Prophylactic Effect of Probiotic Strains Bifidobacterium Animalis VKL and VKB on Stress-Induced Lesions in the Gastric Mucosa of Rats

2014 ◽  
Vol 5 (1) ◽  
pp. 73-82
Author(s):  
Mykola Ya. Spivak ◽  
L. M. Lazarenko ◽  
Tetyana M. Falalyeyeva ◽  
O. V. Virchenko ◽  
K. S. Neporada
Keyword(s):  
Gastric Mucosa ◽  
Prophylactic Effect ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains

scholarly journals Prophylactic effect of probiotic strains Bifidobacterium animalis VKL and VKB on stress-induced lesions in the gastric mucosa of rats

2013 ◽  
Vol 59 (2) ◽  
pp. 23-30 ◽  
Author(s):  
MIa Spivak ◽  
◽  
LM Lazarenko ◽  
TM Falalieieva ◽  
OV Virchenko ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Prophylactic Effect ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains

scholarly journals Prophylactic effect of lactobacilli and bifidobacteria probiotic strains on experimental bacterial vaginitis

2019 ◽  
Vol 27 (2) ◽  
pp. 170-176
Author(s):  
L. P. Babenko ◽  
L. M. Lazarenko ◽  
R. V. Bubnov ◽  
M. J. Spivak
Keyword(s):  
Lactobacillus Casei ◽  
Inflammatory Diseases ◽  
Coliform Bacteria ◽  
Prophylactic Effect ◽  
Microscopic Fungi ◽  
Preventive Effect ◽  
Genitourinary System ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains ◽  
Spread Of Infection

The objective of the study was determining the prophylactic effect of Lactobacillus casei IMV B-7280, L. acidophilus IMV B-7279, L. delbrueckii subsp. bulgaricus IMV B-7281, Bifidobacterium animalis VKL and B. animalis VKB probiotic strains on experimental vaginitis in BALB/c mice induced by Staphylococcus aureus 8325-4. The infection with S. aureus 8325-4 caused an imbalance of microbiota in the vagina and intestine, as evidenced by an increase in the number of opportunistic microorganisms and a decrease in the amount of lactobacilli and bifidobacteria. L. casei IMV B-7280, B. animalis VKL and B. animalis VKB probiotic strains altered the microbiota spectrum of the vagina and intestine of Staphylococcus-infected mice: the amount of Lactobacillus and bifidobacteria increased with the reduction of the number of opportunistic microorganisms. Also under the influence of these strains, the normalization of the microbiota spectrum typical for vagina and intestine was observed in different periods of observation – in the intestines of mice the number of coliform bacteria increased, the number of microscopic fungi, streptococci and staphylococci decreased; in the vagina, the number of coliform bacteria and microscopic fungi decreased, the number of streptococci normalized. Rapid elimination of S. aureus 8325-4 from the vagina and prevention of the spread of infection to the intestine were observed after use of probiotics. Preventive effect of L. acidophilus IMV B-7279 and L. delbrueckii subsp. bulgaricus IMV B-7281 for bacterial vaginitis in mice was less effective. So, the target probiotic strains L. casei IMV B-7280, B. animalis VKL and B. animalis VKB are promising for the creation of highly effective novel probiotic drugs that can be used for directed prevention of infectious and inflammatory diseases of the genitourinary system caused by pathogenic and opportunistic microorganisms.

scholarly journals Viable and Heat-Killed Probiotic Strains Improve Oral Immunity by Elevating the IgA Concentration in the Oral Mucosa

2021 ◽  
Author(s):  
Wen-Yang Lin ◽  
Yi-Wei Kuo ◽  
Ching-Wei Chen ◽  
Yu-Fen Huang ◽  
Chen-Hung Hsu ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
The Body ◽  
Effective Dosage ◽  
Tgf Beta ◽  
Oral Tablet ◽  
Beneficial Effects ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains ◽  
Oral Pathogens

AbstractOral-nasal mucosal immunity plays a crucial role in protecting the body against bacterial and viral invasion. Safe probiotic products have been used to enhance human immunity and oral health. In this study, we verified the beneficial effects of mixed viable probiotic tablets, consisting of Lactobacillus salivarius subsp. salicinius AP-32, Bifidobacterium animalis subsp. lactis CP-9, and Lactobacillus paracasei ET-66, and heat-killed probiotic tablets, consisting of L. salivarius subsp. salicinius AP-32 and L. paracasei ET-66, on oral immunity among 45 healthy participants. Participants were randomly divided into viable probiotic, heat-killed probiotic, and placebo groups. The administration of treatment lasted for 4 weeks. Saliva samples were collected at Weeks 0, 2, 4, and 6, and Lactobacillus, Bifidobacterium and Streptococcus mutans populations and IgA concentration were measured. IgA concentrations, levels of TGF-beta and IL-10 in PBMCs cells were quantified by ELISA method. Results showed that salivary IgA levels were significantly increased on administration of both the viable (119.30 ± 12.63%, ***P < 0.001) and heat-killed (116.78 ± 12.28%, ***P < 0.001) probiotics for 4 weeks. Among three probiotic strains, AP-32 would effectively increase the levels of TGF-beta and IL-10 in PBMCs. The oral pathogen Streptococcus mutans was significantly reduced on viable probiotic tablet administration (49.60 ± 31.01%, ***P < 0.001). The in vitro antibacterial test confirmed that viable probiotics effectively limited the survival rate of oral pathogens. Thus, this clinical pilot study demonstrated that oral probiotic tablets both in viable form or heat-killed form could exert beneficial effects on oral immunity via IL-10, TGB-beta mediated IgA secretion. The effective dosage of viable probiotic content in the oral tablet was 109 CFUs/g and the heat-killed oral tablet was 1 × 1010 cells/g.

scholarly journals Exopolysaccharides Produced by Probiotic Strains Modify the Adhesion of Probiotics and Enteropathogens to Human Intestinal Mucus

2006 ◽  
Vol 69 (8) ◽  
pp. 2011-2015 ◽  
Author(s):  
PATRICIA RUAS-MADIEDO ◽  
MIGUEL GUEIMONDE ◽  
ABELARDO MARGOLLES ◽  
CLARA G. de los REYES-GAVILÁN ◽  
SEPPO SALMINEN
Keyword(s):  
Salmonella Enterica Serovar Typhimurium ◽  
Bifidobacterium Longum ◽  
Lactobacillus Rhamnosus ◽  
Physiological Role ◽  
Adhesion Properties ◽  
Bifidobacterium Animalis ◽  
Intestinal Mucus ◽  
Serovar Typhimurium ◽  
Probiotic Strains ◽  
Encoding Genes

Exopolysaccharides (EPSs) are exocellular polymers present in the surface of many bacteria, including Lactobacillus and Bifidobacterium. The genome sequence of several strains revealed the presence of EPS-encoding genes. However, the physiological role that EPSs play in the bacterial ecology still remains uncertain. In this study, we have assessed the effect of EPSs produced by Lactobacillus rhamnosus GG, Bifidobacterium longum NB667, and Bifidobacterium animalis IPLA-R1 on the adhesion of probiotic and enteropathogen strains to human intestinal mucus. The EPS fraction GG had no significant effect on the adhesion of L. rhamnosus GG and B. animalis IPLA-R1. However, the EPS fractions NB667 and IPLA-R1 significantly reduced the adherence of both probiotic strains. In contrast, the three EPS fractions increased the adhesion of Enterobacter sakazakii ATCC 29544 and Escherichia coli NCTC 8603. Higher adherence of Salmonella enterica serovar Typhimurium ATCC 29631 and Clostridium difficile ATCC 9689 was detected in the presence of the EPS fractions GG and NB667. In general, these effects were obtained at EPS concentrations of up to 5 mg/ml, and they were EPS dose dependent. The competitive exclusion of probiotics in the presence of EPS could suggest the involvement of these biopolymers in the adhesion to mucus. The increase in the adherence of enteropathogens could be explained if components of the pathogen surface are able to bind to specific EPSs and the bound EPSs are able to adhere to mucus. To the best of our knowledge, this is the first work reporting the effect of EPSs from probiotics on bacterial adhesion properties.

scholarly journals ANTIHERPETIC EFFICACY OF LACTOBACILLI AND BIFIDOBACTERIA PROBIOTIC STRAINS IN EXPERIMENTAL GENITAL HERPES IN GUINEA PIGS

2019 ◽  
pp. 34-44
Author(s):  
D. B. Starosyla ◽  
L. M. Lazarenko ◽  
S.M. Hryhorieva ◽  
L. P. Babenko ◽  
S. L. Rybalko ◽  
...  
Keyword(s):  
Genital Herpes ◽  
Guinea Pigs ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains

Метою було визначення антигерпетичної ефективності пробіотичного штаму Lacto­bacillus casei ІМВ В-7280 та композиції L. caseі ІМВ В-7280 – Bifidobacterium animalis VKL – B. animalis VKВ (В-7280 – VKL – VKВ) за експериментального генітального герпесу в мурчаків, індукованого вірусом простого герпесу 2-го типу (ВПГ-2). Матеріали і методи. Генітальну герпетичну інфекцію моделювали за допомогою ВПГ-2 (штам ВН) у самиць безпородних мурчаків. Критеріями оцінки ступеня тяжкості інфекційного процесу були: площа і ступінь специфічних уражень, а також наявність набряку, гіперемії й виразок. У сироватці крові тварин визначали титр ВПГ-2 за допомогою загальноприйнятих вірусологічних методів дослідження. Спектр мікробіоти піхви тварин визначали за допомогою загальноприйнятих мікробіологічних методів дослідження. Ефективність дії пробіотичних бактерій оцінювали за максимального розвитку патологічного процесу: за згасанням клінічних проявів захворювання та інфекційного титру ТЦД ID50 ВПГ-2, а також скороченням термінів захворювання та індексом лікувальної дії (ІЛД). Результати досліджень та їх обговорення. Встановлено, що за експериментального генітального герпесу у мурчаків при застосуванні пробіотичного штаму L. caseі ІМВ В-7280 та композиції В-7280 – VKL – VKВ зменшувались: ступінь клінічних симптомів захворювання, його тривалість, а також інфекційний титр ВПГ-2, хоч ІЛД не перевищував 50 %. Під впливом L. caseі ІМВ В-7280 та композиції відбувалось зменшення інфекційного титру ТЦД ID50 ВПГ-2, що свідчить про антигерпетичну ефективність цих пробіотичних штамів бактерій, у механізми якої може залучатись зміна мікробіоти піхви. Мікробіологічний пейзаж піхви за максимальної яскравості клінічних симптомів генітального герпесу характеризувався зниженням кількості стафілококів у піхві інфікованих мурчаків, яким вводили L. caseі ІМВ В-7280, при високому рівні цих бактерій за умови застосування композиції В-7280 – VKL – VKВ, а також появою в окремих тварин лактобацил (ЛАБ). Зауважимо, що виділені нами в кінці експерименту ЛАБ за своєю морфологією відрізнялися від лактобактерій, використаних в експерименті. Тобто можна припустити, що відбулося відновлення характерної для мурчаків нормальної мікробіоти піхви. Висновок. Штам L. caseі ІМВ В-7280 та композиція В-7280 – VKL – VKВ є перспективними для розробки цільових пробіотиків для профілактики й лікування інфекційно-запальних хвороб сечостатевої системи.

Molecular discrimination of new isolates of Bifidobacterium animalis subsp. lactis from reference strains and commercial probiotic strains

2007 ◽  
Vol 17 (5) ◽  
pp. 565-573 ◽  
Author(s):  
Helmut K. Mayer ◽  
Ernst Amtmann ◽  
Elisabeth Philippi ◽  
Gudrun Steinegger ◽  
Sigrid Mayrhofer ◽  
...  
Keyword(s):  
Bifidobacterium Animalis ◽  
Probiotic Strains ◽  
Reference Strains

scholarly journals Tolerance and safety of Lactobacillus paracasei ssp. paracasei in combination with Bifidobacterium animalis ssp. lactis in a prebiotic-containing infant formula: a randomised controlled trial

2009 ◽  
Vol 102 (6) ◽  
pp. 869-875 ◽  
Author(s):  
Arine M. Vlieger ◽  
Afke Robroch ◽  
Stef van Buuren ◽  
Jeroen Kiers ◽  
Ger Rijkers ◽  
...  
Keyword(s):  
Infant Formula ◽  
Controlled Trial ◽  
Normal Growth ◽  
Antibiotic Use ◽  
Early Infancy ◽  
Lactobacillus Paracasei ◽  
Control Group ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains ◽  
Randomised Controlled

The addition of probiotics to infant formula has been shown to be an efficient way to increase the number of beneficial bacteria in the intestine in order to promote a gut flora resembling that of breast-fed infants. The objective of the present study was to evaluate the safety and tolerance of a combination of two probiotic strains in early infancy. A group of 126 newborns were randomised to receive a prebiotic-containing starter formula supplemented with Lactobacillus paracasei ssp. paracasei and Bifidobacterium animalis ssp. lactis or the same formula without probiotics for the first 3 months of life. A total of eighty infants continued the study until they were aged 6 months. Growth measurements were taken monthly at healthy baby clinics. Diaries were used to monitor behaviour, infections, use of antibiotics, as well as stool characteristics. Normal growth occurred in all infants and no statistically significant differences were detected between the probiotics group and the control group for gain in weight, length and head circumference. Infants in the probiotics group produced softer and more frequent stools during the first 3 months of life. No differences were found in crying and sleeping hours, number of parent-diagnosed infections, antibiotic use, visits to the general practitioner and number of adverse events. The use of a prebiotic-containing starter formula supplemented with L. paracasei ssp. paracasei and B. animalis ssp. lactis in early infancy is safe, well tolerated and has no adverse effects on growth and infant behaviour.

scholarly journals Study of Probiotic Effects of Bifidobacterium animalis subsp. lactis BB-12 and Lactobacillus plantarum 299v Strains on Biochemical and Morphometric Parameters of Rabbits after Obesity Induction

Biology ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 131
Author(s):  
Assia Bouaziz ◽  
Amira Leila Dib ◽  
Nedjoua Lakhdara ◽  
Louiza Kadja ◽  
Elena Espigares ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Lactobacillus Plantarum ◽  
High Fat Diet ◽  
Clinical Manifestations ◽  
Morphometric Parameters ◽  
Dependent Manner ◽  
Bifidobacterium Animalis ◽  
The Metabolic Syndrome ◽  
Probiotic Strains ◽  
Treatment Of Obesity

This study aimed first to develop an experimental model of obesity and metabolic syndrome over 14 weeks using a diet called “cafeteria”, which is a high-fat diet, to evaluate its consequences on the biochemical and morphometric parameters in ITELV2006 strain rabbits. Second, the trial aimed to evaluate the effect of two strains of probiotics, these being Bifidobacterium animalis subsp. lactis BB-12® and Lactobacillus plantarum 299v®, on the obesity and MetS induced during the first experiment. Overall, the results of the “cafeteria” diet demonstrated significant changes in numerous biochemical and morphometric parameters, reproducing obesity and the main clinical manifestations of the metabolic syndrome in humans. The administration of the two probiotic strains demonstrated an impact on certain parameters of obesity and induced MetS. This study makes it possible to conclude that probiotics could be useful in the treatment of obesity and metabolic syndrome of rabbits, but in a dependent manner. Furthermore, this study evidenced the importance of selecting specific probiotic strains and dosages to achieve desirable results on rabbits or other species.

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