Gene expression analysis and validation for potential marker genes of mycorrhizal rice plants in response to feeding with fall armyworm, Spodoptera frugiperda

2016 ◽  
Author(s):  
Lina Bernaola
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Spodoptera Frugiperda ◽  
Gene Expression Analysis ◽  
Fall Armyworm ◽  
Marker Genes ◽  
Rice Plants ◽  
Potential Marker

scholarly journals A Combinatory Approach for Selecting Prognostic Genes in Microarray Studies of Tumour Survivals

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Qihua Tan ◽  
Mads Thomassen ◽  
Kirsten M. Jochumsen ◽  
Ole Mogensen ◽  
Kaare Christensen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Feature Selection ◽  
Expression Analysis ◽  
Survival Data ◽  
Gene Expression Analysis ◽  
Cancer Survival ◽  
Feature Space ◽  
Differentially Expressed ◽  
Selection Strategy ◽  
Marker Genes

Different from significant gene expression analysis which looks for genes that are differentially regulated, feature selection in the microarray-based prognostic gene expression analysis aims at finding a subset of marker genes that are not only differentially expressed but also informative for prediction. Unfortunately feature selection in literature of microarray study is predominated by the simple heuristic univariate gene filter paradigm that selects differentially expressed genes according to their statistical significances. We introduce a combinatory feature selection strategy that integrates differential gene expression analysis with the Gram-Schmidt process to identify prognostic genes that are both statistically significant and highly informative for predicting tumour survival outcomes. Empirical application to leukemia and ovarian cancer survival data through-within- and cross-study validations shows that the feature space can be largely reduced while achieving improved testing performances.

scholarly journals Gene expression analysis using human cancer xenografts to identify novel predictive marker genes for the efficacy of 5-fluorouracil-based drugs

2006 ◽  
Vol 97 (6) ◽  
pp. 510-522 ◽  
Author(s):  
Akio Ooyama ◽  
Teiji Takechi ◽  
Etsuko Toda ◽  
Hideki Nagase ◽  
Yoshihiro Okayama ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Human Cancer ◽  
Predictive Marker ◽  
Marker Genes

scholarly journals Gene Expression Analysis of Chondrogenic Markers in Hair Follicle Dermal Papillae Cells Under the Effect of Laser Photobiomodulation and the Synovial Fluid

2019 ◽  
Vol 10 (3) ◽  
pp. 171-178
Author(s):  
Shirin Farivar ◽  
Roya Ramezankhani ◽  
Ezedin Mohajerani ◽  
Mohammad Hosein Ghazimoradi ◽  
Reza Shiari
Keyword(s):  
Gene Expression ◽  
Synovial Fluid ◽  
Hair Follicle ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Morphological Changes ◽  
Dermal Papilla ◽  
Marker Genes ◽  
Dermal Papilla Cells ◽  
Cell Groups

Introduction: Regarding the limited ability of the damaged cartilage cells to self-renew, which is due to their specific tissue structure, subtle damages can usually cause diseases such as osteoarthritis. In this work, using laser photobiomodulation and an interesting source of growth factors cocktail called the synovial fluid, we analyzed the chondrogenic marker genes in treated hair follicle dermal papilla cells as an accessible source of cells with relatively high differentiation potential. Methods: Dermal papilla cells were isolated from rat whisker hair follicle (Rattus norvegicus) and established cell cultures were treated with a laser (gallium aluminum arsenide diode Laser (λ=780 nm, 30 mW) at 5 J/cm2 ), the synovial fluid, and a combination of both. After 1, 4, 7, and 14 days, the morphological changes were evaluated and the expression levels of four chondrocyte marker genes (Col2a1, Sox-9, Col10a1, and Runx-2) were assessed by the quantitative real-time polymerase chain reaction. Results: It was monitored that treating cells with laser irradiation can accelerate the rate of proliferation of cells. The morphology of the cells treated with the synovial fluid altered considerably as in the fourth day they surprisingly looked like cultured articular chondrocytes. The gene expression analysis showed that all genes were up-regulated until the day 14 following the treatments although not equally in all the cell groups. Moreover, the cell groups treated with both irradiation and the synovial fluid had a significantly augmented expression in gene markers. Conclusion: Based on the gene expression levels and the morphological changes, we concluded that the synovial fluid can have the potential to make the dermal papilla cells to most likely mimic the chondrogenic and/or osteogenic differentiation, although this process seems to be augmented by the irradiation of the low-level laser.

scholarly journals Identification and Validation of a Potential Marker of Tissue Quality Using Gene Expression Analysis of Human Colorectal Tissue

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0133987 ◽  
Author(s):  
Nicole Lange ◽  
Florian T. Unger ◽  
Monika Schöppler ◽  
Katja Pursche ◽  
Hartmut Juhl ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Tissue Quality ◽  
Colorectal Tissue ◽  
Potential Marker
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