Nomenclature Abstract for Pantoea stewartii indologenes Mergaert et al. 1993.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity
Keyword(s):  
Pantoea Stewartii

Exemplar Abstract for Pantoea stewartii stewartii (Smith 1898) Mergaert et al. 1993.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Pantoea Stewartii

scholarly journals Pan-Genome of Novel Pantoea stewartii subsp. indologenes Reveals Genes Involved in Onion Pathogenicity and Evidence of Lateral Gene Transfer

2021 ◽  
Vol 9 (8) ◽  
pp. 1761
Author(s):  
Gaurav Agarwal ◽  
Ronald D. Gitaitis ◽  
Bhabesh Dutta
Keyword(s):  
Gene Transfer ◽  
Core Genome ◽  
Foxtail Millet ◽  
Gene Clusters ◽  
Evaluation Study ◽  
Full Spectrum ◽  
Pan Genome ◽  
Pantoea Stewartii ◽  
Comparative Phylogenetic Analysis ◽  
Core Genes

Pantoea stewartii subsp. indologenes (Psi) is a causative agent of leafspot on foxtail millet and pearl millet; however, novel strains were recently identified that are pathogenic on onions. Our recent host range evaluation study identified two pathovars; P. stewartii subsp. indologenes pv. cepacicola pv. nov. and P. stewartii subsp. indologenes pv. setariae pv. nov. that are pathogenic on onions and millets or on millets only, respectively. In the current study, we developed a pan-genome using the whole genome sequencing of newly identified/classified Psi strains from both pathovars [pv. cepacicola (n = 4) and pv. setariae (n = 13)]. The full spectrum of the pan-genome contained 7030 genes. Among these, 3546 (present in genomes of all 17 strains) were the core genes that were a subset of 3682 soft-core genes (present in ≥16 strains). The accessory genome included 1308 shell genes and 2040 cloud genes (present in ≤2 strains). The pan-genome showed a clear linear progression with >6000 genes, suggesting that the pan-genome of Psi is open. Comparative phylogenetic analysis showed differences in phylogenetic clustering of Pantoea spp. using PAVs/wgMLST approach in comparison with core genome SNPs-based phylogeny. Further, we conducted a horizontal gene transfer (HGT) study using Psi strains from both pathovars along with strains from other Pantoea species, namely, P. stewartii subsp. stewartii LMG 2715T, P. ananatis LMG 2665T, P. agglomerans LMG L15, and P. allii LMG 24248T. A total of 317 HGT events among four Pantoea species were identified with most gene transfer events occurring between Psi pv. cepacicola and Psi pv. setariae. Pan-GWAS analysis predicted a total of 154 genes, including seven gene-clusters, which were associated with the pathogenicity phenotype (necrosis on seedling) on onions. One of the gene-clusters contained 11 genes with known functions and was found to be chromosomally located.

scholarly journals The cell density-dependent expression of stewartan exopolysaccharide in Pantoea stewartii ssp. stewartii is a function of EsaR-mediated repression of the rcsA gene

2005 ◽  
Vol 56 (1) ◽  
pp. 189-203 ◽  
Author(s):  
Timothy D. Minogue ◽  
Aurelien L. Carlier ◽  
Maria D. Koutsoudis ◽  
Susanne B. Von Bodman
Keyword(s):  
Cell Density ◽  
Density Dependent ◽  
Pantoea Stewartii

scholarly journals Quantifying the Feeding Periods Required by Corn Flea Beetles to Acquire and Transmit Pantoea stewartii

Plant Disease ◽  
2006 ◽  
Vol 90 (3) ◽  
pp. 319-324 ◽  
Author(s):  
B. Menelas ◽  
C. C. Block ◽  
P. D. Esker ◽  
F. W. Nutter
Keyword(s):  
Nalidixic Acid ◽  
Growth Models ◽  
Flea Beetle ◽  
Feeding Time ◽  
Feeding Period ◽  
Selective Media ◽  
Pantoea Stewartii ◽  
Flea Beetles ◽  
Time Required ◽  
The Relationship

The feeding periods required by corn flea beetles to acquire and transmit Pantoea stewartii were investigated in the Stewart's disease of corn pathosystem. To quantify the effect of acquisition feeding period on percentage of acquisition, field-collected corn beetles were allowed to feed for 6, 12, 24 36, 48, and 72 h on corn seedlings previously inoculated with a rifampicin- and nalidixic acid-restraint strain of P. stewartii. Acquisition of P. stewartii by corn flea beetles was considered positive if the rifampicin- and nalidixic acid-marked strain was recovered on selective media. To quantity the effect of transmission feeding period on percent transmission of P. stewartii by corn flea beetles, P. stewartii- infested corn flea beetles were allowed to feed on healthy corn seedlings for periods of 3, 6, 12, 24, 36, 48, and 72 h. After the appropriate transmission feeding period, leaf tissues surrounding the sites of feeding scars were cultured for the presence of the P. stewartii-marked strain. Transmission of P. stewartii was considered positive if the marked strain was recovered on selective media. Acquisition of P. stewartii occurred within 6 h and the percentage of corn flea beetles that had acquired P. stewartii after 72 h ranged from 68 to 94%. The change in P. stewartii acquisition by corn flea beetles (Y) with respect to acquisition feeding period (X) was best described by the Gompertz model, with R2 values ranging from 91 to 99%. The mean time for acquisition by 50% of the corn flea beetles was 36.5 ± 11.6 h. The minimum transmission feeding time required for corn flea beetles to transmit P. stewartii following a 48-h acquisition feeding period was less than 3 h. The percent transmission of P. stewartii by corn flea beetles was nearly 100% after a 48-h transmission feeding period and was 100% by 72 h. Among population growth models evaluated, the monomolecular model best described the relationship between percent transmission (Y) and transmission feeding periods (X), with R 2 values of up to 84%. However, a nonlinear form of the monomolecular model better quantified the relationship between percent transmission and transmission feeding period, because pseudo-R2 values ranged between 98.1 and 99.5%. The predicted transmission feeding time required for 50% of P. stewartii-infested corn flea beetles to transmit the pathogen was 7.6 ± 0.87 h. These results suggest that the corn flea beetle is a highly efficient vector that can quickly acquire and transmit P. stewartii, thereby requiring insecticide seed treatments and foliar insecticides that act quickly to prevent corn flea beetles from acquiring and transmitting P. stewartii to corn plants.

scholarly journals Pest survey card on Pantoea stewartii subsp. stewartii

2020 ◽  
Vol 17 (6) ◽  
Author(s):  
◽  
Dirk Jan van der Gaag ◽  
Martijn Schenk ◽  
Alice Delbianco ◽  
Sybren Vos
Keyword(s):  
Pantoea Stewartii

First Report of Pantoea stewartii subsp. indologenes Causing Leaf Blight on Rice in Malaysia

Plant Disease ◽  
2019 ◽  
Vol 103 (6) ◽  
pp. 1407-1407 ◽  
Author(s):  
M. M. F. Azizi ◽  
S. I. Ismail ◽  
E. M. Hata ◽  
D. Zulperi ◽  
M. Y. Ina-Salwany ◽  
...  
Keyword(s):  
Leaf Blight ◽  
First Report ◽  
Pantoea Stewartii

scholarly journals A Sequence That Affects the Copy Number and Stability of pSW200 and ColE1

2010 ◽  
Vol 192 (14) ◽  
pp. 3654-3660 ◽  
Author(s):  
Ying-Chung Wu ◽  
Shih-Tung Liu
Keyword(s):  
Copy Number ◽  
Plasmid Stability ◽  
Efficient Synthesis ◽  
Transcriptional Fusion ◽  
Similar Sequence ◽  
Pantoea Stewartii ◽  
The Stability

ABSTRACT Pantoea stewartii SW2 contains 13 plasmids. One of these plasmids, pSW200, has a replicon that resembles that of ColE1. This study demonstrates that pSW200 contains a 9-bp UP element, 5′-AAGATCTTC, which is located immediately upstream of the −35 box in the RNAII promoter. A transcriptional fusion study reveals that substituting this 9-bp sequence reduces the activity of the RNAII promoter by 78%. The same mutation also reduced the number of plasmid copies from 13 to 5, as well as the plasmid stability. When a similar sequence in a ColE1 derivative, pYCW301, is mutated, the copy number of the plasmid also declines from 34 to 16 per cell. Additionally, inserting this 9-bp sequence stabilizes an unstable pSW100 derivative, pSW142K, which also contains a replicon resembling that of ColE1, indicating the importance of this sequence in maintaining the stability of the plasmid. In conclusion, the 9-bp sequence upstream of the −35 box in the RNAII promoter is required for the efficient synthesis of RNAII and maintenance of the stability of the plasmids in the ColE1 family.

scholarly journals Detection and Visualization of an Exopolysaccharide Produced by Xylella fastidiosa In Vitro and In Planta

2007 ◽  
Vol 73 (22) ◽  
pp. 7252-7258 ◽  
Author(s):  
M. Caroline Roper ◽  
L. Carl Greve ◽  
John M. Labavitch ◽  
Bruce C. Kirkpatrick
Keyword(s):  
Xanthan Gum ◽  
Xanthomonas Campestris ◽  
Polyclonal Antibodies ◽  
Xylella Fastidiosa ◽  
Enzyme Linked Immunosorbent Assay ◽  
In Planta ◽  
Mannose Residue ◽  
Pantoea Stewartii ◽  
Vascular Occlusions

ABSTRACT Many phytopathogenic bacteria, such as Ralstonia solanacearum, Pantoea stewartii, and Xanthomonas campestris, produce exopolysaccharides (EPSs) that aid in virulence, colonization, and survival. EPS can also contribute to host xylem vessel blockage. The genome of Xylella fastidiosa, the causal agent of Pierce's disease (PD) of grapevine, contains an operon that is strikingly similar to the X. campestris gum operon, which is responsible for the production of xanthan gum. Based on this information, it has been hypothesized that X. fastidiosa is capable of producing an EPS similar in structure and composition to xanthan gum but lacking the terminal mannose residue. In this study, we raised polyclonal antibodies against a modified xanthan gum polymer similar to the predicted X. fastidiosa EPS polymer. We used enzyme-linked immunosorbent assay to quantify production of EPS from X. fastidiosa cells grown in vitro and immunolocalization microscopy to examine the distribution of X. fastidiosa EPS in biofilms formed in vitro and in planta and assessed the contribution of X. fastidiosa EPS to the vascular occlusions seen in PD-infected grapevines.

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