Genetic Predisposition to Essential Hypertension in Children: Analysis of 17 Single Nucleotide Polymorphisms

2014 ◽  
Vol 5 (4) ◽  
pp. 307-322
Author(s):  
Sergii V. Goncharov ◽  
Veronika L. Gurianova ◽  
Dmytro O. Stro ◽  
Tatyana I. Drevytska ◽  
Sergii P. Kaplinskii ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Single Nucleotide Polymorphisms ◽  
Genetic Predisposition ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Hypertension In Children

scholarly journals Genetic predisposition to essential hypertension in children: analysis of 17 single nucleotide polymorphisms

2014 ◽  
Vol 59 (6) ◽  
pp. 12-24 ◽  
Author(s):  
Goncharov SV Goncharov SV ◽  
◽  
Gurianova VL Gurianova VL ◽  
Stroy DO Stroy DO ◽  
Drevytska TI Drevytska TI ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Single Nucleotide Polymorphisms ◽  
Genetic Predisposition ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Hypertension In Children

scholarly journals Genotyping of Essential Hypertension Single-Nucleotide Polymorphisms by a Homogeneous PCR Method with Universal Energy Transfer Primers

2002 ◽  
Vol 48 (12) ◽  
pp. 2131-2140 ◽  
Author(s):  
Chikh Bengra ◽  
Theodore E Mifflin ◽  
Yuri Khripin ◽  
Paolo Manunta ◽  
Scott M Williams ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Single Nucleotide Polymorphisms ◽  
Restriction Endonucleases ◽  
Control Group ◽  
Italian Population ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Hypertensive Patients ◽  
Unique Restriction ◽  
Set Up

Abstract Background: Human hypertension is a complex, multifactorial disease with a heritability of more than 30–50%. A genetic screening test based on analysis of multiple single-nucleotide polymorphisms (SNPs) to assess the likelihood of developing hypertension would be helpful for disease management. Methods: Tailed allele-specific primers were designed to amplify by PCR six biallelic SNP loci [three in G protein-coupled receptor kinase type 4 (GRK4): R65L, A142V, and A486V; two in angiotensinogen: −6G→A and M235T; and one in aldosterone synthase: −344C→T] associated with essential hypertension. PCRs of SNP loci were coupled (via a common sequence of 21 nucleotide tails) to incorporate Universal Amplifluor™ primers labeled with fluorescein or sulforhodamine in a homogeneous format. Use of Amplifluors in SNP PCRs produced labeled amplicons, the fluorescence of which was quantified by a microplate reader and then analyzed via an Excel macro to provide genotypes for all six SNP loci. Unique restriction endonucleases were identified for five SNP loci that could independently confirm homogeneous PCR results when needed. Results: We developed six homogeneous PCR assays that were set up, performed, and fluorometrically analyzed in 96-well microplates. Allele frequencies were determined for six SNPs in 60 Italian hypertensive patients and a control group of 60 normotensive persons. A significant correlation (P = 0.034) between one SNP [GRK4 (A486V)] and the hypertensive patients was observed. Genotyping results for five of six SNPs were confirmed by digesting corresponding amplicons with locus-specific restriction endonucleases. Conclusions: We developed a simple and homogeneous fluorescent protocol that has been used to determine the SNP genotype for six loci in a population of hypertensive and normotensive persons. We also observed a significant association (P = 0.034) between one SNP (A486V) and an Italian population of mildly hypertensive patients.

SUSCEPTIBLE SINGLE NUCLEOTIDE POLYMORPHISMS TO RENAL DAMAGE IN PATIENTS WITH ESSENTIAL HYPERTENSION

2004 ◽  
Vol 22 (Suppl. 1) ◽  
pp. S182
Author(s):  
Y Kawano ◽  
K Karnide ◽  
S Takiuchi ◽  
M Yoshii ◽  
Y Miwa ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Single Nucleotide Polymorphisms ◽  
Renal Damage ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

The Genetic Predisposition Score of Seven Obesity-Related Single Nucleotide Polymorphisms Is Associated with Better Metabolic Outcomes after Roux-en-Y Gastric Bypass

2016 ◽  
Vol 9 (5-6) ◽  
pp. 222-230 ◽  
Author(s):  
Carolina Ferreira Nicoletti ◽  
Marcela A. Souza Pinhel ◽  
Bruno Affonso Parenti de Oliveira ◽  
Julio Sergio Marchini ◽  
Wilson Salgado Junior ◽  
...  
Keyword(s):  
Gastric Bypass ◽  
Single Nucleotide Polymorphisms ◽  
Genetic Predisposition ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Metabolic Outcomes ◽  
Genetic Predisposition Score
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