scholarly journals Analysis of the mutant picornavirus RNA-dependent RNA polymerase lacking catalytic activity (RdRPΔcat)-dependent transcriptome in human THP-1 monocytic leukemia cells

2019 ◽  
Author(s):  
EM Poeschla
Keyword(s):  
Catalytic Activity ◽  
Rna Polymerase ◽  
Leukemia Cells ◽  
Monocytic Leukemia ◽  
Rna Dependent Rna Polymerase

scholarly journals The Pol IV largest subunit CTD quantitatively affects siRNA levels guiding RNA-directed DNA methylation

2019 ◽  
Vol 47 (17) ◽  
pp. 9024-9036 ◽  
Author(s):  
Jered M Wendte ◽  
Jeremy R Haag ◽  
Olga M Pontes ◽  
Jasleen Singh ◽  
Sara Metcalf ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Catalytic Activity ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Cytosine Methylation ◽  
Transcriptional Gene Silencing ◽  
Rna Dependent Rna Polymerase ◽  
Pol Ii ◽  
Pol Iv ◽  
Non Coding Rnas

Abstract In plants, nuclear multisubunit RNA polymerases IV and V are RNA Polymerase II-related enzymes that synthesize non-coding RNAs for RNA-directed DNA methylation (RdDM) and transcriptional gene silencing. Here, we tested the importance of the C-terminal domain (CTD) of Pol IV’s largest subunit given that the Pol II CTD mediates multiple aspects of Pol II transcription. We show that the CTD is dispensable for Pol IV catalytic activity and Pol IV termination-dependent activation of RNA-DEPENDENT RNA POLYMERASE 2, which partners with Pol IV to generate dsRNA precursors of the 24 nt siRNAs that guide RdDM. However, 24 nt siRNA levels decrease ∼80% when the CTD is deleted. RNA-dependent cytosine methylation is also reduced, but only ∼20%, suggesting that siRNA levels typically exceed the levels needed for methylation of most loci. Pol IV-dependent loci affected by loss of the CTD are primarily located in chromosome arms, similar to loci dependent CLSY1/2 or SHH1, which are proteins implicated in Pol IV recruitment. However, deletion of the CTD does not phenocopy clsy or shh1 mutants, consistent with the CTD affecting post-recruitment aspects of Pol IV activity at target loci.

scholarly journals Isolation of an RNA-dependent RNA Polymerase from Friend Murine Leukemia Cells

1970 ◽  
Vol 46 (10) ◽  
pp. 1016-1021 ◽  
Author(s):  
Ichiro HARUNA ◽  
Tsuneya OHNO ◽  
Itaru WATANABE ◽  
Yoji IKAWA
Keyword(s):  
Rna Polymerase ◽  
Leukemia Cells ◽  
Rna Dependent Rna Polymerase ◽  
Murine Leukemia

scholarly journals Oligomeric interaction of hepatitis C virus NS5B is critical for catalytic activity of RNA-dependent RNA polymerase.

2015 ◽  
Vol 290 (36) ◽  
pp. 22310-22310
Author(s):  
Weiping Qin ◽  
Hong Luo ◽  
Takahiro Nomura ◽  
Naoyuki Hayashi ◽  
Tatsuya Yamashita ◽  
...  
Keyword(s):  
Catalytic Activity ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Rna Polymerase ◽  
Rna Dependent Rna Polymerase
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