Nomenclature Abstract for Sporosarcina ureae (Beijerinck 1901) Kluyver and van Niel 1936 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity ◽  
Dorothea Taylor
Keyword(s):  
Sporosarcina Ureae

scholarly journals Electron Microscopy of Ultrathin Sections of Sporosarcina ureae

1965 ◽  
Vol 90 (3) ◽  
pp. 808-816 ◽  
Author(s):  
K. Mazanec ◽  
M. Kocur ◽  
T. Martinec
Keyword(s):  
Electron Microscopy ◽  
Sporosarcina Ureae ◽  
Ultrathin Sections

scholarly journals The division during bacterial sporulation is symmetrically located in Sporosarcina ureae

1997 ◽  
Vol 25 (6) ◽  
pp. 1091-1098 ◽  
Author(s):  
Ling Zhang ◽  
Michael L. Higgins ◽  
Patrick J. Piggot
Keyword(s):  
Sporosarcina Ureae

scholarly journals Improving the strength of sandy soils via ureolytic CaCO<sub>3</sub> solidification by <i>Sporosarcina ureae</i>

2018 ◽  
Vol 15 (14) ◽  
pp. 4367-4380 ◽  
Author(s):  
Justin Michael Whitaker ◽  
Sai Vanapalli ◽  
Danielle Fortin
Keyword(s):  
Shear Strength ◽  
Acid Rain ◽  
Climatic Conditions ◽  
Carbonate Precipitation ◽  
Biogeochemical Process ◽  
Climate Conditions ◽  
Sporosarcina Ureae ◽  
Microbially Induced Carbonate Precipitation ◽  
Environmental Durability ◽  
Hydrolysis Of

Abstract. “Microbially induced carbonate precipitation” (MICP) is a biogeochemical process that can be applied to strengthen materials. The hydrolysis of urea by microbial catalysis to form carbonate is a commonly studied example of MICP. In this study, Sporosarcina ureae, a ureolytic organism, was compared to other ureolytic and non-ureolytic organisms of Bacillus and Sporosarcina genera in the assessment of its ability to produce carbonates by ureolytic MICP for ground reinforcement. It was found that S. ureae grew optimally in alkaline (pH ∼ 9.0) conditions which favoured MICP and could degrade urea (units U mL−1 represent µmol min−1 mL OD600) at levels (30.28 U mL−1) similar to S. pasteurii (32.76 U mL−1), the model ureolytic MICP organism. When cells of S. ureae were concentrated (OD600 ∼ 15–20) and mixed with cementation medium containing 0.5 M calcium chloride (CaCl2) and urea into a model sand, repeated treatments (3 × 24 h) were able to improve the confined direct shear strength of samples from 15.77 kPa to as much as 135.80 kPa. This was more than any other organism observed in the study. Imaging of the reinforced samples with scanning electron microscopy and energy-dispersive spectroscopy confirmed the successful precipitation of calcium carbonate (CaCO3) across sand particles by S. ureae. Treated samples were also tested experimentally according to model North American climatic conditions to understand the environmental durability of MICP. No statistically significant (p < 0.05, n= 3) difference in strength was observed for samples that underwent freeze–thaw cycling or flood-like simulations. However, shear strength of samples following acid rain simulations fell to 29.2 % of control MICP samples. Overall, the species S. ureae was found to be an excellent organism for MICP by ureolysis to achieve ground strengthening. However, the feasibility of MICP as a durable reinforcement technique is limited by specific climate conditions (i.e. acid rain).

scholarly journals Production, Secretion, and Cell Surface Display of Recombinant Sporosarcina ureae S-Layer Fusion Proteins in Bacillus megaterium

2011 ◽  
Vol 78 (2) ◽  
pp. 560-567 ◽  
Author(s):  
Denise Knobloch ◽  
Kai Ostermann ◽  
Gerhard Rödel
Keyword(s):  
Cell Surface ◽  
Bacillus Megaterium ◽  
Fusion Proteins ◽  
Shuttle Vector ◽  
Surface Display ◽  
Fluorescent Labeling ◽  
Content Type ◽  
Sporosarcina Ureae

ABSTRACTMonomolecular crystalline bacterial cell surface layers (S-layers) have broad application potential in nanobiotechnology due to their ability to generate functional supramolecular structures. Here, we report thatBacillus megateriumis an excellent host organism for the heterologous expression and efficient secretion of hemagglutinin (HA) epitope-tagged versions of the S-layer protein SslA fromSporosarcina ureaeATCC 13881. Three chimeric proteins were constructed, comprising the precursor, C-terminally truncated, and N- and C-terminally truncated forms of the S-layer SslA protein tagged with the human influenza hemagglutinin epitope. For secretion of fusion proteins, the open reading frames were cloned into theEscherichia coli-Bacillus megateriumshuttle vector pHIS1525. After transformation of the respective plasmids intoBacillus megateriumprotoplasts, the recombinant genes were successfully expressed and the proteins were secreted into the growth medium. The isolated S-layer proteins are able to assemblein vitrointo highly ordered, crystalline, sheetlike structures with the fused HA tag accessible to antibody. We further show by fluorescent labeling that the secreted S-layer fusion proteins are also clustered on the cell envelope ofBacillus megaterium, indicating that the cell surface can servein vivoas a nucleation point for crystallization. Thus, this system can be used as a display system that allows the dense and periodic presentation of S-layer proteins or the fused tags.

Deoxyribonucleic acid base composition of Sporosarcina ureae

1968 ◽  
Vol 64 (1) ◽  
pp. 23-28 ◽  
Author(s):  
J. Boháček ◽  
M. Kocur ◽  
T. Martinec
Keyword(s):  
Base Composition ◽  
Deoxyribonucleic Acid ◽  
Acid Base ◽  
Sporosarcina Ureae

scholarly journals Surface arrays on the wall of Sporosarcina ureae.

1979 ◽  
Vol 139 (3) ◽  
pp. 1039-1048 ◽  
Author(s):  
T J Beveridge
Keyword(s):  
Sporosarcina Ureae

Isolation, gene structure, and comparative analysis of the S-layer gene sslA of Sporosarcina ureae ATCC 13881

Genetica ◽  
2007 ◽  
Vol 131 (3) ◽  
pp. 255-265 ◽  
Author(s):  
Pavel M. Ryzhkov ◽  
Kai Ostermann ◽  
Gerhard Rödel
Keyword(s):  
Comparative Analysis ◽  
Gene Structure ◽  
Sporosarcina Ureae
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