scholarly journals Analysis of the rSARS-CoV-dependent transcriptome in green monkey kidney epitelial cells

SPP Datasets ◽  
2020 ◽  
Author(s):  
ML DeDiego
Keyword(s):  
Monkey Kidney ◽  
Green Monkey

Surface expression of influenza virus neuraminidase, an amino-terminally anchored viral membrane glycoprotein, in polarized epithelial cells

1985 ◽  
Vol 5 (9) ◽  
pp. 2181-2189
Author(s):  
L V Jones ◽  
R W Compans ◽  
A R Davis ◽  
T J Bos ◽  
D P Nayak
Keyword(s):  
Influenza Virus ◽  
Influenza A ◽  
Surface Expression ◽  
African Green Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
African Green Monkey Kidney ◽  
Amino Terminal ◽  
Green Monkey ◽  
Na Gene

We have investigated the site of surface expression of the neuraminidase (NA) glycoprotein of influenza A virus, which, in contrast to the hemagglutinin, is bound to membranes by hydrophobic residues near the NH2-terminus. Madin-Darby canine kidney or primary African green monkey kidney cells infected with influenza A/WSN/33 virus and subsequently labeled with monoclonal antibody to the NA and then with a colloidal gold- or ferritin-conjugated second antibody exhibited specific labeling of apical surfaces. Using simian virus 40 late expression vectors, we also studied the surface expression of the complete NA gene (SNC) and a truncated NA gene (SN10) in either primary or a polarized continuous line (MA104) of African green monkey kidney cells. The polypeptides encoded by the cloned NA cDNAs were expressed on the surface of both cell types. Analysis of [3H]mannose-labeled polypeptides from recombinant virus-infected MA104 cells showed that the products of cloned NA cDNA comigrated with glycosylated NA from influenza virus-infected cells. Both the complete and the truncated glycoproteins were found to be preferentially expressed on apical plasma membranes, as detected by immunogold labeling. These results indicate that the NA polypeptide contains structural features capable of directing the transport of the protein to apical cell surfaces and the first 10 amino-terminal residues of the NA polypeptide are not involved in this process.

The Effects of Some Metabolic Inhibitors on the Ability of SV 40 Virus in Transformed Cells to be Detected by Cell Fusion

1970 ◽  
Vol 6 (3) ◽  
pp. 721-737
Author(s):  
J. F. WATKINS
Keyword(s):  
Cell Fusion ◽  
Monkey Kidney ◽  
New Method ◽  
Metabolic Inhibitors ◽  
Transformed Cells ◽  
Kidney Cells ◽  
African Green Monkey Kidney ◽  
Green Monkey ◽  
Mouse Cells ◽  
Sv 40 Virus

When mouse fibroblasts transformed by SV 40 virus are fused with African Green Monkey kidney cells about 5% of the heterokaryons produce infective SV 40 virus. If the transformed mouse cells are treated with iododeoxyuridine before fusion, the percentage of heterokaryons producing SV 40 virus is increased to between 50 and 80%; if the transformed cells are treated with 8-azaguanine, the percentage of heterokaryons yielding virus is increased to between 59% and 96%. This shows that whether the SV 40 virus can be detected by cell fusion depends on the state of the transformed cells beforehand. A new method for titrating SV 40 virus is described.

Recombination between irradiated shuttle vector DNA and chromosomal DNA in African green monkey kidney cells

1990 ◽  
Vol 10 (1) ◽  
pp. 37-46
Author(s):  
J S Mudgett ◽  
W D Taylor
Keyword(s):  
Gamma Irradiation ◽  
Uv Light ◽  
Shuttle Vector ◽  
Monkey Kidney ◽  
Host Cells ◽  
Mammalian Host ◽  
Kidney Cells ◽  
African Green Monkey Kidney ◽  
Green Monkey ◽  
Chromosome Recombination

An autonomously replicating shuttle vector was used to investigate enhancement of plasmid-chromosome recombination in mammalian host cells by gamma irradiation and UV light. Sequences homologous to the shuttle vector were stably inserted into the genome of African green monkey kidney cells to act as the target substrate for these recombination events. The shuttle vector molecules were irradiated at various doses before transfection into the mammalian host cells that contained the stable insertions. The homologous transfer of the bacterial ampicillin resistance gene from the inserted sequences to replace a mutant ampicillin sensitivity gene on the shuttle vector was identified by the recovery of ampicillin-resistant plasmids after Hirt extraction and transformation into Escherichia coli host cells. Gamma irradiation increased homologous shuttle vector-chromosome recombination, whereas UV light did not increase the frequency of recombinant plasmids detected. Introducing specific double-strand breaks in the plasmid or prolonging the time of plasmid residence in the mammalian host cells also enhanced plasmid-chromosome recombination. In contrast, plasmid mutagenesis was increased by UV irradiation of the plasmid but did not change with time. The ampicillin-resistant recombinant plasmid molecules analyzed appeared to rise mostly from nonconservative exchanges that involved both homologous and possibly nonhomologous interactions with the host chromosome. The observation that similar recombinant structures were obtained from all the plasmid treatments and host cells used suggests a common mechanism for plasmid-chromosome recombination in these mammalian cells.

Assessment of potential miRNA biomarkers of VERO-cell tumorigenicity in a new line (AGMK1-9T7) of African green monkey kidney cells

Vaccine ◽  
2017 ◽  
Vol 35 (41) ◽  
pp. 5503-5509 ◽  
Author(s):  
Belete Teferedegne ◽  
Daniel M. Rotroff ◽  
Juliete Macauley ◽  
Gideon Foseh ◽  
Gladys Lewis ◽  
...  
Keyword(s):  
Vero Cell ◽  
African Green Monkey ◽  
Monkey Kidney ◽  
Kidney Cells ◽  
African Green Monkey Kidney ◽  
Green Monkey
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