Genomics of bluetongue virus vector competence inCulicoides sonorensis(Diptera: Ceratopogonidae)

2016 ◽  
Author(s):  
Ramiro Morales-Hojas
Keyword(s):  
Bluetongue Virus ◽  
Vector Competence ◽  
Virus Vector

Expansion of Culicoides imicola, the main bluetongue virus vector in Europe, into Catalonia, Spain

2005 ◽  
Vol 156 (13) ◽  
pp. 415-417 ◽  
Author(s):  
V. Sarto i Monteys ◽  
D. Ventura ◽  
N. Pages ◽  
C. Aranda ◽  
R. Escosa
Keyword(s):  
Bluetongue Virus ◽  
Virus Vector ◽  
Culicoides Imicola

scholarly journals An assessment ofCulicoidessurveillance techniques in northern Europe: have we underestimated a potential bluetongue virus vector?

2008 ◽  
pp. ???-??? ◽  
Author(s):  
Simon Carpenter ◽  
Camille Szmaragd ◽  
James Barber ◽  
Karien Labuschagne ◽  
Simon Gubbins ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Virus Vector ◽  
Northern Europe

scholarly journals Vector competence is strongly affected by a small deletion or point mutations in bluetongue virus

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
René G. P. van Gennip ◽  
Barbara S. Drolet ◽  
Paula Rozo Lopez ◽  
Ashley J. C. Roost ◽  
Jan Boonstra ◽  
...  
Keyword(s):  
Virus Replication ◽  
Bluetongue Virus ◽  
Vector Competence ◽  
Outer Shell ◽  
Virus Propagation ◽  
Virus Growth ◽  
Shell Protein ◽  
Vector Borne

Abstract Background Transmission of vector-borne virus by insects is a complex mechanism consisting of many different processes; viremia in the host, uptake, infection and dissemination in the vector, and delivery of virus during blood-feeding leading to infection of the susceptible host. Bluetongue virus (BTV) is the prototype vector-borne orbivirus (family Reoviridae). BTV serotypes 1–24 (typical BTVs) are transmitted by competent biting Culicoides midges and replicate in mammalian (BSR) and midge (KC) cells. Previously, we showed that genome segment 10 (S10) encoding NS3/NS3a protein is required for virus propagation in midges. BTV serotypes 25–27 (atypical BTVs) do not replicate in KC cells. Several distinct BTV26 genome segments cause this so-called ‘differential virus replication’ in vitro. Methods Virus strains were generated using reverse genetics and their growth was examined in vitro. The midge feeding model has been developed to study infection, replication and disseminations of virus in vivo. A laboratory colony of C. sonorensis, a known competent BTV vector, was fed or injected with BTV variants and propagation in the midge was examined using PCR testing. Crossing of the midgut infection barrier was examined by separate testing of midge heads and bodies. Results A 100 nl blood meal containing ±105.3 TCID50/ml of BTV11 which corresponds to ±20 TCID50 infected 50% of fully engorged midges, and is named one Midge Alimentary Infective Dose (MAID50). BTV11 with a small in-frame deletion in S10 infected blood-fed midge midguts but virus release from the midgut into the haemolymph was blocked. BTV11 with S1[VP1] of BTV26 could be adapted to virus growth in KC cells, and contained mutations subdivided into ‘corrections’ of the chimeric genome constellation and mutations associated with adaptation to KC cells. In particular one amino acid mutation in outer shell protein VP2 overcomes differential virus replication in vitro and in vivo. Conclusion Small changes in NS3/NS3a or in the outer shell protein VP2 strongly affect virus propagation in midges and thus vector competence. Therefore, spread of disease by competent Culicoides midges can strongly differ for very closely related viruses.

scholarly journals Vector Competence of Italian Populations of Culicoides for Some Bluetongue Virus Strains Responsible for Recent Northern African and European Outbreaks

Viruses ◽  
2019 ◽  
Vol 11 (10) ◽  
pp. 941 ◽  
Author(s):  
Valentina Federici ◽  
Maria Goffredo ◽  
Giuseppe Mancini ◽  
Michela Quaglia ◽  
Adriana Santilli ◽  
...  
Keyword(s):  
Bluetongue Virus ◽  
Vector Competence ◽  
Oral Infection ◽  
Balearic Islands ◽  
Rt Pcr ◽  
Vector Species ◽  
Vaccination Programs ◽  
Culicoides Obsoletus ◽  
Culicoides Imicola ◽  
Virus Strains

The distribution of Bluetongue virus (BTV) in Europe can be represented by two distinct and interconnected epidemiological systems (episystems), each characterized by different ecological characteristics and vector species. This study investigated the vector competence of Italian populations of Culicoides imicola and Culicoides obsoletus/scoticus to some representative BTV strains after artificial oral infection. The BTV strains were selected according to their ability to spread to one or both episystems and included BTV-4 ITA, responsible of the recent Italian and French BTV-4 outbreaks; the BTV-2 strain which caused the first BTV incursion in Italy, Corsica, and Balearic Islands; BTV-4 MOR, responsible for the epidemic in Morocco; and BTV-8, the strain which spread through Europe between 2006 and 2008. Blood-soaked cotton pledgets and Hemotek membrane feeder using Parafilm® membrane were used to artificially feed midges. For each population/strain, recovery rates (positive/tested heads) were evaluated using serogroup- and serotype-specific RT-PCR. The trial demonstrated that, except for the Abruzzo population of C. obsoletus/C. scoticus, which was refractory to BTV-4 MOR infection, all the investigated Culicoides populations are susceptible to the selected BTV strains and that, if prompt vaccination programs and restriction measures had not been implemented, BTV-2 and BTV-4 MOR could have spread all over Europe.

scholarly journals Country-wide distribution of bluetongue virus with expanding host spectrum and evidence of vector competence in Hungary

2019 ◽  
Vol 63 (02) ◽  
pp. 229-234
Author(s):  
M. DOMÁN ◽  
S. MARTON ◽  
P. MALIK ◽  
K. BÁNYAI ◽  
Á. HORNYÁK
Keyword(s):  
Bluetongue Virus ◽  
Vector Competence ◽  
Wide Distribution ◽  
Host Spectrum

Understanding the mechanism of Chikungunya virus vector competence in three species of mosquitoes

2019 ◽  
Vol 33 (3) ◽  
pp. 375-387
Author(s):  
A. Ghosh ◽  
T. Mullapudi ◽  
S. Bomanna ◽  
B. K. Tyagi ◽  
V. Ravi ◽  
...  
Keyword(s):  
Chikungunya Virus ◽  
Vector Competence ◽  
Virus Vector

scholarly journals Testing of UK Populations of Culex pipiens L. for Schmallenberg Virus Vector Competence and Their Colonization

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0134453 ◽  
Author(s):  
Robyn Manley ◽  
Lara E. Harrup ◽  
Eva Veronesi ◽  
Francesca Stubbins ◽  
Jo Stoner ◽  
...  
Keyword(s):  
Culex Pipiens ◽  
Vector Competence ◽  
Virus Vector ◽  
Schmallenberg Virus

scholarly journals The genome of the biting midge Culicoides sonorensis and gene expression analyses of vector competence for bluetongue virus

BMC Genomics ◽  
2018 ◽  
Vol 19 (1) ◽  
Author(s):  
Ramiro Morales-Hojas ◽  
Malcolm Hinsley ◽  
Irina M. Armean ◽  
Rhiannon Silk ◽  
Lara E. Harrup ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bluetongue Virus ◽  
Vector Competence ◽  
Biting Midge ◽  
Culicoides Sonorensis ◽  
Gene Expression Analyses
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