scholarly journals Direct Current Helium Plasma for In vivo Delivery of Plasmid DNA Encoding Erythropoietin to Murine Skin

2017 ◽  
Vol 7 (3) ◽  
pp. 261-271 ◽  
Author(s):  
Mark J. Jaroszeski ◽  
Taryn Harvey-Chapman ◽  
Andrew Hoff ◽  
Reginald Atkins ◽  
Richard J. Connolly
Keyword(s):  
Direct Current ◽  
Plasmid Dna ◽  
Helium Plasma ◽  
Dna Encoding ◽  
In Vivo Delivery

scholarly journals Focused in vivo Delivery of Plasmid DNA to the Porcine Vascular Wall via Intravascular Ultrasound Destruction of Microbubbles

2010 ◽  
Vol 47 (3) ◽  
pp. 270-274 ◽  
Author(s):  
Linsey C. Phillips ◽  
Alexander L. Klibanov ◽  
Douglas K. Bowles ◽  
Michael Ragosta ◽  
John A. Hossack ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Plasmid Dna ◽  
Vascular Wall ◽  
In Vivo Delivery

scholarly journals Magnetic field contributes to the cellular uptake for effective therapy with magnetofection using plasmid DNA encoding against Mcam in B16F10 melanoma in vivo

Nanomedicine ◽  
2016 ◽  
Vol 11 (6) ◽  
pp. 627-641 ◽  
Author(s):  
Lara Prosen ◽  
Samo Hudoklin ◽  
Maja Cemazar ◽  
Monika Stimac ◽  
Ursa Lampreht Tratar ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Cellular Uptake ◽  
Plasmid Dna ◽  
Effective Therapy ◽  
Dna Encoding ◽  
B16f10 Melanoma

scholarly journals 3D Printed Gene-activated Octacalcium Phosphate Implants for Large Bone Defects Engineering

2020 ◽  
Vol 6 (3) ◽  
Author(s):  
Ilya I Bozo ◽  
Roman V. Deev ◽  
Igor V. Smirnov ◽  
Alexander Yu Fedotov ◽  
Vladimir K. Popov ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Three Dimensional ◽  
Bone Substitutes ◽  
Octacalcium Phosphate ◽  
Dna Encoding ◽  
X Ray Diffraction ◽  
3D Printed ◽  
Clinical Potential ◽  
Large Bone

The aim of the study was the development of three-dimensional (3D) printed gene-activated implants based on octacalcium phosphate (OCP) and plasmid DNA encoding VEGFA. The first objective of the present work involved design and fabrication of gene-activated bone substitutes based on the OCP and plasmid DNA with VEGFА gene using 3D printing approach of ceramic constructs, providing the control of its architectonics compliance to the initial digital models. X-ray diffraction, scanning electron microscopy (SEM), Fourier transform infrared spectroscopy, and compressive strength analyses were applied to investigate the chemical composition, microstructure, and mechanical properties of the experimental samples. The biodegradation rate and the efficacy of plasmid DNA delivery in vivo were assessed during standard tests with subcutaneous implantation to rodents in the next stage. The final part of the study involved substitution of segmental tibia and mandibular defects in adult pigs with 3D printed gene-activated implants. Biodegradation, osteointegration, and effectiveness of a reparative osteogenesis were evaluated with computerized tomography, SEM, and a histological examination. The combination of gene therapy and 3D printed implants manifested the significant clinical potential for effective bone regeneration in large/critical size defect cases.

Design of lipid nanoparticles for in vitro and in vivo delivery of plasmid DNA

2017 ◽  
Vol 13 (4) ◽  
pp. 1377-1387 ◽  
Author(s):  
Jayesh A. Kulkarni ◽  
Johnathan Layne Myhre ◽  
Sam Chen ◽  
Yuen Yi C. Tam ◽  
Adrian Danescu ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Lipid Nanoparticles ◽  
In Vivo Delivery

scholarly journals Nuclear-Targeting Delivery of CRISPRa System for Upregulation of β-Defensin against Virus Infection by Dexamethasone and Phenylalanine Dual-Modified Dendrimer

2020 ◽  
Vol 2020 ◽  
pp. 1-13
Author(s):  
Mingxiang Zuo ◽  
Xiaoxia Li ◽  
Shuang Liu ◽  
Bin Chen ◽  
Du Cheng
Keyword(s):  
Virus Infection ◽  
Plasmid Dna ◽  
Transfection Efficiency ◽  
Viral Disease ◽  
Dna Encoding ◽  
Nuclear Targeting ◽  
Guide Rna ◽  
Targeting Delivery ◽  
Vesicular Stomatitis

The dual-modified dendrimer containing dexamethasone (DET) and phenylalanine (Phe) was prepared to deliver plasmid DNA encoding dCas9 and single-guide RNA (sgRNA) for specific upregulation of β-defensin. DET and Phe moieties synergistically enhanced the transfection efficiency and reduced cytotoxicity of dendrimers. Combination of three sgRNAs targeting β-defensin gene demonstrated higher activation efficacy of β-defensin than any single sgRNA and combinations of any two sgRNAs, showing an efficient inhibition of virus infection and replication. The titer of vesicular stomatitis virus (VSV) in the cells treated with dCas9-sgRNA targeting β-defensin was reduced by about 100-fold compared to that of cells treated with dCas9-scramble sgRNA (dCas9-scr sgRNA). In vivo experiments demonstrated that the DET- and Phe-modified dendrimer effectively delivered plasmid DNA encoding dCas9 protein into the airway epithelium, inducing β-defensin expression. Delivery of the CRISPR activation system by a dendrimer modified with DET and Phe was a promising approach against viral disease.

scholarly journals Gemini Cationic Lipid-Type Nanovectors Suitable for the Transfection of Therapeutic Plasmid DNA Encoding for Pro-Inflammatory Cytokine Interleukin-12

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 729
Author(s):  
Natalia Sánchez-Arribas ◽  
María Martínez-Negro ◽  
Clara Aicart-Ramos ◽  
Conchita Tros de Ilarduya ◽  
Emilio Aicart ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Physicochemical Characterization ◽  
Cationic Lipid ◽  
Cationic Lipids ◽  
Interleukin 12 ◽  
Dna Encoding ◽  
Lipid Mixture ◽  
Ample Evidence

Ample evidence exists on the role of interleukin-12 (IL-12) in the response against many pathogens, as well as on its remarkable antitumor properties. However, the unexpected toxicity and disappointing results in some clinical trials are prompting the design of new strategies and/or vectors for IL-12 delivery. This study was conceived to further endorse the use of gemini cationic lipids (GCLs) in combination with zwitterionic helper lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphatidyl ethanol amine) as nanovectors for the insertion of plasmid DNA encoding for IL-12 (pCMV-IL12) into cells. Optimal GCL formulations previously reported by us were selected for IL-12-based biophysical experiments. In vitro studies demonstrated efficient pCMV-IL12 transfection by GCLs with comparable or superior cytokine levels than those obtained with commercial control Lipofectamine2000*. Furthermore, the nanovectors did not present significant toxicity, showing high cell viability values. The proteins adsorbed on the nanovector surface were found to be mostly lipoproteins and serum albumin, which are both beneficial to increase the blood circulation time. These outstanding results are accompanied by an initial physicochemical characterization to confirm DNA compaction and protection by the lipid mixture. Although further studies would be necessary, the present GCLs exhibit promising characteristics as candidates for pCMV-IL12 transfection in future in vivo applications.

scholarly journals CpG-Modified Plasmid DNA Encoding Flagellin Improves Immunogenicity and Provides Protection against Burkholderia pseudomallei Infection in BALB/c Mice

2006 ◽  
Vol 74 (3) ◽  
pp. 1699-1705 ◽  
Author(s):  
Yao-Shen Chen ◽  
Yu-Shan Hsiao ◽  
Hsi-Hsun Lin ◽  
Yin Liu ◽  
Ya-Lei Chen
Keyword(s):  
Plasmid Dna ◽  
Burkholderia Pseudomallei ◽  
Time Course ◽  
Functional Expression ◽  
Dna Encoding ◽  
Phagocytic Cells ◽  
Reverse Transcription Pcr ◽  
Cpg Oligodeoxynucleotide ◽  
Th 1

ABSTRACT The plasmid DNA encoding the fliC gene of Burkholderia pseudomallei combined with CpG oligodeoxynucleotide (ODN) was injected intramuscularly into BALB/c mice, resulting in the increased production of certain humoral antibodies and flagellin-specific spleen cell clonal expansion. CpG ODN, as an immunoadjuvant, was added to the plasmid containing the fliC gene in order to obtain ongoing expression in muscle for a long period. Functional expression of flagellin from the constructed CpG-modified plasmid in transfected peritoneal exudate cells of BALB/c mice was shown by reverse transcription-PCR and Western blotting. Furthermore, BALB/c mice immunized with the modified plasmid had relatively higher resistance to B. pseudomallei infection in vivo than did mice immunized with unmodified plasmid DNA. The time course of restricted bacterial growth in spleen and liver and changes in the cytokine profiles of immunized mice suggested that the stimulated phagocytic cells would be able to kill the bacteria eventually, possibly as a consequence of the induction of Th-1-type immune polarization in vivo. Th-1-type immune polarization was detected in response to flagellin induction in mice immunized with CpG-modified plasmid DNA by the appearance of increased levels of immunoglobulin G2a antibodies and gamma interferon-secreting cells specific to flagellin. The exogenous CpG motifs added to the fliC gene would contribute to an adjuvant-like response that enhances the flagellin-specific immunogenicity and provides protection against B. pseudomallei infection. This CpG-modified plasmid DNA vaccination is an important potential strategy that should be developed to protect against melioidosis.

In Vivo Evaluation of Plasmid DNA Encoding OP-1 Protein for Spine Fusion

Spine ◽  
2006 ◽  
Vol 31 (19) ◽  
pp. 2163-2172 ◽  
Author(s):  
Corinne Bright ◽  
Ye-Soo Park ◽  
Ann N. Sieber ◽  
John P. Kostuik ◽  
Kam W. Leong
Keyword(s):  
Plasmid Dna ◽  
Spine Fusion ◽  
Dna Encoding ◽  
In Vivo Evaluation
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