scholarly journals DNA Microarray Analysis of Plant Seeds Irradiated by Active Oxygen Species in Oxygen Plasma

2016 ◽  
Vol 6 (3-4) ◽  
pp. 459-471 ◽  
Author(s):  
Nobuya Hayashi ◽  
Reoto Ono ◽  
Riku Nakano ◽  
Masaharu Shiratani ◽  
Kosuke Tashiro ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Oxygen Plasma ◽  
Active Oxygen Species ◽  
Active Oxygen ◽  
Oxygen Species ◽  
Dna Microarray Analysis ◽  
Plant Seeds

Characterization and functional properties of new everbearing strawberry (Fragaria x ananasa Duch.) cultivar, ‘Summertiara’ berries

2014 ◽  
Vol 4 (1) ◽  
pp. 1 ◽  
Author(s):  
Takeshi Nagai ◽  
Misuzu Tamai ◽  
Masato Sato ◽  
Yasuhiro Tanoue ◽  
Norihisa Kai ◽  
...  
Keyword(s):  
Vitamin C ◽  
Sensory Evaluation ◽  
Functional Properties ◽  
Active Oxygen Species ◽  
Active Oxygen ◽  
Antihypertensive Activity ◽  
Oxygen Species ◽  
Total Anthocyanins ◽  
Strawberry Cultivar ◽  
The Stability

Background: In recent years, a new everbearing strawberry cultivar, ‘Summertiara’ was cultivated to supply the strawberries in pre-harvest season from July to October in Japan. For highly research and development of processing of this cultivar, ‘Summertiara’ berries, the objective of this study was to characterize these berries, with relation to chemical parameters, total phenols, total flavonoids, total vitamin C, and total anthocyanins, and was to investigate the solubility and the stability of anthocyanins from the berries. Moreover, the functional properties such as antioxidative activity, active oxygen species scavenging activity, and antihypertensive activity were also evaluated.Methods: Chemical analysis, colour measurement, and sensory evaluation of new everbearing strawberry cultivar, ‘Summertiara’ berries were performed. Next, the solubility of anthocyanins from the berries and stability of these against pH, temperature, and an incandescent lighting were investigated. Moreover, functional properties of the extracts prepared from berries were elucidated using 5 different methods.Results: The contents of water, proteins, lipids, carbohydrates, and ash were the same as those of other cultivar berries. The sugar-acid ratio in the berries was low; these were acidulous. By sensory evaluation, the main factors were vivid red colour, aroma, and acidity. The berries were rich in phenols, flavonoids, vitamin C, and anthocyanins. The anthocyanins of the berries became unstable by heat treatment and light exposures such as visible rays. On the other hand, the extracts prepared from the berries showed the functionalities such as antioxidant activity, active oxygen species scavenging activities, and antihypertensive activity.  Conclusions: The strawberry cultivar, ‘Summertiara’ berries were the most suitable for processing ingredient of strawberry-derived products with superior health promoting functionalities.Keywords: Summertiara, everbearing strawberry cultivar, characterization, sensory evaluation, color and storage, functional property

scholarly journals The Potential of 2-aza-8-Oxohypoxanthine as a Cosmetic Ingredient

Cosmetics ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 60
Author(s):  
Hisae Aoshima ◽  
Masayuki Ito ◽  
Rinta Ibuki ◽  
Hirokazu Kawagishi
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Cell Activation ◽  
Skin Barrier ◽  
Efficacy Evaluation ◽  
Activation Effect ◽  
Expression Levels ◽  
Dna Microarray Analysis

In this study, we verified the effects of 2-aza-8-oxohypoxanthine (AOH) on human epidermal cell proliferation by performing DNA microarray analysis. Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which measures mitochondrial respiration in normal human epidermal keratinocyte (NHEK) cells. Gene expression levels were determined by DNA microarray analysis of 177 genes involved in skin aging and disease. AOH showed a significant increase in cell viability at concentrations between 7.8 and 31.3 μg/mL and a significant decrease at concentrations above 250 μg/mL. DNA microarray analysis showed that AOH significantly increased the gene expression of CLDN1, DSC1, DSG1, and CDH1 (E-cadherin), which are involved in intercellular adhesion and skin barrier functioning. AOH also up-regulated the expression of KLK5, KLK7, and SPIMK5, which are proteases involved in stratum corneum detachment. Furthermore, AOH significantly stimulated the expression of KRT1, KRT10, TGM1, and IVL, which are considered general differentiation indicators, and that of SPRR1B, a cornified envelope component protein. AOH exerted a cell activation effect on human epidermal cells. Since AOH did not cause cytotoxicity, it was considered that the compound had no adverse effects on the skin. In addition, it was found that AOH stimulated the expression levels of genes involved in skin barrier functioning by DNA microarray analysis. Therefore, AOH has the potential for practical use as a cosmetic ingredient. This is the first report of efficacy evaluation tests performed for AOH.

Identifying the Active Oxygen Species in SnO2 Based Gas Sensing Materials: An Operando IR Spectrsocopy Study

2015 ◽  
Vol 119 (21) ◽  
pp. 11792-11799 ◽  
Author(s):  
David Degler ◽  
Susanne Wicker ◽  
Udo Weimar ◽  
Nicolae Barsan
Keyword(s):  
Gas Sensing ◽  
Active Oxygen Species ◽  
Active Oxygen ◽  
Oxygen Species ◽  
Ir Spectrsocopy

Chromosome damage caused by enzymically generated active oxygen species

1983 ◽  
Vol 18 ◽  
pp. 47
Author(s):  
B.J. Phillips ◽  
T.E.B. James ◽  
D. Anderson
Keyword(s):  
Active Oxygen Species ◽  
Active Oxygen ◽  
Chromosome Damage ◽  
Oxygen Species

scholarly journals DNA Microarray Analysis Identifies CKS2 and LEPR as Potential Markers of Meningioma Recurrence

2011 ◽  
Vol 16 (10) ◽  
pp. 1440-1450 ◽  
Author(s):  
Francesca Menghi ◽  
Francesca N. Orzan ◽  
Marica Eoli ◽  
Mariangela Farinotti ◽  
Emanuela Maderna ◽  
...  
Keyword(s):  
Microarray Analysis ◽  
Dna Microarray ◽  
Dna Microarray Analysis ◽  
Meningioma Recurrence
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