A numerical and experimental study on the vacuum freeze-drying process of skim milk solution in a vial

2002 ◽  
Author(s):  
Chi Sung Song ◽  
Jin Hyun Nam ◽  
Charn Jung Kim ◽  
Sung Tack Ro
Keyword(s):  
Experimental Study ◽  
Freeze Drying ◽  
Skim Milk ◽  
Drying Process

scholarly journals Study and optimization of freeze-drying cycles of a model probiotic strain

2018 ◽  
Author(s):  
Pierre Verlhac ◽  
Séverine Vessot-Crastes ◽  
Ghania Degobert ◽  
Claudia Cogné ◽  
Julien Andrieu ◽  
...  
Keyword(s):  
Experimental Study ◽  
Survival Rate ◽  
Lactobacillus Casei ◽  
Freeze Drying ◽  
Survival Rates ◽  
Probiotic Strain ◽  
Porous Matrix ◽  
Drying Process ◽  
The Matrix ◽  
The Impact

This work is based on the experimental study of the freeze-drying process to understand the impact of numerous factors on the survival rates of a model probiotic strain of Lactobacillus casei type. With the aim to find out if cell density in the matrix and survival rates are linked, we have studied the location of the cells after freeze drying inside a porous  matrix composed of a lactose basis with a polymer, the polyvinylpyrrolidone (PVP) in various amounts. The best survival rate were obtained at slow freezing rate for a formulation containing 5% (m/V) of lactose and 5% (m/V) of PVP. Keywords: Freeze-Drying; Freezing; Probiotics; L. Casei ATCC 393 

scholarly journals Viability of Bifidobacterium Pseudocatenulatum G4 after Spray-Drying and Freeze-Drying

2010 ◽  
Vol 3 ◽  
pp. MBI.S2728 ◽  
Author(s):  
Stephenie Wong ◽  
Barka Mohammed Kabeir ◽  
Shuhaimi Mustafa ◽  
Rosfarizan Mohamad ◽  
Anis Shobirin Meor Hussin ◽  
...  
Keyword(s):  
Spray Drying ◽  
Freeze Drying ◽  
Skim Milk ◽  
The Other ◽  
Outlet Temperature ◽  
Residual Moisture Content ◽  
Drying Process ◽  
Residual Moisture ◽  
Heat Adaptation ◽  
C 30

Viability of Bifidobacterium pseudocatenulatum G4 following spray-drying and freeze-drying in skim milk was evaluated. After spray-drying, the strain experienced over 99% loss in viability regardless of the air outlet temperature (75 and 85 °C) and the heat-adaptation temperature (45 and 65 °C, 30 min). The use of heat-adaptation treatment to improve the thermotolerance of this strain was ineffective. On the other hand, the strain showed a superior survival at 71.65%–82.07% after freeze-drying. Viable populations of 9.319–9.487 log10 cfu/g were obtained when different combinations of skim milk and sugar were used as cryoprotectant. However, the addition of sugars did not result in increased survival during the freeze-drying process. Hence, 10% (w/v) skim milk alone is recommended as a suitable protectant and drying medium for this strain. The residual moisture content obtained was 4.41% ± 0.44%.

scholarly journals An Experimental Study on the Freeze Drying Process for Poly γ Glutamic Acid

2015 ◽  
Vol 27 (12) ◽  
pp. 645-651 ◽  
Author(s):  
Jisu Kang ◽  
Yeon-Ho Sim ◽  
Si-Ye Byun ◽  
Young Soo Chang ◽  
Byung Ha Kang
Keyword(s):  
Experimental Study ◽  
Glutamic Acid ◽  
Freeze Drying ◽  
Drying Process

Optimization of the Freeze-Drying Media and Survival throughout Storage of Freeze-Dried Aerotolerant Bifidobacterium animailis Subsp. Lactis Qq08

2011 ◽  
Vol 140 ◽  
pp. 394-399
Author(s):  
Qing Qing Li ◽  
Juan Xu ◽  
Mei Ling Chen ◽  
Hui Ruan ◽  
Guo Qing He ◽  
...  
Keyword(s):  
Survival Rate ◽  
Freeze Drying ◽  
Skim Milk ◽  
Single Factor ◽  
Drying Process ◽  
Orthogonal Experiments ◽  
Average Survival ◽  
Freeze Dried ◽  
Average Survival Rate

The aim of the present work was to optimize the formula of cryoprotectants for freeze drying process of the previous selectedBifidobacteriumanimailissubsp.lactisQq08 (Bl Qq08). Single factor and orthogonal experiments were conducted to optimize the cryoprotectants formula. The formula of cryoprotectants was optimized as follows: skim milk 15%, lactose 8%, Vc 1.5% and glycerol 3%. In the triplicate lyophilization tests, the average survival rate of Bl Qq08 reached 91.23%.The optimized formula of cryoprotectants was suitable for freeze drying of the Bl Qq08.

Video real-time imaging of artificial membranes during freeze-drying by cryo-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 16-17
Author(s):  
Alan S. Rudolph ◽  
Ronald R. Price
Keyword(s):  
Real Time ◽  
Self Assembly ◽  
Freeze Drying ◽  
Video Capture ◽  
Drying Process ◽  
Artificial Membranes ◽  
The Past ◽  
Cryo Electron Microscopy ◽  
Spherical Structures ◽  
Capture Techniques

We have employed cryoelectron microscopy to visualize events that occur during the freeze-drying of artificial membranes by employing real time video capture techniques. Artificial membranes or liposomes which are spherical structures within internal aqueous space are stabilized by water which provides the driving force for spontaneous self-assembly of these structures. Previous assays of damage to these structures which are induced by freeze drying reveal that the two principal deleterious events that occur are 1) fusion of liposomes and 2) leakage of contents trapped within the liposome [1]. In the past the only way to access these events was to examine the liposomes following the dehydration event. This technique allows the event to be monitored in real time as the liposomes destabilize and as water is sublimed at cryo temperatures in the vacuum of the microscope. The method by which liposomes are compromised by freeze-drying are largely unknown. This technique has shown that cryo-protectants such as glycerol and carbohydrates are able to maintain liposomal structure throughout the drying process.

scholarly journals Strengthening of Porcine Plasma Protein Superabsorbent Materials through a Solubilization-Freeze-Drying Process

Polymers ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 772
Author(s):  
Estefanía Álvarez-Castillo ◽  
Carlos Bengoechea ◽  
Antonio Guerrero
Keyword(s):  
Water Uptake ◽  
Plasma Protein ◽  
Freeze Drying ◽  
Meat Industry ◽  
Drying Process ◽  
Uptake Capacity ◽  
Water Uptake Capacity ◽  
Protein Flour ◽  
Hydrophilic Materials ◽  
By Products

The replacement of common acrylic derivatives by biodegradable materials in the formulation of superabsorbent materials would lessen the associated environmental impact. Moreover, the use of by-products or biowastes from the food industry that are usually discarded would promote a desired circular economy. The present study deals with the development of superabsorbent materials based on a by-product from the meat industry, namely plasma protein, focusing on the effects of a freeze-drying stage before blending with glycerol and eventual injection molding. More specifically, this freeze-drying stage is carried out either directly on the protein flour or after its solubilization in deionized water (10% w/w). Superabsorbent materials obtained after this solubilization-freeze-drying process display higher Young’s modulus and tensile strength values, without affecting their water uptake capacity. As greater water uptake is commonly related to poorer mechanical properties, the proposed solubilization-freeze-drying process is a useful strategy for producing strengthened hydrophilic materials.

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