scholarly journals General Characteristics of the Cells Transformed in vitro by Human Adenovirus Type 12

1967 ◽  
Vol 92 (2) ◽  
pp. 151-160 ◽  
Author(s):  
Toshihisa Kusano ◽  
Isao Yamane
Keyword(s):  
Human Adenovirus ◽  
Adenovirus Type ◽  
Human Adenovirus Type 12

scholarly journals In Vitro and In Vivo Evaluation of Human Adenovirus Type 49 as a Vector for Therapeutic Applications

Viruses ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 1483
Author(s):  
Emily A. Bates ◽  
John R. Counsell ◽  
Sophie Alizert ◽  
Alexander T. Baker ◽  
Natalie Suff ◽  
...  
Keyword(s):  
Viral Vector ◽  
Ex Vivo ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Cell Types ◽  
In Vivo Evaluation ◽  
In Vivo Biodistribution ◽  
Adenovirus Type 5

The human adenovirus phylogenetic tree is split across seven species (A–G). Species D adenoviruses offer potential advantages for gene therapy applications, with low rates of pre-existing immunity detected across screened populations. However, many aspects of the basic virology of species D—such as their cellular tropism, receptor usage, and in vivo biodistribution profile—remain unknown. Here, we have characterized human adenovirus type 49 (HAdV-D49)—a relatively understudied species D member. We report that HAdV-D49 does not appear to use a single pathway to gain cell entry, but appears able to interact with various surface molecules for entry. As such, HAdV-D49 can transduce a broad range of cell types in vitro, with variable engagement of blood coagulation FX. Interestingly, when comparing in vivo biodistribution to adenovirus type 5, HAdV-D49 vectors show reduced liver targeting, whilst maintaining transduction of lung and spleen. Overall, this presents HAdV-D49 as a robust viral vector platform for ex vivo manipulation of human cells, and for in vivo applications where the therapeutic goal is to target the lung or gain access to immune cells in the spleen, whilst avoiding liver interactions, such as intravascular vaccine applications.

Immunofluorescence of Human Adenovirus Type 12 in Various Cell Types.

1966 ◽  
Vol 121 (2) ◽  
pp. 405-414 ◽  
Author(s):  
J. D. Levinthal ◽  
C. Ahmad-Zadeh ◽  
G. Van Hoosier ◽  
J. J. Trentin
Keyword(s):  
Human Adenovirus ◽  
Adenovirus Type ◽  
Cell Types ◽  
Human Adenovirus Type 12
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