Changes in Arginase-Nitric Oxide Synthase System at Adaptation to Prolonged Exercise Training by Swimming in Adult and Aged Rat Hearts

2012 ◽  
Vol 3 (4) ◽  
pp. 299-308 ◽  
Author(s):  
Anatolii V. Kotsuruba ◽  
Yulia P. Korkach ◽  
Sergey O. Talanov ◽  
Olga V. Bazilyuk ◽  
Lyubov G. Stepanenko ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Exercise Training ◽  
Prolonged Exercise ◽  
Aged Rat ◽  
Rat Hearts ◽  
Oxide Synthase

Exercise training improves myocardial tolerance to ischemia in male but not in female rats

2007 ◽  
Vol 293 (1) ◽  
pp. R363-R371 ◽  
Author(s):  
David B. Thorp ◽  
James V. Haist ◽  
Jennifer Leppard ◽  
Kevin J. Milne ◽  
Morris Karmazyn ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Exercise Training ◽  
Endothelial Nitric Oxide Synthase ◽  
Diastolic Pressure ◽  
Female Rats ◽  
Female Rat ◽  
Rat Hearts ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Acute exercise increases myocardial tolerance to ischemia-reperfusion (I-R) injury in male but not in female rat hearts, possibly due to a decreased heat shock protein 70 (Hsp70) response in the female hearts. This study examined whether repetitive exercise training would increase Hsp70 and myocardial tolerance to I-R injury in female rat hearts. Adaptations in myocardial manganese superoxide dismutase (MnSOD) and endothelial nitric oxide synthase (eNOS) were also assessed. Ten-week old male (M) and female (F) Sprague-Dawley rats ( n = 40 total) exercise-trained for 14 wk; the last 8 wk consisted of running 1 h at 30 m/min (2% incline), 5 days/wk. Following training, left ventricle mechanical function (LVMF) was monitored for 30 min of reperfusion following 30 min of global ischemia (Langendorff procedure). Myocardial Hsp70 content was not different in M and F control groups, while increases were observed in both trained groups (M greater than F; P < 0.05). Although MnSOD content did not differ between groups, endothelial nitric oxide synthase (eNOS) levels were decreased in F, with no change in M, following training ( P < 0.05). Hearts from control F demonstrated a greater recuperation of all indices of LVMF following I-R compared with control M hearts ( P < 0.05). Hearts of trained M exhibited improved recovery of LVMF (left ventricular diastolic pressure, left ventrcular end-diastolic pressure, +dP/d t, −dP/d t) during reperfusion compared with control M hearts ( P < 0.05). In contrast, hearts of trained F did not show any change in recovery from I-R. Hence, exercise training is more beneficial to M than F in improving myocardial function following I-R injury.

Inducible Nitric Oxide Synthase-Activated Mitochondrial Apoptotic Pathway in Hypoxic and Aged Rat Hearts

Gerontology ◽  
2010 ◽  
Vol 56 (6) ◽  
pp. 544-552 ◽  
Author(s):  
S. Zara ◽  
M. Rapino ◽  
L. Centurione ◽  
V. di Giacomo ◽  
G. Petruccelli ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Apoptotic Pathway ◽  
Mitochondrial Apoptotic Pathway ◽  
Aged Rat ◽  
Rat Hearts ◽  
Oxide Synthase ◽  
Inducible Nitric Oxide

scholarly journals Arginase-nitric oxide synthase system changes due to adaptation to swimming in adult and aged rat hearts

2012 ◽  
Vol 58 (1) ◽  
pp. 27-35
Author(s):  
AV Kotsiuruba ◽  
◽  
IuP Korkach ◽  
SO Talanov ◽  
OV Basiliuk ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Aged Rat ◽  
Rat Hearts ◽  
System Changes ◽  
Oxide Synthase

scholarly journals Contractile responsiveness of coronary arteries from exercise-trained rats

1997 ◽  
Vol 83 (2) ◽  
pp. 434-443 ◽  
Author(s):  
Janet L. Parker ◽  
Mildred L. Mattox ◽  
M. Harold Laughlin
Keyword(s):  
Nitric Oxide ◽  
Coronary Artery ◽  
Nitric Oxide Synthase ◽  
Exercise Training ◽  
Sodium Nitroprusside ◽  
Coronary Arteries ◽  
Internal Diameter ◽  
Relaxation Responses ◽  
Proximal Coronary Artery ◽  
Oxide Synthase

Parker, Janet L., Mildred L. Mattox, and M. Harold Laughlin.Contractile responsiveness of coronary arteries from exercise trained rats. J. Appl. Physiol. 83(2): 434–443, 1997.—The purpose of this study was to determine whether exercise training alters vasomotor reactivity of rat coronary arteries. In vitro isometric microvessel techniques were used to evaluate vasomotor properties of proximal left anterior artery rings (1 ring per animal) from exercise-trained rats (ET; n = 10) subjected to a 12-wk treadmill training protocol (32 m/min, 15% incline, 1 h/day, 5 days/wk) and control rats (C; n = 6) restricted to cage activity. No differences in passive length-tension characteristics or internal diameter (158 ± 9 and 166 ± 9 μm) were observed between vessesls of C and ET rats. Concentration-response curves to K+ (5–100 mM), prostaglandin F2α(10−8–10−4M), and norepinephrine (10−8–10−4) were unaltered ( P > 0.05) in coronary rings from ET rats compared with C rats; however, lower values of the concentration producing 50% of the maximal contractile response in rings from ET rats ( P = 0.05) suggest that contractile sensitivity to norepinephrine was enhanced. Vasorelaxation responses to sodium nitroprusside (10−9-10−4M) and adenosine (10−9-10−4M) were not different ( P > 0.05) between vessels of C and ET rats. However, relaxation responses to the endothelium-dependent vasodilator acetylcholine (ACh; 10−10-10−4M) were significantly blunted ( P < 0.001) in coronary rings from ET animals; maximal ACh relaxation averaged 90 ± 5 and 46 ± 12%, respectively, in vessels of C and ET groups. In additional experiments, two coronary rings (proximal and distal) were isolated from each C ( n = 7) and ET ( n = 7) animal. Proximal coronary artery rings from ET animals demonstrated decreased relaxation responses to ACh; however, ACh-mediated relaxation of distal coronary rings was not different between C and ET groups. N G-monomethyl-l-arginine (inhibitor of nitric oxide synthase) blocked ACh relaxation of all rings. l-Arginine (substrate for nitric oxide synthase) did not improve the blunted ACh relaxation in proximal coronary artery rings from ET rats. These studies suggest that exercise-training selectively decreases endothelium-dependent (ACh) but not endothelium-independent (sodium nitroprusside) relaxation responses of rat proximal coronary arteries; endothelium-dependent relaxation of distal coronary arteries is unaltered by training.

Regulation of inducible nitric oxide synthase activity/expression in rat hearts from ghrelin-treated rats

2010 ◽  
Vol 67 (2) ◽  
pp. 195-204 ◽  
Author(s):  
Emina Sudar ◽  
Branislava Dobutovic ◽  
Sanja Soskic ◽  
Vesna Mandusic ◽  
Zorica Zakula ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Inducible Nitric Oxide Synthase ◽  
Rat Hearts ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity ◽  
Inducible Nitric Oxide

scholarly journals Exercise training enhances multiple mechanisms of relaxation in coronary arteries from ischemic hearts

2013 ◽  
Vol 305 (9) ◽  
pp. H1321-H1331 ◽  
Author(s):  
Rachel R. Deer ◽  
Cristine L. Heaps
Keyword(s):  
Nitric Oxide ◽  
Blood Flow ◽  
Coronary Artery ◽  
Nitric Oxide Synthase ◽  
Exercise Training ◽  
Coronary Arteries ◽  
Vascular Function ◽  
Channel Inhibition ◽  
Oxide Synthase ◽  
Endothelium Dependent Relaxation

Exercise training of coronary artery disease patients is of considerable interest, since it has been shown to improve vascular function and, thereby, enhance blood flow into compromised myocardial regions. However, the mechanisms underlying exercise-induced improvements in vascular function have not been fully elucidated. We tested the hypothesis that exercise training increases the contribution of multiple mediators to endothelium-dependent relaxation of coronary arteries in the underlying setting of chronic coronary artery occlusion. To induce gradual occlusion, an ameroid constrictor was placed around the proximal left circumflex coronary artery in Yucatan miniature swine. At 8 wk postoperatively, pigs were randomly assigned to sedentary or exercise (treadmill, 5 days/wk) regimens for 14 wk. Exercise training significantly enhanced the contribution of nitric oxide, prostanoids, and large-conductance Ca2+-dependent K+ (BKCa) channels to endothelium-dependent, bradykinin-mediated relaxation in nonoccluded and collateral-dependent arteries. Combined nitric oxide synthase, prostanoid, and BKCa channel inhibition ablated the enhanced relaxation associated with exercise training. Exercise training significantly increased nitric oxide levels in response to bradykinin in endothelial cells isolated from nonoccluded and collateral-dependent arteries. Bradykinin treatment significantly increased PGI2 levels in all artery treatment groups and tended to be further enhanced after nitric oxide synthase inhibition in exercise-trained pigs. No differences were found in whole cell BKCa channel currents, BKCa channel protein levels, or arterial cyclic nucleotide levels. Although redundant, upregulation of parallel vasodilator pathways appears to contribute to enhanced endothelium-dependent relaxation, potentially providing a more refined control of blood flow after exercise training.

Immunohistochemical study on the distribution of nitrotyrosine and neuronal nitric oxide synthase in aged rat cerebellum

2002 ◽  
Vol 951 (2) ◽  
pp. 316-321 ◽  
Author(s):  
Yoon Hee Chung ◽  
Chung Min Shin ◽  
Kyeung Min Joo ◽  
Myeung Ju Kim ◽  
Choong Ik Cha
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Immunohistochemical Study ◽  
Neuronal Nitric Oxide Synthase ◽  
Aged Rat ◽  
Rat Cerebellum ◽  
Oxide Synthase
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