scholarly journals Evaluating Sampling Method Bias in Culex tarsalis and Culex quinquefasciatus (Diptera: Culicidae) Bloodmeal Identification Studies

2012 ◽  
Vol 49 (1) ◽  
pp. 143-149 ◽  
Author(s):  
Tara C. Thiemann ◽  
William K. Reisen
Keyword(s):  
Culex Quinquefasciatus ◽  
Sampling Method ◽  
Culex Tarsalis ◽  
Method Bias ◽  
Bloodmeal Identification

scholarly journals Infection, Dissemination, and Transmission Potential of North American Culex quinquefasciatus, Culex tarsalis, and Culicoides sonorensis for Oropouche Virus

Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 226
Author(s):  
Bethany L. McGregor ◽  
C. Roxanne Connelly ◽  
Joan L. Kenney
Keyword(s):  
Culex Quinquefasciatus ◽  
North American ◽  
Vector Competence ◽  
Febrile Illness ◽  
Culex Tarsalis ◽  
Biting Midges ◽  
Transmission Potential ◽  
High Infection ◽  
Culicoides Sonorensis ◽  
Vector Borne

Oropouche virus (OROV), a vector-borne Orthobunyavirus circulating in South and Central America, causes a febrile illness with high rates of morbidity but with no documented fatalities. Oropouche virus is transmitted by numerous vectors, including multiple genera of mosquitoes and Culicoides biting midges in South America. This study investigated the vector competence of three North American vectors, Culex tarsalis, Culex quinquefasciatus, and Culicoides sonorensis, for OROV. Cohorts of each species were fed an infectious blood meal containing 6.5 log10 PFU/mL OROV and incubated for 10 or 14 days. Culex tarsalis demonstrated infection (3.13%) but not dissemination or transmission potential at 10 days post infection (DPI). At 10 and 14 DPI, Cx. quinquefasciatus demonstrated 9.71% and 19.3% infection, 2.91% and 1.23% dissemination, and 0.97% and 0.82% transmission potential, respectively. Culicoides sonorensis demonstrated 86.63% infection, 83.14% dissemination, and 19.77% transmission potential at 14 DPI. Based on these data, Cx. tarsalis is unlikely to be a competent vector for OROV. Culex quinquefasciatus demonstrated infection, dissemination, and transmission potential, although at relatively low rates. Culicoides sonorensis demonstrated high infection and dissemination but may have a salivary gland barrier to the virus. These data have implications for the spread of OROV in the event of a North American introduction.

scholarly journals LABORATORY TRANSMISSION OF ST. LOUIS ENCEPHALITIS VIRUS BY THREE GENERA OF MOSQUITOES

1943 ◽  
Vol 78 (4) ◽  
pp. 241-253 ◽  
Author(s):  
W. McD. Hammon ◽  
W. C. Reeves
Keyword(s):  
Incubation Period ◽  
Culex Quinquefasciatus ◽  
Culex Pipiens ◽  
Culex Tarsalis ◽  
Subcutaneous Route ◽  
Aedes Vexans ◽  
Epidemiologic Evidence ◽  
Anopheles Maculipennis ◽  
Aedes Taeniorhynchus ◽  
Culex Stigmatosoma

1. St. Louis virus has been successfully transmitted in the laboratory by the following 9 species of mosquitoes from 3 genera: Culex tarsalis, Culex pipiens, Culex coronator, Aedes lateralis, Aedes taeniorhynchus, Aedes vexans, Aedes nigromaculis, Theobaldia incidens, and Theobaldia inornata. 2. Though transmission has not been demonstrated, survival of the virus for more than a few days was shown to occur in Culex quinquefasciatus, Culex stigmatosoma, Psorophora ciliata, and Anopheles maculipennis freeborni. 3. In experiments with Culex tarsalis, infection occurred from feeding on chickens and ducks which had been previously inoculated by the subcutaneous route. After an incubation period these mosquitoes infected other chickens and virus was in turn demonstrated in the blood of these. This is interpreted as proof that fowl may serve as reservoirs of virus in nature. Since mosquitoes have been repeatedly found naturally infected with St. Louis virus and epidemiologic evidence supports their incrimination, their rôle as vectors is now established. The fully incriminated species is Culex tarsalis.

scholarly journals Vector competence of Aedes aegypti, Culex tarsalis, and Culex quinquefasciatus from California for Zika virus

2018 ◽  
Vol 12 (6) ◽  
pp. e0006524 ◽  
Author(s):  
Bradley J. Main ◽  
Jay Nicholson ◽  
Olivia C. Winokur ◽  
Cody Steiner ◽  
Kasen K. Riemersma ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Culex Quinquefasciatus ◽  
Zika Virus ◽  
Vector Competence ◽  
Culex Tarsalis

scholarly journals Diversity and distribution bromeliads plants as breeding habitat for mosquito larvae (Diptera: Culicidae) in Bogor, Indonesia

2020 ◽  
Vol 21 (8) ◽  
Author(s):  
M Ikhsan ◽  
UPIK KESUMAWATI HADI ◽  
SUSI SOVIANA
Keyword(s):  
Aedes Albopictus ◽  
Culex Quinquefasciatus ◽  
Dominant Species ◽  
Sampling Method ◽  
Breeding Site ◽  
Breeding Habitat ◽  
Mosquito Larvae ◽  
Breeding Ground ◽  
Armigeres Subalbatus

Abstract. Ikhsan M, Hadi UK, Soviana S. 2020. Diversity and distribution bromeliads plants as breeding habitat for mosquito larvae (Diptera: Culicidae) in Bogor, Indonesia. Biodiversitas 21: 3494-3498. Bromeliads leaf structure can hold water and has potential as the breeding ground for mosquitoes. Information regarding the potential of bromeliads as a habitat for mosquito larvae in Indonesia is limited. This study aims to identify the diversity of bromeliads and their potential as breeding habitat for mosquito larvae in West Bogor Sub-district, Bogor City, West Java, Indonesia. Determination of the sample using purposive sampling method. Larvae collections were carried out on bromeliads that were outside the building in December 2019 to Maret 2020. The collection used a 50 ml syringe that was modified using a small pipe. Total bromeliads collected were 117 consisted of Neoregelia, Cryptanthus, dan Alcantarea from 50 observation points. The percentage of mosquito larvae presence from bromeliads was 74.4%. Total larvae collected were 859 larvae consisting of Aedes albopictus, Aedes aegypti, Aedes spp., Armigeres subalbatus, and Culex quinquefasciatus. The diversity of mosquito larvae found in the bromeliads were low (H'=0.16), while the dominant species of mosquito larvae were Ae. albopictus (61.55%) and confirmed as the second vector of Dengue Fever in Indonesia. The results of this study indicate that bromeliads have potential as a breeding site for mosquito larvae in West Bogor Sub-district, Bogor City.

scholarly journals Whole genome assembly of Culex tarsalis

2020 ◽  
Author(s):  
Bradley J. Main ◽  
Matteo Marcantonio ◽  
J. Spencer Johnston ◽  
Jason L. Rasgon ◽  
C. Titus Brown ◽  
...  
Keyword(s):  
Insecticide Resistance ◽  
Culex Quinquefasciatus ◽  
Reference Genome ◽  
Vector Competence ◽  
Mosquito Species ◽  
Single Copy ◽  
Specific Gene ◽  
Culex Tarsalis ◽  
Single Male ◽  
A Genome

AbstractThe mosquito, Culex tarsalis, is a key vector in the western United States due to its role in transmission of zoonotic arboviruses that affect human health. Extensive research has been conducted on Cx. tarsalis ecology, feeding behavior, vector competence, autogeny, diapause, genetics, and insecticide resistance. Population genetic analyses in the western U.S. have identified at least three genetic clusters that are geographically distinct. Salivary gland-specific gene expression has also revealed genes involved in blood feeding. However, genetic studies of this mosquito have been hindered by the lack of a reference genome. To facilitate genomic studies in Cx. tarsalis, we have assembled and annotated a reference genome (CtarK1) based on PacBio HiFi reads from a single male. Using the Cx. tarsalis transcriptome and protein sequences from Culex quinquefasciatus, approximately 17,456 protein-coding genes, including the para insecticide resistance gene, were annotated in the CtarK1 genome. Genome completeness was assessed using the Benchmarking Universal Single-Copy Orthologs (BUSCO) tool, which identified 84.8% of the 2799 Dipteran BUSCO genes. The CtarK1 assembly is 790Mb with an N50 of 58kb. Using full mitochondrial genome alignments with other sequenced mosquito genomes we present a Bayesian phylogeny, which estimates that the divergence of Cx. tarsalis from Culex quinquefasciatus, the most closely related mosquito species with a genome, occurred 15.8-22.2 million years ago.

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