Culture-Independent Analysis of Bacterial Communities in the Gut of Rice Water Weevil (Coleoptera: Curculionidae)

2014 ◽  
Vol 107 (3) ◽  
pp. 592-600 ◽  
Author(s):  
Fang Lu ◽  
Xiaoying Kang ◽  
Gus Lorenz ◽  
Luis Espino ◽  
Mingxing Jiang ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Rice Water Weevil ◽  
Independent Analysis ◽  
Culture Independent ◽  
Rice Water

scholarly journals Culture‐independent analysis of hydrocarbonoclastic bacterial communities in environmental samples during oil‐bioremediation

2018 ◽  
Vol 8 (2) ◽  
pp. e00630 ◽  
Author(s):  
Narjes Dashti ◽  
Nedaa Ali ◽  
Samar Salamah ◽  
Majida Khanafer ◽  
Ghada Al‐Shamy ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Environmental Samples ◽  
Independent Analysis ◽  
Culture Independent

scholarly journals Comparison of specific endophytic bacterial communities in different developmental stages of Passiflora incarnata using culture‐dependent and culture‐independent analysis

2019 ◽  
Vol 8 (10) ◽  
Author(s):  
Marcela C. Goulart ◽  
Luis G. Cueva‐Yesquén ◽  
Kelly J. Hidalgo Martinez ◽  
Derlene Attili‐Angelis ◽  
Fabiana Fantinatti‐Garboggini
Keyword(s):  
Bacterial Communities ◽  
Developmental Stages ◽  
Independent Analysis ◽  
Culture Independent ◽  
Passiflora Incarnata ◽  
Culture Dependent

scholarly journals Bias problems in culture-independent analysis of environmental bacterial communities: a representative study on hydrocarbonoclastic bacteria

SpringerPlus ◽  
2013 ◽  
Vol 2 (1) ◽  
pp. 369 ◽  
Author(s):  
Husain Al-Awadhi ◽  
Narjis Dashti ◽  
Majida Khanafer ◽  
Dina Al-Mailem ◽  
Nidaa Ali ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Hydrocarbonoclastic Bacteria ◽  
Representative Study ◽  
Independent Analysis ◽  
Culture Independent

scholarly journals Culture-independent analysis of endophytic bacterial communities associated with Brazilian sugarcane

2013 ◽  
Vol 12 (4) ◽  
pp. 4549-4558 ◽  
Author(s):  
G.S. Magnani ◽  
L.M. Cruz ◽  
H. Weber ◽  
J.C. Bespalhok ◽  
E. Daros ◽  
...  
Keyword(s):  
Bacterial Communities ◽  
Independent Analysis ◽  
Culture Independent

scholarly journals Chromosome Unipolar Division and Low Expression of Tws May Cause Parthenogenesis of Rice Water Weevil (Lissorhoptrus oryzophilus Kuschel)

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 278
Author(s):  
Pengcheng Wang ◽  
Fangyuan Yang ◽  
Zhuo Ma ◽  
Runzhi Zhang
Keyword(s):  
Drosophila Melanogaster ◽  
Quantitative Pcr ◽  
Ovarian Tissue ◽  
Expression Patterns ◽  
Meiotic Spindle ◽  
Lissorhoptrus Oryzophilus ◽  
Rice Water Weevil ◽  
Rice Water ◽  
Beijing China ◽  
Low Expression

Rice water weevil (RWW) is divided into two types of population, triploid parthenogenesis and diploid bisexual reproduction. In this study, we explored the meiosis of triploid parthenogenesis RWW (Shangzhuang Town, Haidian District, Beijing, China) by marking the chromosomes and microtubules of parthenogenetic RWW oocytes via immunostaining. The immunostaining results show that there is a canonical meiotic spindle formed in the triploid parthenogenetic RWW oocytes, but chromosomes segregate at only one pole, which means that there is a chromosomal unipolar division during the oogenesis of the parthenogenetic RWW. Furthermore, we cloned the conserved sequences of parthenogenetic RWW REC8 and Tws, and designed primers based on the parthenogenetic RWW sequence to detect expression patterns by quantitative PCR (Q-PCR). Q-PCR results indicate that the expression of REC8 and Tws in ovarian tissue of bisexual Drosophila melanogaster is 0.98 and 10,000.00 times parthenogenetic RWW, respectively (p < 0.01). The results show that Tws had low expression in parthenogenetic RWW ovarian tissue, and REC8 was expressed normally. Our study suggests that the chromosomal unipolar division and deletion of Tws may cause parthenogenesis in RWW.

Integration of culture-dependent and independent methods provides a more coherent picture of the pig gut microbiome

2020 ◽  
Vol 96 (3) ◽  
Author(s):  
Gavin J Fenske ◽  
Sudeep Ghimire ◽  
Linto Antony ◽  
Jane Christopher-Hennings ◽  
Joy Scaria
Keyword(s):  
Bacterial Communities ◽  
Bacterial Species ◽  
Shotgun Metagenomics ◽  
Culture Techniques ◽  
Microbiome Composition ◽  
Culture Independent ◽  
Health And Disease ◽  
The Media ◽  
And Function ◽  
Culture Dependent

ABSTRACT Bacterial communities resident in the hindgut of pigs, have profound impacts on health and disease. Investigations into the pig microbiome have utilized either culture-dependent, or far more commonly, culture-independent techniques using next generation sequencing. We contend that a combination of both approaches generates a more coherent view of microbiome composition. In this study, we surveyed the microbiome of Tamworth breed and feral pigs through the integration high throughput culturing and shotgun metagenomics. A single culture medium was used for culturing. Selective screens were added to the media to increase culture diversity. In total, 46 distinct bacterial species were isolated from the Tamworth and feral samples. Selective screens successfully shifted the diversity of bacteria on agar plates. Tamworth pigs are highly dominated by Bacteroidetes primarily composed of the genus Prevotella whereas feral samples were more diverse with almost equal proportions of Firmicutes and Bacteroidetes. The combination of metagenomics and culture techniques facilitated a greater retrieval of annotated genes than either method alone. The single medium based pig microbiota library we report is a resource to better understand pig gut microbial ecology and function. It allows for assemblage of defined bacterial communities for studies in bioreactors or germfree animal models.

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