Pioglitazone, an Activator of Peroxisome Proliferator-Activated Receptor (PPAR-γ), Reduces the Expression of KB Nuclear Transcription Factor and Inhibits Apoptosis in Peripheral Blood Mononuclear Cells in Vitro

2012 ◽  
Vol 3 (4) ◽  
pp. 371-377
Author(s):  
N. I. Kutsenko ◽  
L. E. Vesnina ◽  
Igor P. Kaidashev
Keyword(s):  
Transcription Factor ◽  
Peripheral Blood Mononuclear Cells ◽  
Mononuclear Cells ◽  
Peroxisome Proliferator ◽  
Peripheral Blood Mononuclear ◽  
Peroxisome Proliferator Activated Receptor ◽  
Nuclear Transcription Factor ◽  
Ppar Γ ◽  
Blood Mononuclear Cells

Evaluation of the action of LPSF/GQ-16 on cytokines and PPAR-γ gene expression after in vitro irradiation of peripheral blood mononuclear cells

2021 ◽  
pp. 1-22
Author(s):  
Mariana Brayner Cavalcanti ◽  
Lídia Lúcia Bezerra Leite ◽  
Diogo Manoel de Queiroz ◽  
Thiago de Salazar e Fernandes ◽  
Wagner Luís Mendes de Oliveira ◽  
...  
Keyword(s):  
Gene Expression ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Peripheral Blood Mononuclear ◽  
Ppar Γ ◽  
Blood Mononuclear Cells

scholarly journals MAR1 suppresses inflammatory response in LPS-induced RAW 264.7 macrophages and human primary peripheral blood mononuclear cells via the SIRT1/PGC-1α/PPAR-γ pathway

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Wei Wang ◽  
Rong-Li Xu ◽  
Ping He ◽  
Rui Chen
Keyword(s):  
Inflammatory Response ◽  
Mononuclear Cells ◽  
Raw 264.7 ◽  
Peroxisome Proliferator ◽  
Peripheral Blood Mononuclear ◽  
Peroxisome Proliferator Activated Receptor ◽  
Raw 264.7 Macrophages ◽  
Ppar Γ ◽  
Tnf Α ◽  
Blood Mononuclear Cells

Abstract Background Sepsis is a complex syndrome characterized by a dysregulated inflammatory response to systemic infection and leads to shock, multiple organ failure and death especially if not recognized early and treated promptly. Previous studies have suggested Maresin 1 (MAR1) can alleviate systemic inflammation in sepsis, but its mechanism has not been clarified. Methods RAW 264.7 cells and human primary peripheral blood mononuclear cells (hPBMCs) were pretreated with LPS and MAR1. The mRNA expression and supernatant levels of pro-inflammatory cytokines, tumor necrosis factor (TNF-α), interleukin (IL)-1β and IL-6 were evaluated by RT-qPCR and ELISA, respectively. The expression levels of Sirtuin 1 (SIRT1), peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), and Peroxisome proliferator-activated receptor gamma (PPAR-γ) were determined by RT-qPCR and Western blot analysis, respectively. Results Our results show that LPS-induced inflammation increased the expression and secretion of proinflammatory cytokines TNF-α, IL-1β and IL-6 and induced suppression of SIRT1, PGC-1α, and PPAR-γ expression, which could be reversed by MAR1. And the effect of MAR1 was eliminated by repression of SIRT1/PPAR-γ and enhanced by PGC-1α overexpression. Conclusions MAR1 suppressed inflammatory response in LPS-induced RAW 264.7 macrophages and hPBMCs via the SIRT1/PGC-1α/PPAR-γ pathway.

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