Development and Validation of Real-Time PCR Tests for the Identification of Four Spodoptera Species: Spodoptera eridania, Spodoptera frugiperda, Spodoptera littoralis, and Spodoptera litura (Lepidoptera: Noctuidae)

2014 ◽  
Vol 107 (4) ◽  
pp. 1643-1654 ◽  
Author(s):  
B.T.L.H. Van De Vossenberg ◽  
M. J. Van Der Straten
Keyword(s):  
Real Time ◽  
Spodoptera Frugiperda ◽  
Real Time Pcr ◽  
Spodoptera Littoralis ◽  
Spodoptera Litura ◽  
Spodoptera Eridania ◽  
Development And Validation ◽  
Lepidoptera Noctuidae

scholarly journals Development and validation of a triplex real-time PCR for rapid detection and specific identification of M. avium sub sp. paratuberculosis in faecal samples

2009 ◽  
Vol 136 (1-2) ◽  
pp. 166-172 ◽  
Author(s):  
Léonid M. Irenge ◽  
Karl Walravens ◽  
Marc Govaerts ◽  
Jacques Godfroid ◽  
Valérie Rosseels ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Specific Identification ◽  
Faecal Samples ◽  
Development And Validation

scholarly journals Development and validation of qualitative SYBR®Green Real-Time PCR for detection and discrimination of Listeria spp. and Listeria monocytogenes

2012 ◽  
Vol 97 (9) ◽  
pp. 4021-4037 ◽  
Author(s):  
Elodie Barbau-Piednoir ◽  
Nadine Botteldoorn ◽  
Marc Yde ◽  
Jacques Mahillon ◽  
Nancy H. Roosens
Keyword(s):  
Listeria Monocytogenes ◽  
Real Time ◽  
Real Time Pcr ◽  
Development And Validation

Development and validation of TaqMan real-time PCR assays for quantification of chicken adulteration in hamburgers

2021 ◽  
pp. 104302
Author(s):  
Zahra Sarlak ◽  
Saeedeh Shojaee-Aliabadi ◽  
Nayebali Rezvani ◽  
Hedayat Hosseini ◽  
Milad Rouhi ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assays ◽  
Development And Validation

scholarly journals Sesame, Pistachio, and Macadamia Nut: Development and Validation of New Allergenic Systems for Fast Real-Time PCR Application

Foods ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1085
Author(s):  
Martina Torricelli ◽  
Elisa Pierboni ◽  
Cristina Rondini ◽  
Serena Altissimi ◽  
Naceur Haouet
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Food Processing ◽  
Annealing Temperature ◽  
Limit Of Detection ◽  
Food Samples ◽  
Support Tool ◽  
Macadamia Nut ◽  
Real Market ◽  
Development And Validation

Food allergy is a worldwide health problem that concerns infants to adults. The main health risk for sensitised individuals is due to the presence of traces of allergens as the result of an accidental contamination during food processing. The labelling of allergens such as sesame, pistachio, and macadamia nut on food products is mandatory according to Regulation (EU) N. 1169/2011; therefore, the development of suitable and specific analytical methodologies is advisable. The aim of this study was to perform a multi-allergen real-time PCR system that works well in fast mode at the same annealing temperature and with the same thermal profile. The real-time PCR was developed designing new, specific, and efficient primer and probe systems for the 2S albumingene for sesame and pistachio and for the vicilin precursorgene for macadamia nut. These systems were subjected to a robust intra-laboratory qualitative validation process prior to their application, by DNA extraction and fast real-time PCR, on some real market samples to reproduce a potential allergen contamination along the food chain. The developed system results were specific and robust, with a sensible limit of detection (0.005% for sesame; 0.004% for pistachio; 0.006% for macadamia nut). The performance and the reliability of the target systems were confirmed on commercial food samples. This molecular approach could be used as a screening or as a support tool, in association with the other widespread monitoring techniques (such as ELISA).

scholarly journals Correction for Hilbert et al., Development and Validation of a Highly Accurate Quantitative Real-Time PCR Assay for Diagnosis of Bacterial Vaginosis

2016 ◽  
Vol 54 (7) ◽  
pp. 1930-1930 ◽  
Author(s):  
David W. Hilbert ◽  
William L. Smith ◽  
Sean G. Chadwick ◽  
Geoffrey Toner ◽  
Eli Mordechai ◽  
...  
Keyword(s):  
Bacterial Vaginosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Quantitative Real Time Pcr ◽  
Pcr Assay ◽  
Development And Validation
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