The Analysis of the Genetic Parameters of Chlorella vulgaris Beyer. Culture Growing in the Presence of Sodium Selenite, Zinc Sulfate and Chromium Chloride

2021 ◽  
Vol 23 (3) ◽  
pp. 257-268
Author(s):  
O. I. Bodnar ◽  
I. O. Andreev ◽  
M. Z. Prokopiak ◽  
N. M. Drobyk ◽  
V. V. Grubinko
Keyword(s):  
Chlorella Vulgaris ◽  
Zinc Sulfate ◽  
Sodium Selenite ◽  
Genetic Parameters ◽  
Chromium Chloride

scholarly journals Effects of selenium-zinc-lipid substances from Chlorella vulgaris Biej. on oxidative and tnergy status of rats

2015 ◽  
Vol 17 (4) ◽  
Author(s):  
H. B. Vinyarska ◽  
P. H. Lykhatskyi ◽  
O. I. Bodnar ◽  
L. S. Fira ◽  
V. V. Grubinko
Keyword(s):  
Blood Serum ◽  
Chlorella Vulgaris ◽  
Oxidase Activity ◽  
Zinc Sulfate ◽  
Antioxidant Status ◽  
Sodium Selenite ◽  
Active Components ◽  
Experimental Animals ◽  
Unicellular Algae ◽  
Water Suspensions

<p>There were obtained and allocated stable selen-lipid and selen-zinc-lipid substances in a result of incubationof unicellular algae Chlorella vulgaris Biej. In aquaculture with sodium selenite and zinc sulfate their influence on oxidative and energetic status of healthy rat was investigated. There were suppressed prooxidative processes,activated antioxidant status, succinate dehydrogenase and cytochrome oxidase activity and glutamate dehydrogenase way of formation of glutamate were increased when substance at a dose of 0.4 mcg of selenium, 2.5 mcg of zinc<br />and 0.5 mg of lipid per 1 ml of 1 % starch-water suspensions was introduced in organisme of healthy rats. Also it contributed of formation and functioning of the active components of the antioxidant system – catalase, ceruloplasmin,<br />reduction glutathione in a liver and in a blood serum of experimental animals.</p>

scholarly journals Regulation of biosynthesis of lipids in Chlorella vulgaris by compounds of zinc, chromium and selenium

2018 ◽  
Vol 9 (2) ◽  
pp. 267-274
Author(s):  
O. I. Bodnar ◽  
H. B. Kovalska ◽  
V. V. Grubinko
Keyword(s):  
Fatty Acids ◽  
Chlorella Vulgaris ◽  
Sodium Selenite ◽  
Total Content ◽  
Biologically Active ◽  
Control Group ◽  
General Tendency ◽  
Zinc Ions ◽  
Regulation Of Biosynthesis ◽  
Trace Elemental

We studied molecular and metabolic mechanisms of regulated lipid biosynthesis in Chlorella vulgaris aquaculture. after addition of sodium selenite (10 mg/dm3) when added separately and in combination with Zn2+ (5 mg/dm3) and Cr3+ (5 mg/dm3) during 7 days of their action in order to obtain biotechnologically useful lipid products, enriched with microelements. Experiments were carried out in accordance with generally accepted hydrological and biochemical methods. It was established that micronutrients that were added into the medium result in an increase in the total content of lipids in the range of 10%. The redeployment of lipid classes in chlorella cells occurs due to the action of sodium selenite in favour of phospholipids by reducing the proportion of diacylglycerols, while the amount of triacylglycerols and nonetherified fatty acids does not change. Combined action of sodium selenite and zinc ions leads to the significant increase of the relative content of diacylglycerols, and partial increase of nonetherified fatty acids, at the same time in the cells we can observe a slight decrease in the proportion of triacylglycerols and phospholipids. Inclusion of 14C-bicarbonate in carbohydrates, proteins and lipids of Ch. vulgaris is significantly different both from the control group and from the group to which we added the investigated factors. However, the predominance of inclusion in lipids is 2–3 times higher than its inclusion into carbohydrates and 9–12 times higher in proteins. The increase of labeled bicarbonate inclusion intensity into carbohydrates occurs only in the case of joint action of sodium selenite and zinc ions, in proteins and lipids – in all cases of trace elemental activity. It was revealed that the general tendency is the reduction of the inclusion of bicarbonate in Ch. vulgaris triacylglycerols and its increase in phospholipids and nonetherified fatty acids, except for chromium ions, that modified the inclusion of the label into diacylglycerols, which may be due to the specific toxicity of the metal ions. The activation of lipogenesis after addition of selenium, zinc and chromium compounds was confirmed by an increase in the inclusion intensity of 14C-oleate in various classes of lipids that are present in chlorella and increased activity of glycerol-3-phosphatacyltransferase. Direction and regulation of lipid metabolism in Ch. vulgaris in the direction of increasing the amount and accumulation of lipids and their separate classes using sodium selenite in combination with Zn2+ and Cr3+ with the purpose of forming selenium-metal-lipid complexes can be used to obtain biologically active lipidous preparations enriched with essential microelements.

scholarly journals EFFECT OF SELENIUM-CHROME-LIPID SUBSTANCE FROM CHLORELLA VULGARIS BIEJ. ON OXIDATIVE STATUS OF RATS

2016 ◽  
Author(s):  
O. Ya. Lukashiv ◽  
O. I. Bodnar ◽  
H. B. Vinyarskа ◽  
V. V. Hrubinko
Keyword(s):  
Blood Serum ◽  
Chlorella Vulgaris ◽  
Antioxidant Status ◽  
Sodium Selenite ◽  
Oxidative Status ◽  
Unicellular Alga ◽  
Experimental Animals ◽  
Chrome Iii

By incubation unicellular alga Chlorella vulgaris Biej. in aquaculture with sodium selenite and chrome (III) sulphate there was received and allocated stable lipid and selenium, chrome-lipid substance and studied their effects on oxidative status in healthy rats experiment. Putting substances at a dose of 1.85 mсg of selenium, chrome 1.1 mcg and 0.5 mg lipid per 1 ml of 1 % aqueous starch slurry body in healthy rats every day for 14 days suppressed prooxidative processes activated antioxidant status by glutation system by reduction of catalase and superoxidedismutase activity partly in experimental animals in the liver and in blood serum.

scholarly journals EFFECTS OF SELENIUM, IRON AND ZINC SUPPLEMENTATION ON ARSENIC CONCENTRATION IN TISSUES WITH GROSS PATHOLOGY IN LONG EVANS RATS

2012 ◽  
Vol 3 (1) ◽  
pp. 83-85 ◽  
Author(s):  
MN Islam ◽  
MA Awal ◽  
SML Kabir ◽  
MS Islam ◽  
MW Islam
Keyword(s):  
Arsenic Trioxide ◽  
Ferrous Sulfate ◽  
Zinc Sulfate ◽  
Sodium Selenite ◽  
Zinc Supplementation ◽  
Arsenic Concentration ◽  
Male Rats ◽  
Gross Pathology ◽  
Iron And Zinc ◽  
Long Evans

The study was carried out to know the effects of selenium, iron and zinc supplementation on gross pathology and tissue arsenic concentration in Long Evans rats during the period from August to October 2003. A total of 25, one-month-old male rats were randomly divided into 5 equal groups as A, B, C, D and E. Rats of group A were kept as control without giving any treatment. Rats of groups B, C and D were given normal rat pellet (25 g / rat / day) and in addition to arsenic trioxide (As2O3) @ 400 mg / litre, they were treated with sodium selenite (NaSe) @ 1 mg / litre, ferrous sulfate (FeSO4) @ 150 mg / litre and zinc sulfate (ZnSO4) @ 50 mg / litre respectively while rats of group E were treated with only arsenic trioxide (AS2O3) 400 @ mg / litre drinking water daily for 42 days. All the rats of groups B, C and D showed slight congestion in the liver, spleen, heart and kidney and haemorrhage in stomach and intestine but the rats of group E showed heavy congestion in the liver, spleen, heart and kidney and rose-red inflammation in the stomach and severe haemorrhagic enteritis. Among the treated groups, the rats of groups B (0.0126-0.27 ppm), C (0.03-0.32 ppm) and D (0.04-0.288 ppm) which were supplemented with sodium selenite, ferrous sulfate and zinc sulfate respectively along with arsenic trioxide showed reduced arsenic concentrations in different tissues while the rats of group E treated only with arsenic trioxide showed higher concentrations of arsenic in tissues (0.05-0.4 ppm). The concentration of arsenic in the liver, kidney, spleen, heart, stomach, intestine, muscle and dermis of the treated rats were 0.27-0.40 ppm, 0.06-0.22 ppm, 0.052-0.20 ppm, 0.0126-0.102 ppm, 0.054-0.16 ppm, 0.064-0.176 ppm, 0.028-0.07 ppm and 0.03-0.05 ppm respectively. It may be concluded that supplementation of selenium, iron and zinc reduces the arsenic concentration in tissues like liver, kidney, spleen, heart, intestine, stomach, muscle and dermis of rats as well as lower the tissue damage caused by arsenic. The present results also indicate that arsenic is deposited mainly in liver followed by kidney, spleen, dermis, heart and muscle.

Protective effect of sodium selenite and zinc sulfate on intensive swimming-induced testicular gamatogenic and steroidogenic disorders in mature male rats

2008 ◽  
Vol 33 (5) ◽  
pp. 903-914 ◽  
Author(s):  
Kuladip Jana ◽  
Pravat K. Samanta ◽  
Indranil Manna ◽  
Prasanta Ghosh ◽  
Narendra Singh ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Zinc Sulfate ◽  
Sodium Selenite ◽  
Sperm Count ◽  
Plasma Concentrations ◽  
Plasma Corticosterone ◽  
Seminiferous Epithelium ◽  
Male Rats ◽  
Control Group ◽  
Control Groups

To investigate the ameliorative potential of sodium selenite and zinc sulfate on intensive-swimming-induced testicular disorders, 48 Wistar male rats (age, 4 months; mass, 146.2 ± 3.6 g) were randomly divided into 4 groups: the unexercised-control group (n = 12); the exercised group (n = 12); the control supplemented group (n = 12); and the exercised supplemented group (n = 12). For 10 weeks, the exercised rats underwent a protocol that consisted of 4 h·d–1swimming, for 6 d·week–1; the control rats did not exercise. For 10 weeks, both the supplemented groups received an oral daily dose of a combination of sodium selenite and zinc sulfate (6  and 3 mg·kg body mass–1, respectively). After 10 weeks, a significant reduction (p < 0.05) was seen in rats in the exercised group, compared with rats in both control groups, in paired testicular masses; in epididymal sperm count; in testicular Δ5, 3β-hydroxysteroid dehydrogenase (HSD) and 17β-HSD; in plasma levels of testosterone, luteinizing hormone, follicle-stimulating hormone, and prolactin; in the numbers of preleptotine spermatocytes, midpachytene spermatocytes, and stage 7 spermatids of the stage VII seminiferous epithelium cycle; and in fertility performance. As well, a significant increase (p < 0.05) was seen in the exercised group, compared with both control groups, in plasma corticosterone levels and in testicular content of malondialdehyde and catalase activity. At the same time, there was a significant reduction (p < 0.05) in the exercised group, compared with both control groups, in plasma concentrations of zinc and selenium; in the testicular content of glutathione (GSH), the glutathione and glutathione disulphide (GSSG) ratio, ascorbic acid, and α-tocopherol; and in testicular activities of superoxide dismutase, glutathione-peroxidase, and glutathione-S-transferase in the testes. No significant changes were seen in the number of spermatogonia-A from the stage VII seminiferous epithelium cycle or the testicular content of GSSG among the groups. Sodium selenite and zinc sulfate supplementation significantly protected against exercise-induced testicular gamatogenic and spermatogenic disorders, prevented testicular oxidative stress, and increased antioxidant status. It can be concluded that intensive-swimming-induced oxidative stress causes dysfunctions in the male reproductive system, which can be protected by the coadministration of sodium selenite and zinc sulfate.

The Zinc Sulfate Turbidity Test

1957 ◽  
Vol 32 (3) ◽  
pp. 483-493 ◽  
Author(s):  
Thomas E. Wilson ◽  
Charles H. Brown ◽  
Adrian Hainline
Keyword(s):  
Zinc Sulfate ◽  
Turbidity Test

Feasible design for electricity generation from Chlorella vulgaris using convenient photosynthetic conditions

BIOCELL ◽  
2018 ◽  
Vol 42 (1) ◽  
pp. 7-11 ◽  
Author(s):  
M. Moustafa ◽  
T. Taha ◽  
M. Elnouby ◽  
M.A. Abu-Saied Aied ◽  
A. Shati ◽  
...  
Keyword(s):  
Chlorella Vulgaris ◽  
Electricity Generation
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