Nomenclature Abstract for Chlorogloeopsis fritschii McNeill et al. 2006.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Chlorogloeopsis Fritschii

scholarly journals Far-Red Light Acclimation for Improved Mass Cultivation of Cyanobacteria

Metabolites ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 170 ◽  
Author(s):  
Alla Silkina ◽  
Bethan Kultschar ◽  
Carole A. Llewellyn
Keyword(s):  
Biomass Production ◽  
White Light ◽  
Light Emitting Diode ◽  
Red Light ◽  
Industrial Biotechnology ◽  
Light Conditions ◽  
Light Emitting ◽  
Mass Cultivation ◽  
Chlorogloeopsis Fritschii ◽  
Bioactive Product

Improving mass cultivation of cyanobacteria is a goal for industrial biotechnology. In this study, the mass cultivation of the thermophilic cyanobacterium Chlorogloeopsis fritschii was assessed for biomass production under light-emitting diode white light (LEDWL), far-red light (FRL), and combined white light and far-red light (WLFRL) adaptation. The induction of chl f was confirmed at 24 h after the transfer of culture from LEDWL to FRL. Using combined light (WLFRL), chl f, a, and d, maintained the same level of concentration in comparison to FRL conditions. However, phycocyanin and xanthophylls (echinone, caloxanthin, myxoxanthin, nostoxanthin) concentration increased 2.7–4.7 times compared to LEDWL conditions. The productivity of culture was double under WLFRL compared with LEDWL conditions. No significant changes in lipid, protein, and carbohydrate concentrations were found in the two different light conditions. The results are important for informing on optimum biomass cultivation of this species for biomass production and bioactive product development.

scholarly journals Mycosporine-like amino acid and aromatic amino acid transcriptome response to UV and far-red light in the cyanobacterium Chlorogloeopsis fritschii PCC 6912

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Carole A. Llewellyn ◽  
Carolyn Greig ◽  
Alla Silkina ◽  
Bethan Kultschar ◽  
Matthew D. Hitchings ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gene Cluster ◽  
White Light ◽  
Aromatic Amino Acid ◽  
Uv Light ◽  
Red Light ◽  
Ultra Violet ◽  
Filamentous Cyanobacterium ◽  
Chlorogloeopsis Fritschii ◽  
The Impact

AbstractThe “UV sunscreen” compounds, the mycosporine-like amino acids (MAAs) are widely reported in cyanobacteria and are known to be induced under ultra-violet (UV) light. However, the impact of far red (FR) light on MAA biosynthesis has not been studied. We report results from two experiments measuring transcriptional regulation of MAA and aromatic amino acid pathways in the filamentous cyanobacterium Chlorogloeopsis fritschii PCC 6912. The first experiment, comparing UV with white light, shows the expected upregulation of the characteristic MAA mys gene cluster. The second experiment, comparing FR with white light, shows that three genes of the four mys gene cluster encoding up to mycosporine-glycine are also upregulated under FR light. This is a new discovery. We observed corresponding increases in MAAs under FR light using HPLC analysis. The tryptophan pathway was upregulated under UV, with no change under FR. The tyrosine and phenylalanine pathways were unaltered under both conditions. However, nitrate ABC transporter genes were upregulated under UV and FR light indicating increased nitrogen requirement under both light conditions. The discovery that MAAs are upregulated under FR light supports MAAs playing a role in photon dissipation and thermoregulation with a possible role in contributing to Earth surface temperature regulation.

scholarly journals Response of Key Metabolites during a UV-A Exposure Time-Series in the Cyanobacterium Chlorogloeopsis fritschii PCC 6912

2021 ◽  
Vol 9 (5) ◽  
pp. 910
Author(s):  
Bethan Kultschar ◽  
Ed Dudley ◽  
Steve Wilson ◽  
Carole Anne Llewellyn
Keyword(s):  
Stress Response ◽  
Evolutionary History ◽  
Harmful Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Industrial Biotechnology ◽  
Primary Metabolism ◽  
Ultraviolet A ◽  
Metabolite Level ◽  
Photosynthetic Organisms ◽  
Chlorogloeopsis Fritschii

Ultraviolet A (UV-A) is the major component of UV radiation reaching the Earth’s surface, causing indirect damage to photosynthetic organisms via the production of reactive oxygen species (ROS). In comparison, UV-B causes both direct damage to biomolecules and indirect damage. UV-B is well studied in cyanobacterial research due to their long evolutionary history and adaptation to high levels of UV, with less work on the effects of UV-A. In this study, the response of key metabolites in Chlorogloeopsis fritschii (C. fritschii) during 48 h of photosynthetically active radiation (PAR, 15 µmol·m−2·s−1) supplemented with UV-A (11 µmol·m−2·s−1) was investigated using gas chromatography- mass spectrometry (GC-MS). Results showed an overall significant increase in metabolite levels up to 24 h of UV-A exposure. Compared with previously reported UV-B (PAR + UV-B) and PAR only results, UV-A showed more similarity compared to PAR only exposure as opposed to supplemented UV-B. The amino acids glutamate, phenylalanine and leucine showed differences in levels between UV (both supplemented UV-A and supplemented UV-B) and PAR only (non-supplemented PAR), hinting to their relevance in UV stress response. The fatty acids, palmitic and stearic acid, showed positive log2 fold-change (FC) in supplemented UV-A and PAR only experiments but negative log2 FC in UV-B, indicating the more harmful effect of UV-B on primary metabolism. Less research has been conducted on UV-A exposure and cyanobacteria, a potential environmental stimuli for the optimisation of metabolites for industrial biotechnology. This study will add to the literature and knowledge on UV-A stress response at the metabolite level in cyanobacteria, especially within the less well-known species C. fritschii.

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