Impact of Transient Heat Stress on Polar Lipid Metabolism in Seedlings of Wheat Near-Isogenic Lines Contrasting in Resistance to Hessian Fly (Cecidomyiidae) Infestation

2014 ◽  
Vol 107 (6) ◽  
pp. 2196-2203 ◽  
Author(s):  
Yaleaka Currie ◽  
Ming-Shun Chen ◽  
Radoslav Nickolov ◽  
Guihua Bai ◽  
Lieceng Zhu
Keyword(s):  
Heat Stress ◽  
Lipid Metabolism ◽  
Polar Lipid ◽  
Hessian Fly ◽  
Near Isogenic Lines ◽  
Isogenic Lines

Comparative analysis of genetic background in eight near-isogenic wheat lines with different H genes conferring resistance to Hessian fly

Genome ◽  
2011 ◽  
Vol 54 (1) ◽  
pp. 81-89 ◽  
Author(s):  
S. S. Xu ◽  
C. G. Chu ◽  
M. O. Harris ◽  
C. E. Williams
Keyword(s):  
Genetic Background ◽  
Genetic Similarity ◽  
Triticum Aestivum L ◽  
Hessian Fly ◽  
Pedigree Analysis ◽  
Recurrent Parent ◽  
Linkage Drag ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
H Gene

Near-isogenic lines (NILs) are useful for plant genetic and genomic studies. However, the strength of conclusions from such studies depends on the similarity of the NILs’ genetic backgrounds. In this study, we investigated the genetic similarity for a set of NILs developed in the 1990s to study gene-for-gene interactions between wheat ( Triticum aestivum L.) and the Hessian fly ( Mayetiola destructor (Say)), an important pest of wheat. Each of the eight NILs carries a single H resistance gene and was created by successive backcrossing for two to six generations to susceptible T. aestivum ‘Newton’. We generated 256 target region amplification polymorphism (TRAP) markers and used them to calculate genetic similarity, expressed by the Nei and Li (NL) coefficient. Six of the NILs (H3, H5, H6, H9, H11, and H13) had the highly uniform genetic background of Newton, with NL coefficients from 0.97 to 0.99. However, genotypes with H10 or H12 were less similar to Newton, with NL coefficients of 0.86 and 0.93, respectively. Cluster analysis based on NL coefficients and pedigree analysis showed that the genetic similarity between each of the NILs and Newton was affected by both the number of backcrosses and the genetic similarity between Newton and the H gene donors. We thus generated an equation to predict the number of required backcrosses, given varying similarity of donor and recurrent parent. We also investigated whether the genetic residues of the donor parents that remained in the NILs were related to linkage drag. By using a complete set of ‘Chinese Spring’ nullisomic-tetrasomic lines, one third of the TRAP markers that showed polymorphism between the NILs and Newton were assigned to a specific chromosome. All of the assigned markers were located on chromosomes other than the chromosome carrying the H gene, suggesting that the genetic residues detected in this study were not due to linkage drag. Results will aid in the development and use of near-isogenic lines for studies of the functional genomics of wheat.

Effect of heat stress on wheat proteins during kernel development in wheat near-isogenic lines differing at Glu-D1

2008 ◽  
Vol 48 (2) ◽  
pp. 513-516 ◽  
Author(s):  
Sibel Irmak ◽  
Hamid A. Naeem ◽  
George L. Lookhart ◽  
Finlay MacRitchie
Keyword(s):  
Heat Stress ◽  
Near Isogenic Lines ◽  
Wheat Proteins ◽  
Kernel Development ◽  
Isogenic Lines

scholarly journals Comparison of photosynthetic activity and heat tolerance between near isogenic lines of wheat with different photosynthetic rates

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0255896
Author(s):  
Chongyang Li ◽  
Mingyang Ma ◽  
Tianpeng Zhang ◽  
Pengwen Feng ◽  
Xiao Chen ◽  
...  
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Photosynthetic Rate ◽  
Calvin Cycle ◽  
Grain Filling ◽  
Near Isogenic Lines ◽  
Photosynthetic Rates ◽  
Isogenic Lines ◽  
Filling Stage ◽  
Grain Filling Stage

Wheat (Triticum aestivum L.) is one of the most important crops in the world, but the yield and quality of wheat are highly susceptible to heat stress, especially during the grain-filling stage. Therefore, it is crucial to select high-yield and high-temperature-resistant varieties for food cultivation. There is a positive correlation between the yield and photosynthetic rate of wheat during the entire grain-filling stage, but few studies have shown that lines with high photosynthetic rates can maintain higher thermotolerance at the same time. In this study, two pairs of wheat near isogenic lines (NILs) with different photosynthetic rates were used for all experiments. Our results indicated that under heat stress, lines with a high photosynthetic rate could maintain the activities of photosystem II (PSII) and key Calvin cycle enzymes in addition to their higher photosynthetic rates. The protein levels of D1 and HSP70 were significantly increased in the highly photosynthetic lines, which contributed to maintaining high photosynthetic rates and ensuring the stability of the Calvin cycle under heat stress. Furthermore, we found that lines with a high photosynthetic rate could maintain high antioxidant enzyme activity to scavenge reactive oxygen species (ROS) and reduce ROS accumulation better than lines with a low photosynthetic rate under high-temperature stress. These findings suggest that lines with high photosynthetic rates can maintain a higher photosynthetic rate despite heat stress and are more thermotolerant than lines with low photosynthetic rates.

scholarly journals Primary metabolite contents are correlated with seed protein and oil traits in near-isogenic lines of soybean

2019 ◽  
Vol 7 (5) ◽  
pp. 651-659 ◽  
Author(s):  
Jie Wang ◽  
Pengfei Zhou ◽  
Xiaolei Shi ◽  
Na Yang ◽  
Long Yan ◽  
...  
Keyword(s):  
Seed Protein ◽  
Near Isogenic Lines ◽  
Primary Metabolite ◽  
Isogenic Lines

scholarly journals The Impact of Climate Change on the Resistance of Rice Near-Isogenic Lines with Resistance Genes Against Brown Planthopper

Rice ◽  
2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yun-Hung Kuang ◽  
Yu-Fu Fang ◽  
Shau-Ching Lin ◽  
Shin-Fu Tsai ◽  
Zhi-Wei Yang ◽  
...  
Keyword(s):  
Climate Change ◽  
Resistance Genes ◽  
Environmental Changes ◽  
Gene Pyramiding ◽  
Brown Planthopper ◽  
Inhibitory Effect ◽  
Near Isogenic Lines ◽  
Impact Of Climate Change ◽  
Isogenic Lines ◽  
The Impact

Abstract Background The impact of climate change on insect resistance genes is elusive. Hence, we investigated the responses of rice near-isogenic lines (NILs) that carry resistance genes against brown planthopper (BPH) under different environmental conditions. Results We tested these NILs under three environmental settings (the atmospheric temperature with corresponding carbon dioxide at the ambient, year 2050 and year 2100) based on the Intergovernmental Panel on Climate Change prediction. Comparing between different environments, two of nine NILs that carried a single BPH-resistant gene maintained their resistance under the environmental changes, whereas two of three NILs showed gene pyramiding with two maintained BPH resistance genes despite the environmental changes. In addition, two NILs (NIL-BPH17 and NIL-BPH20) were examined in their antibiosis and antixenosis effects under these environmental changes. BPH showed different responses to these two NILs, where the inhibitory effect of NIL-BPH17 on the BPH growth and development was unaffected, while NIL-BPH20 may have lost its resistance during the environmental changes. Conclusion Our results indicate that BPH resistance genes could be affected by climate change. NIL-BPH17 has a strong inhibitory effect on BPH feeding on phloem and would be unaffected by environmental changes, while NIL-BPH20 would lose its ability during the environmental changes.

scholarly journals Drought Tolerant near Isogenic Lines (NILs) of Pusa 44 Developed through Marker Assisted Introgression of qDTY2.1 and qDTY3.1 Enhances Yield under Reproductive Stage Drought Stress

Agriculture ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 64
Author(s):  
Priyanka Dwivedi ◽  
Naleeni Ramawat ◽  
Gaurav Dhawan ◽  
Subbaiyan Gopala Krishnan ◽  
Kunnummal Kurungara Vinod ◽  
...  
Keyword(s):  
Drought Stress ◽  
Drought Tolerance ◽  
Grain Quality ◽  
Reproductive Stage ◽  
Recurrent Parent ◽  
Near Isogenic Lines ◽  
Improvement Project ◽  
Isogenic Lines ◽  
Drought Tolerant ◽  
Rice Improvement

Reproductive stage drought stress (RSDS) is detrimental for rice, which affects its productivity as well as grain quality. In the present study, we introgressed two major quantitative trait loci (QTLs), namely, qDTY2.1 and qDTY3.1, governing RSDS tolerance in a popular high yielding non-aromatic rice cultivar, Pusa 44, through marker-assisted backcross breeding (MABB). Pusa 44 is highly sensitive to RSDS, which restricts its cultivation across drought-prone environments. Foreground selection was carried out using markers, RM520 for qDTY3.1 and RM 521 for qDTY2.1. Background selection was achieved with 97 polymorphic SSR markers in tandem with phenotypic selection to achieve faster recurrent parent genome (RPG) recovery. Three successive backcrosses followed by three selfings aided RPG recoveries of 98.6% to 99.4% among 31 near isogenic lines (NILs). Fourteen NILs were found to be significantly superior in yield and grain quality under RSDS with higher drought tolerance efficiency (DTE) than Pusa 44. Among these, the evaluation of two promising NILs in the multilocational trial during Kharif 2019 showed that they were significantly superior to Pusa 44 under reproductive stage drought stress, while performing on par with Pusa 44 under normal irrigated conditions. These di-QTL pyramided drought-tolerant NILs are in the final stages of testing the All India Coordinated Rice Improvement Project varietal trials for cultivar release. Alternately, the elite drought-tolerant Pusa 44 NILs will serve as an invaluable source of drought tolerance in rice improvement.

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