Nomenclature Abstract for Clostridium sticklandii Stadtman and McClung 1957 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
George M Garrity
Keyword(s):  
Clostridium Sticklandii

scholarly journals Ornithine Metabolism by Clostridium sticklandii

1970 ◽  
Vol 245 (22) ◽  
pp. 5914-5926
Author(s):  
Yoshihisa Tsuda ◽  
Herbert C. Friedmann
Keyword(s):  
Clostridium Sticklandii

scholarly journals Factors Affecting the Separation and Bioactivity of Red Clover (Trifoliumpratense) Extracts Assayed against Clostridium sticklandii, a Ruminal Hyper Ammonia-producing Bacterium

2012 ◽  
Vol 7 (12) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Isabelle A. Kagan ◽  
Michael D. Flythe
Keyword(s):  
Ethyl Acetate ◽  
Trifolium Pratense ◽  
Red Clover ◽  
Normal Phase ◽  
Biochanin A ◽  
Factors Affecting ◽  
Clostridium Sticklandii ◽  
Tlc Plates ◽  
Phenolic Extract ◽  
Layer Chromatography

Red clover ( Trifolium pratense) is rich in phenolic compounds. Both the crude phenolic extract and biochanin A, an isoflavonoid component of the extract, suppress growth of Clostridium sticklandii, a bovine, Gram-positive, ruminal hyper-ammonia producing bacterium (HAB). The purpose of this study was to determine if other components of red clover extract contributed to its anti-HAB activity. Extracts of the Kenland cultivar of red clover, collected 0 h and 24 h after cutting, were separated by normal-phase thin-layer chromatography (TLC) in either ethyl acetate-hexanes (9:1, v/v) or ethyl acetate-methanol (4:1, v/v). Bands on TLC plates were assayed by either overlaying the plates with agar seeded with C. sticklandii, or setting the excised bands face-down onto plates of bacteria-seeded agar. Biochanin A inhibited C. sticklandii growth on TLC plates when as little as 8 nmol was present in the extract. An antimicrobial band, seen in a previous bioassay, was not found, suggesting that this second compound may be more labile than biochanin A.

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