The extent of RNAi in the arbovirus vector,Culicoides sonorensis

2016 ◽  
Author(s):  
Mary Katherine Mills
2005 ◽  
Vol 14 (2) ◽  
pp. 121-136 ◽  
Author(s):  
C. L. Campbell ◽  
K. A. Vandyke ◽  
G. J. Letchworth ◽  
B. S. Drolet ◽  
T. Hanekamp ◽  
...  

2014 ◽  
Vol 24 (1) ◽  
pp. 105-114 ◽  
Author(s):  
M. K. Mills ◽  
D. Nayduch ◽  
K. Michel

Author(s):  
Velmurugan Balaraman ◽  
Barbara S Drolet ◽  
Natasha N Gaudreault ◽  
William C Wilson ◽  
Jeana Owens ◽  
...  

Abstract SARS-CoV-2 is a recently emerged, highly contagious virus and the cause of the current COVID-19 pandemic. It is a zoonotic virus, although its animal origin is not clear yet. Person-to-person transmission occurs by inhalation of infected droplets and aerosols, or by direct contact with contaminated fomites. Arthropods transmit numerous viral, parasitic, and bacterial diseases; however, the potential role of arthropods in SARS-CoV-2 transmission is not fully understood. Thus far, a few studies have demonstrated that SARS-CoV-2 replication is not supported in cells from certain insect species nor in certain species of mosquitoes after intrathoracic inoculation. In this study, we expanded the work of SARS-CoV-2 susceptibility to biting insects after ingesting a SARS-CoV-2-infected bloodmeal. Species tested included Culicoides sonorensis (Wirth & Jones) (Diptera: Ceratopogonidae) biting midges, as well as Culex tarsalis (Coquillett) and Culex quinquefasciatus (Say) mosquitoes (Diptera: Culicidae), all known biological vectors for numerous RNA viruses. Arthropods were allowed to feed on SARS-CoV-2-spiked blood and at a time point postinfection analyzed for the presence of viral RNA and infectious virus. Additionally, cell lines derived from C. sonorensis (W8a), Aedes aegypti (Linnaeus) (Diptera: Culicidae) (C6/36), Cx. quinquefasciatus (HSU), and Cx. tarsalis (CxTrR2) were tested for SARS-CoV-2 susceptibility. Our results indicate that none of the biting insects, nor the insect cell lines evaluated support SARS-CoV-2 replication, suggesting that these species are unable to be biological vectors of SARS-CoV-2.


PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0130294 ◽  
Author(s):  
Anna Zuliani ◽  
Alessandro Massolo ◽  
Timothy Lysyk ◽  
Gregory Johnson ◽  
Shawn Marshall ◽  
...  

2015 ◽  
Vol 9 (9) ◽  
pp. e0004060 ◽  
Author(s):  
Veronika Seblova ◽  
Jovana Sadlova ◽  
Barbora Vojtkova ◽  
Jan Votypka ◽  
Simon Carpenter ◽  
...  

2012 ◽  
Vol 112 (3) ◽  
pp. 991-999 ◽  
Author(s):  
Barbara Conti ◽  
Michele Leonardi ◽  
Luisa Pistelli ◽  
Raffaele Profeti ◽  
Ines Ouerghemmi ◽  
...  

2008 ◽  
Vol 45 (5) ◽  
pp. 921-926 ◽  
Author(s):  
Kristine E. Bennett ◽  
Jessica E. Hopper ◽  
Melissa A. Stuart ◽  
Mark West ◽  
Barbara S. Drolet

Sign in / Sign up

Export Citation Format

Share Document