RNAi-based interactions: Persistent and acute viral infections in lepidopteran cell lines

2016 ◽  
Author(s):  
Dulce Santos
Keyword(s):  
Cell Lines ◽  
Viral Infections ◽  
Lepidopteran Cell Lines

Differential response of the Senegalese sole (Solea senegalensis) Mx promoter to viral infections in two salmonid cell lines

2014 ◽  
Vol 161 (3-4) ◽  
pp. 251-257 ◽  
Author(s):  
Daniel Alvarez-Torres ◽  
M. Carmen Alonso ◽  
Esther Garcia-Rosado ◽  
Bertrand Collet ◽  
Julia Béjar
Keyword(s):  
Cell Lines ◽  
Viral Infections ◽  
Differential Response ◽  
Solea Senegalensis ◽  
Senegalese Sole

Replication of an entomopoxvirus in two lepidopteran cell lines

1990 ◽  
Vol 56 (2) ◽  
pp. 222-232 ◽  
Author(s):  
Tosihiko Hukuhara ◽  
Jinhua Xu ◽  
Kazuhiko Yano
Keyword(s):  
Cell Lines ◽  
Lepidopteran Cell Lines

Isozyme characterization of 28 cell lines from five insect species

1985 ◽  
Vol 63 (10) ◽  
pp. 2270-2276 ◽  
Author(s):  
G. T. Harvey ◽  
S. S. Sohi
Keyword(s):  
Cell Lines ◽  
Cell Cultures ◽  
Choristoneura Fumiferana ◽  
Insect Species ◽  
Supporting Evidence ◽  
Starch Gel Electrophoresis ◽  
Species Identity ◽  
Starch Gel ◽  
Lepidopteran Cell Lines

Correct identity of cell lines is essential for their use in any investigation; isozyme patterns of cell cultures can give reliable identification. Starch gel electrophoresis was used to develop isozyme profiles of 8 hymenopteran and 20 lepidopteran cell lines and of the insect species from which they were developed. Species identity of 26 of the cell lines was confirmed. For nine of the cell lines these results support the identity established by serological and chromosomal analyses. For the remaining cell lines they provide the first confirmation of species identity. Isozyme profiles of several cell lines from the same species showed unique characteristics that will be useful in monitoring their identity. Two cell lines (IPRI-OL-7 and IPRI-OL-11) considered to be from Orgyia leucostigma appear to contain isozymes of Choristoneura fumiferana. Other supporting evidence and possible causes of this contamination are discussed. These results demonstrate the usefulness of isozyme profiles for the identification and monitoring of cell cultures.

scholarly journals Network Analysis and Transcriptome Profiling Identify Autophagic and Mitochondrial Dysfunctions in SARS-CoV-2 Infection

2020 ◽  
Author(s):  
Komudi Singh ◽  
Yun-Ching Chen ◽  
Jennifer T Judy ◽  
Fayaz Seifuddin ◽  
Ilker Tunc ◽  
...  
Keyword(s):  
Cell Lines ◽  
Influenza A ◽  
Viral Infections ◽  
Expression Profiles ◽  
Transcriptome Profiling ◽  
Interferon Response ◽  
Autophagic Flux ◽  
Human Samples ◽  
Transcriptional Changes ◽  
Infected Cells

AbstractAnalyzing host transcriptional changes in response to SARS-CoV-2 infection will help delineate biological processes underlying viral pathogenesis. Comparison of expression profiles of lung cell lines A549 (infected with either SARS-CoV-2 (with ACE2 expression)) or Influenza A virus (IAV)) and Calu3 (infected with SARS-CoV-2 or MERS-CoV) revealed upregulation of the antiviral interferon signaling in all three viral infections. However, perturbations in inflammatory, mitochondrial, and autophagy processes were specifically observed in SARS-CoV-2 infected cells. Validation of findings from cell line data revealed perturbations in autophagy and mitochondrial processes in the infected human nasopharyngeal samples. Specifically, downregulation of mTOR expression, mitochondrial ribosomal, mitochondrial complex I, and lysosome acidification genes were concurrently observed in both infected cell lines and human datasets. Furthermore, SARS-CoV-2 infection impedes autophagic flux by upregulating GSK3B in lung cell lines, or by downregulating autophagy genes, SNAP29 and lysosome acidification genes in human samples, contributing to increased viral replication. Therefore, drugs targeting lysosome acidification or autophagic flux could be tested as intervention strategies. Additionally, downregulation of MTFP1 (in cell lines) or SOCS6 (in human samples) results in hyperfused mitochondria and impede proper interferon response. Coexpression networks analysis identifies correlated clusters of genes annotated to inflammation and mitochondrial processes that are misregulated in SARS-CoV-2 infected cells. Finally, comparison of age stratified human gene expression data revealed impaired upregulation of chemokines, interferon stimulated and tripartite motif genes that are critical for antiviral signaling. Together, this analysis has revealed specific aspects of autophagic and mitochondrial function that are uniquely perturbed in SARS-CoV-2 infection.

scholarly journals Txp40, a Ubiquitous Insecticidal Toxin Protein from Xenorhabdus and Photorhabdus Bacteria

2006 ◽  
Vol 72 (2) ◽  
pp. 1653-1662 ◽  
Author(s):  
S. E. Brown ◽  
A. T. Cao ◽  
P. Dobson ◽  
E. R. Hines ◽  
R. J. Akhurst ◽  
...  
Keyword(s):  
Cell Lines ◽  
Galleria Mellonella ◽  
Fat Body ◽  
Direct Injection ◽  
Toxin Gene ◽  
Xenorhabdus Nematophila ◽  
Insecticidal Toxin ◽  
Histological Data ◽  
Toxin Protein ◽  
Lepidopteran Cell Lines

ABSTRACT Xenorhabdus and Photorhabdus are gram-negative bacteria that produce a range of proteins that are toxic to insects. We recently identified a novel 42-kDa protein from Xenorhabdus nematophila that was lethal to the larvae of insects such as Galleria mellonella and Helicoverpa armigera when it was injected at doses of 30 to 40 ng/g larvae. In the present work, the toxin gene txp40 was identified in another 59 strains of Xenorhabdus and Photorhabdus, indicating that it is both highly conserved and widespread among these bacteria. Recombinant toxin protein was shown to be active against a variety of insect species by direct injection into the larvae of the lepidopteran species G. mellonella, H. armigera, and Plodia interpunctella and the dipteran species Lucilia cuprina. The protein exhibited significant cytotoxicity against two dipteran cell lines and two lepidopteran cell lines but not against a mammalian cell line. Histological data from H. armigera larvae into which the toxin was injected suggested that the primary site of action of the toxin is the midgut, although some damage to the fat body was also observed.

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