Ultrastructural effects of heavy metals on the epidermal cells of Dilophus spiralis (Mont) Hamel (Dictyotales, Phaeophyta)

B.-E. Diannelidis; S. G. Delivopoulos

doi:10.1615/interjalgae.v1.i3.20

Ultrastructural effects of heavy metals on the epidermal cells of Dilophus spiralis (Mont) Hamel (Dictyotales, Phaeophyta)

International Journal on Algae ◽

10.1615/interjalgae.v1.i3.20 ◽

1999 ◽

Vol 1 (3) ◽

pp. 21-30

Author(s):

B.-E. Diannelidis ◽

S. G. Delivopoulos

Keyword(s):

Heavy Metals ◽

Epidermal Cells

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Gating of aquaporins by heavy metals in Allium cepa L. epidermal cells

PROTOPLASMA ◽

10.1007/s00709-010-0222-9 ◽

2010 ◽

Vol 248 (4) ◽

pp. 663-671 ◽

Cited By ~ 38

Author(s):

Ewa Maria Przedpelska-Wasowicz ◽

Malgorzata Wierzbicka

Keyword(s):

Heavy Metals ◽

Allium Cepa ◽

Epidermal Cells ◽

Allium Cepa L

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Sampling and analysis of the air-water interface with SEM and EDS of microlayers

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100157000 ◽

1989 ◽

Vol 47 ◽

pp. 1004-1005

Author(s):

Randall W. Smith ◽

John Dash

Keyword(s):

Heavy Metals ◽

Surface Microlayer ◽

Surface Films ◽

Water Interface ◽

Physical Processes ◽

Infrared Spectroscopic Analysis ◽

Eds Analysis ◽

Air Water Interface ◽

Significant Area ◽

Bulk Chemical

The structure of the air-water interface forms a boundary layer that involves biological ,chemical geological and physical processes in its formation. Freshwater and sea surface microlayers form at the air-water interface and include a diverse assemblage of organic matter, detritus, microorganisms, plankton and heavy metals. The sampling of microlayers and the examination of components is presently a significant area of study because of the input of anthropogenic materials and their accumulation at the air-water interface. The neustonic organisms present in this environment may be sensitive to the toxic components of these inputs. Hardy reports that over 20 different methods have been developed for sampling of microlayers, primarily for bulk chemical analysis. We report here the examination of microlayer films for the documentation of structure and composition.Baier and Gucinski reported the use of Langmuir-Blogett films obtained on germanium prisms for infrared spectroscopic analysis (IR-ATR) of components. The sampling of microlayers has been done by collecting fi1ms on glass plates and teflon drums, We found that microlayers could be collected on 11 mm glass cover slips by pulling a Langmuir-Blogett film from a surface microlayer. Comparative collections were made on methylcel1ulose filter pads. The films could be air-dried or preserved in Lugol's Iodine Several slicks or surface films were sampled in September, 1987 in Chesapeake Bay, Maryland and in August, 1988 in Sequim Bay, Washington, For glass coverslips the films were air-dried, mounted on SEM pegs, ringed with colloidal silver, and sputter coated with Au-Pd, The Langmuir-Blogett film technique maintained the structure of the microlayer intact for examination, SEM observation and EDS analysis were then used to determine organisms and relative concentrations of heavy metals, using a Link AN 10000 EDS system with an ISI SS40 SEM unit. Typical heavy microlayer films are shown in Figure 3.

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