Nomenclature Abstract for Chlorobaculum tepidum (Wahlund et al. 1996) Imhoff 2003.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Chlorobaculum Tepidum

scholarly journals Differential RNA Sequencing Implicates Sulfide as the Master Regulator of S 0 Metabolism in Chlorobaculum tepidum and Other Green Sulfur Bacteria

2017 ◽  
Vol 84 (3) ◽  
Author(s):  
Jacob M. Hilzinger ◽  
Vidhyavathi Raman ◽  
Kevin E. Shuman ◽  
Brian J. Eddie ◽  
Thomas E. Hanson
Keyword(s):  
Gene Expression ◽  
Elemental Sulfur ◽  
Green Sulfur Bacteria ◽  
Electron Donors ◽  
Reduced Sulfur Compounds ◽  
Promoter Elements ◽  
Content Type ◽  
Sulfur Bacteria ◽  
Chlorobaculum Tepidum ◽  
Green Sulfur

ABSTRACT The green sulfur bacteria ( Chlorobiaceae ) are anaerobes that use electrons from reduced sulfur compounds (sulfide, S 0 , and thiosulfate) as electron donors for photoautotrophic growth. Chlorobaculum tepidum , the model system for the Chlorobiaceae , both produces and consumes extracellular S 0 globules depending on the availability of sulfide in the environment. These physiological changes imply significant changes in gene regulation, which has been observed when sulfide is added to Cba. tepidum growing on thiosulfate. However, the underlying mechanisms driving these gene expression changes, i.e., the specific regulators and promoter elements involved, have not yet been defined. Here, differential RNA sequencing (dRNA-seq) was used to globally identify transcript start sites (TSS) that were present during growth on sulfide, biogenic S 0 , and thiosulfate as sole electron donors. TSS positions were used in combination with RNA-seq data from cultures growing on these same electron donors to identify both basal promoter elements and motifs associated with electron donor-dependent transcriptional regulation. These motifs were conserved across homologous Chlorobiaceae promoters. Two lines of evidence suggest that sulfide-mediated repression is the dominant regulatory mode in Cba. tepidum . First, motifs associated with genes regulated by sulfide overlap key basal promoter elements. Second, deletion of the Cba. tepidum 1277 ( CT1277 ) gene, encoding a putative regulatory protein, leads to constitutive overexpression of the sulfide:quinone oxidoreductase CT1087 in the absence of sulfide. The results suggest that sulfide is the master regulator of sulfur metabolism in Cba. tepidum and the Chlorobiaceae . Finally, the identification of basal promoter elements with differing strengths will further the development of synthetic biology in Cba. tepidum and perhaps other Chlorobiaceae . IMPORTANCE Elemental sulfur is a key intermediate in biogeochemical sulfur cycling. The photoautotrophic green sulfur bacterium Chlorobaculum tepidum either produces or consumes elemental sulfur depending on the availability of sulfide in the environment. Our results reveal transcriptional dynamics of Chlorobaculum tepidum on elemental sulfur and increase our understanding of the mechanisms of transcriptional regulation governing growth on different reduced sulfur compounds. This report identifies genes and sequence motifs that likely play significant roles in the production and consumption of elemental sulfur. Beyond this focused impact, this report paves the way for the development of synthetic biology in Chlorobaculum tepidum and other Chlorobiaceae by providing a comprehensive identification of promoter elements for control of gene expression, a key element of strain engineering.

scholarly journals Envelope Proteins of the CsmB/CsmF and CsmC/CsmD Motif Families Influence the Size, Shape, and Composition of Chlorosomes in Chlorobaculum tepidum

2009 ◽  
Vol 191 (22) ◽  
pp. 7109-7120 ◽  
Author(s):  
Hui Li ◽  
Donald A. Bryant
Keyword(s):  
Size Composition ◽  
Envelope Proteins ◽  
Structural Motif ◽  
Supramolecular Organization ◽  
Wild Type ◽  
Absorbance Maximum ◽  
Chlorobaculum Tepidum ◽  
Mutant Strains ◽  
Redundant Functions ◽  
Mutational Study

ABSTRACT The chlorosome envelope of Chlorobaculum tepidum contains 10 proteins that belong to four structural motif families. A previous mutational study (N.-U. Frigaard, H. Li, K. J. Milks, and D. A. Bryant, J. Bacteriol. 186:646-653, 2004) suggested that some of these proteins might have redundant functions. Six multilocus mutants were constructed to test the effects of eliminating the proteins of the CsmC/CsmD and CsmB/CsmF motif families, and the resulting strains were characterized physiologically and biochemically. Mutants lacking all proteins of either motif family still assembled functional chlorosomes, and as measured by growth rates of the mutant strains, light harvesting was affected only at the lowest light intensities tested (9 and 32 μmol photons m−2 s−1). The size, composition, and biogenesis of the mutant chlorosomes differed from those of wild-type chlorosomes. Mutants lacking proteins of the CsmC/CsmD motif family produced smaller chlorosomes than did the wild type, and the Qy absorbance maximum for the bacteriochlorophyll c aggregates in these chlorosomes was strongly blueshifted. Conversely, the chlorosomes of mutants lacking proteins of the CsmB/CsmF motif family were larger than wild-type chlorosomes, and the Qy absorption for their bacteriochlorophyll c aggregates was redshifted. When CsmH was eliminated in addition to other proteins of either motif family, chlorosomes had smaller diameters. These data show that the chlorosome envelope proteins of the CsmB/CsmF and CsmC/CsmD families play important roles in determining chlorosome size as well as the assembly and supramolecular organization of the bacteriochlorophyll c aggregates within the chlorosome.

Proteome profiling of the green sulfur bacterium Chlorobaculum tepidum by N-terminal proteomics

PROTEOMICS ◽  
2011 ◽  
Vol 12 (1) ◽  
pp. 63-67 ◽  
Author(s):  
Kalliopi Kouyianou ◽  
Pieter-Jan De Bock ◽  
Niklaas Colaert ◽  
Antigoni Nikolaki ◽  
Aikaterini Aktoudianaki ◽  
...  
Keyword(s):  
Green Sulfur Bacterium ◽  
Proteome Profiling ◽  
Chlorobaculum Tepidum ◽  
Green Sulfur

scholarly journals In situ high-resolution structure of the baseplate antenna complex in Chlorobaculum tepidum

2016 ◽  
Vol 7 (1) ◽  
Author(s):  
Jakob Toudahl Nielsen ◽  
Natalia V. Kulminskaya ◽  
Morten Bjerring ◽  
Juha M. Linnanto ◽  
Margus Rätsep ◽  
...  
Keyword(s):  
High Resolution ◽  
Antenna Complex ◽  
Chlorobaculum Tepidum ◽  
High Resolution Structure ◽  
Resolution Structure

scholarly journals Insights Into the Mineralogy and Surface Chemistry of Extracellular Biogenic S0 Globules Produced by Chlorobaculum tepidum

2019 ◽  
Vol 10 ◽  
Author(s):  
Cassandra L. Marnocha ◽  
Chandran R. Sabanayagam ◽  
Shannon Modla ◽  
Deborah H. Powell ◽  
Pauline A. Henri ◽  
...  
Keyword(s):  
Surface Chemistry ◽  
Chlorobaculum Tepidum

Chemical oxidation of the FMO antenna protein from Chlorobaculum tepidum

2013 ◽  
Vol 116 (1) ◽  
pp. 11-19 ◽  
Author(s):  
David Bina ◽  
Robert E. Blankenship
Keyword(s):  
Chemical Oxidation ◽  
Chlorobaculum Tepidum

scholarly journals Insights into the Excitonic States of Individual Chlorosomes from Chlorobaculum tepidum

2014 ◽  
Vol 106 (9) ◽  
pp. 1921-1927 ◽  
Author(s):  
Marc Jendrny ◽  
Thijs J. Aartsma ◽  
Jürgen Köhler
Keyword(s):  
Chlorobaculum Tepidum ◽  
Excitonic States
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