts. Taken together, our data indicate that fetal kidney transplantation reduces ischemia/reperfusion-induced cortical infarction and behavioral deficits in adult rats, and that such tissue grafts could serve as an unique cellular reservoir for trophic factor application to the brain.

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