Establishment and Gene Expression Characteristics of the Silk Gland Cell Line BmSG-SWU1 of Bombyx mori (Lepidoptera: Bombycidae)

2014 ◽  
Vol 107 (5) ◽  
pp. 1018-1026
Author(s):  
Zhen-Yue Feng ◽  
Chun Pan ◽  
Min Liu ◽  
Zhi-Qiang Tian ◽  
Xue-Mei Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Cell Line ◽  
Gland Cell ◽  
Silk Gland ◽  
Expression Characteristics

Changes in H+-translocating vacuolar-type ATPase in the anterior silk gland cell of Bombyx mori during metamorphosis.

1998 ◽  
Vol 201 (4) ◽  
pp. 479-486
Author(s):  
M Azuma ◽  
Y Ohta
Keyword(s):  
Bombyx Mori ◽  
Gland Cell ◽  
Specific Inhibitor ◽  
Instar Larva ◽  
Silk Gland ◽  
Ultrastructural Changes ◽  
Immunocytochemical Staining ◽  
Apical Plasma Membrane ◽  
Apical Surface ◽  
Silkworm Larvae

A proton-translocating vacuolar-type ATPase (V-ATPase) was identified and characterized in the anterior silk gland of Bombyx mori. By incubating the intact tissue with the fluorescent dye Acridine Orange, the acidified compartment was detected at the apical pole of the epithelial cells. This was observed throughout the feeding period of the fifth-instar larva until the onset of spinning. Acidification was prevented completely and reversibly by 0.8 micromol l-1 bafilomycin A1, a specific inhibitor of V-ATPase. The presence of V-ATPase in a microsomal fraction was verified by immunoblots using an antiserum to the V-ATPase holoenzyme from Manduca sexta midgut. The antiserum localized the V-ATPase to the apical plasma membrane of the anterior silk gland cells, suggesting that the enzyme is functionally active in pumping protons out of the cell towards the glandular lumen of feeding silkworm larvae. In spinning larvae, the acidification produced by the V-ATPase appears to cease, because acidic compartments were seen rarely and only in the periphery of basal cytoplasm, and because immunocytochemical staining for the V-ATPase was greatly reduced at the apical surface. The metamorphic changes in relation to the occurrence of V-ATPase corresponded well with the ultrastructural changes in the anterior silk gland cell of Bombyx mori larvae.

Effect of pyriproxyfen exposure on cocooning and gene expression in the silk gland of Bombyx mori （Linnaeus, 1758）

2020 ◽  
Vol 202 ◽  
pp. 110914
Author(s):  
Guodong Zhao ◽  
Xiao Zhang ◽  
Chentao Wang ◽  
Haitao Zhang ◽  
Huimin Guo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Silk Gland

scholarly journals Hox transcription factor Antp regulates sericin-1 gene expression in the terminal differentiated silk gland of Bombyx mori

2014 ◽  
Vol 386 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Mai Kimoto ◽  
Takuya Tsubota ◽  
Keiro Uchino ◽  
Hideki Sezutsu ◽  
Shigeharu Takiya
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Bombyx Mori ◽  
Silk Gland

scholarly journals Targeted Gene Expression Using the GAL4/UAS System in the Silkworm Bombyx mori

Genetics ◽  
2003 ◽  
Vol 165 (3) ◽  
pp. 1329-1340
Author(s):  
Morikazu Imamura ◽  
Junichi Nakai ◽  
Satoshi Inoue ◽  
Guo Xing Quan ◽  
Toshio Kanda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Gene Function ◽  
Germ Line ◽  
Silk Gland ◽  
Specific Expression ◽  
Efficient System ◽  
Gfp Fluorescence ◽  
Targeted Gene Expression ◽  
Silkworm Bombyx Mori

Abstract The silkworm Bombyx mori is one of the most well-studied insects in terms of both genetics and physiology and is recognized as the model lepidopteran insect. To develop an efficient system for analyzing gene function in the silkworm, we investigated the feasibility of using the GAL4/UAS system in conjunction with piggyBac vector-mediated germ-line transformation for targeted gene expression. To drive the GAL4 gene, we used two endogenous promoters that originated from the B. mori actin A3 (BmA3) and fibroin light-chain (FiL) genes and the artificial promoter 3xP3. GFP was used as the reporter. In initial tests of the function of the GAL4/UAS system, we generated transgenic animals that carried the UAS-GFP construct plus either BmA3-GAL4 or 3xP3-GAL4. GFP fluorescence was observed in the tissues of GFP-positive animals, in which both promoters drove GAL4 gene expression. Animals that possessed only the GAL4 gene or UAS-GFP construct did not show GFP fluorescence. In addition, as a further test of the ability of the GAL4/UAS system to drive tissue-specific expression we constructed FiL-GAL4 lines with 3xP3-CFP as the transformation marker. FiL-GAL4 × UAS-GFP crosses showed GFP expression in the posterior silk gland, in which the endogenous FiL gene is normally expressed. These results show that the GAL4/UAS system is applicable to B. mori and emphasize the potential of this system for controlled analyses of B. mori gene function.

Regulation of ACTH-R gene expression by CREB, CREMt and ICER in the adrenocortical cell line Y1

2005 ◽  
Vol 113 (S 1) ◽  
Author(s):  
O Zwermann ◽  
A Braun ◽  
E Lalli ◽  
F Beuschlein ◽  
M Reincke
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
R Gene ◽  
Adrenocortical Cell
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