Growth Comparison Between Lymphocyte BW5147 T-cells and EL4 T-cells Using Atmospheric Oxygen Plasma Irradiation

2021 ◽  
Author(s):  
Sitti Subaedah ◽  
Haruka Uematsu ◽  
Nobuya Hayashi
2020 ◽  
Vol 59 (SJ) ◽  
pp. SJJF03
Author(s):  
Sitti Subaedah ◽  
Haruka Uematsu ◽  
Nobuya Hayashi

2015 ◽  
Vol 26 (29) ◽  
pp. 295204 ◽  
Author(s):  
Yingcui Fang ◽  
Bing Zhang ◽  
Liu Hong ◽  
Damao Yao ◽  
Zhiqiang Xie ◽  
...  

2012 ◽  
Vol 629 ◽  
pp. 19-24 ◽  
Author(s):  
Cheng Yu Wang ◽  
Fei Shi ◽  
Shi Hong Pang ◽  
Chun Sheng Ren ◽  
Ying Tao ◽  
...  

Coatings such as Cr, Ni, Ti, and SiO2/SnO2 on solar-control and low-emissivity (low-E) glasses are commonly used in the energy efficient glass windows. However, coloring in the re-manufactured glass panels using recycled window glasses resulting from the coatings reduces the glass transparency significantly. Traditional ways to remove coatings by manual wheels and pneumatic removal machines are labor intense and slow processes. In this study a new way to remove the coatings on recycled coated glasses was investigated. The Ultra Violet/Visible/Infrared (UV/VIS/IR) spectra and Secondary Electron Microscope (SEM) pictures were taken before and after 30s. atmospheric pressure oxygen plasma treatments. It was confirmed that the atmospheric oxygen plasma treatment is a fast, efficient, and low pollution way to remove the coatings before the remanufacture of recycled glasses. Other than the physical sputtering off the coatings from the plasma, there are many excited oxygen species in the plasma which effectively react to the coating and the products are then removed.


2020 ◽  
Author(s):  
Amijai Saragovi ◽  
Ifat Abramovitch ◽  
Ibrahim Omar ◽  
Eliran Arbib ◽  
Ori Toker ◽  
...  

SUMMARYSystemic oxygen restriction (SOR) is prevalent in numerous clinical conditions including chronic obstructive pulmonary disease (COPD). However, the influence of SOR on T cell protective immunity remains uncharacterized. Here we show the detrimental effect of hypoxia on mitochondrial biogenesis in activated CD8+ T cells. We find that low oxygen diminishes CD8+ T cell viral response in vivo. Using genetic and pharmacological models, we demonstrate that respiratory restriction inhibits ATP dependent matrix processes, all critical for mitochondrial biogenesis. The effect mediated by respiratory restriction could be rescued by TCA cycle re-stimulation, which led to increased mitochondrial matrix localized ATP via substrate-level phosphorylation. Finally, we demonstrate that short exposure to atmospheric oxygen pressure rescues the CD8+ viral response under systemic oxygen restriction in vivo. Our findings reveal the detrimental effect of hypoxia on mitochondrial biogenesis in activated CD8+ T cells and provide a new approach for reducing viral infections in COPD.HighlightsSystemic chronic hypoxia compromises CD8+ T cell activationShortly upon activation, T cells’ cytoplasmic activity becomes independent of mitochondrial ATP outfluxRespiratory-blockade arrests mitochondrial remodeling due to energy depletionUncoupler-based TCA stimulation rescues respiratory-restricted activated CD8+ T cells by stimulating matrix localized substrate-level phosphorylationCD8+ T cell arrest due to hypoxia in vivo can be rescued by short exposure to atmospheric oxygen pressure.


Author(s):  
Nobuya Hayashi ◽  
Kyotaro Yamamoto

Gene expression variations of plant leaf are investigated by irradiating seed and leaf with oxygen or air plasmas. Enhancement of leaf growth is induced by oxygen plasma irradiation on seeds, which is supported by increased gene expression for protein synthesis, oxidative-reduction reactions and decreased gene expression concerning DNA methylation and histone modification. Suppression of leaf growth is observed by the oxygen plasma, which would be owing to increased gene expression concerning heat shock protein and redox reaction, and decreased expression of photosynthesis and glycoprotein. Also, gene expression variation due to air plasma irradiation is almost same as that of oxygen plasma. Active oxygen species are major factors in both oxygen and air plasmas for the variation of gene expressions in plant.


2000 ◽  
Vol 657 ◽  
Author(s):  
Yonah Cho ◽  
Nathan W. Cheung

ABSTRACTChemical and physical effects of plasma exposed Si wafer pairs were investigated on Si wafer bonding. Oxygen plasma treated Si wafer pairs bonded more strongly at room temperature compared to chemically cleaned, hydrophilic and hydrophobic Si. After 50 hours of annealing at 105°C, the surface energy of the bonded Si pair reached the surface energy of bulk Si (100). X- ray photoemission spectroscopy (XPS) measurements indicated that the exposure of both hydrophilic and hydrophobic Si to oxygen plasma increased a SiO2 like state in the surface layer to a depth of 1.5 nm. Atomic force microscopy (AFM) study showed that plasma irradiation at 300 watts up to 30 seconds did not change surface roughness below 0.5 nm. Exposure to He plasma or N2 did result in enhanced bonding after annealing at 165°C, however, over smaller areas.


2011 ◽  
Vol 54 (5) ◽  
pp. 317-321 ◽  
Author(s):  
Daisuke NOGUCHI ◽  
Shoji FUKUDOME ◽  
Megumi SHIMONIIHARA ◽  
Yoshihiko KAWANO ◽  
Fumihiro SEI

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