Nomenclature Abstract for Erwinia stewartii (Smith 1898) Dye 1963 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity ◽  
Dorothea Taylor
Keyword(s):  
Erwinia Stewartii

Cloning and expression of Xylella fastidiosa antigens in Escherichia coli and Erwinia stewartii

1989 ◽  
Vol 35 (4) ◽  
pp. 487-491 ◽  
Author(s):  
Paul H. Goodwin
Keyword(s):  
Escherichia Coli ◽  
Molecular Weight ◽  
Genomic Dna ◽  
Xylella Fastidiosa ◽  
Gene Bank ◽  
Cloning And Expression ◽  
Western Blots ◽  
E Coli ◽  
Cosmid Vector ◽  
Erwinia Stewartii

Xylella fastidiosa DNA, partially digested with Sau3A, was ligated into the cosmid vector, pUCD615. Approximately 4500 ampicillin-resistant Escherichia coli colonies were obtained. The frequency of complementation of leucine auxotrophy in transfected E. coli indicated that the cosmid gene bank was representative of X. fastidiosa genomic DNA. Colonies were lysed directly onto nitrocellulose membranes using a thermo-inducible λ lysogen and screened for expression of X. fastidiosa antigens. Approximately 16.5% of a random sample of clones were found to express X. fastidiosa antigens as determined by Western blots. These proteins comigrated with proteins of X. fastidiosa and ranged in molecular weight from 10 000 to 160 000. Conjugation of several of the plasmids into Erwinia stewartii resulted in expression of the similar molecular weight cloned proteins with similar levels of expression as in E. coli.Key words: Xylella fastidiosa, Pierce's disease, immunological clone screening, thermo-inducible lysogeny.

Induction of avirulent variants in Erwinia stewartii by incubation at supraoptimal temperatures

1975 ◽  
Vol 21 (8) ◽  
pp. 1282-1287 ◽  
Author(s):  
A. Garibaldi ◽  
L. N. Gibbins
Keyword(s):  
Zea Mays ◽  
High Temperatures ◽  
Parent Strain ◽  
Small Colony Variants ◽  
Supraoptimal Temperatures ◽  
Small Colony ◽  
Erwinia Stewartii

High temperatures (37 °C) induced non-pigmented, and (or) small colony variants in some Erwinia stewartii strains. The former differed from the parent strain serologically and in having lost virulence to Zea mays. The small colony variants retained phytopathogenicity.

Structural and functional analysis of the rcsA gene from Erwinia stewartii

1991 ◽  
Vol 229 (1) ◽  
pp. 155-160 ◽  
Author(s):  
Karl Poetter ◽  
David L. Coplin
Keyword(s):  
Functional Analysis ◽  
Erwinia Stewartii

Characterization of a gene locus from Erwinia amylovora with regulatory functions in exopolysaccharide synthesis of Erwinia spp.

1998 ◽  
Vol 44 (7) ◽  
pp. 657-666 ◽  
Author(s):  
Phillip Aldridge ◽  
Frank Bernhard ◽  
Peter Bugert ◽  
David L Coplin ◽  
Klaus Geider
Keyword(s):  
Structural Gene ◽  
Erwinia Amylovora ◽  
Genomic Library ◽  
Expression Vectors ◽  
External Signal ◽  
Wild Type ◽  
Reading Frame ◽  
Gus Reporter ◽  
Acid Protein ◽  
Erwinia Stewartii

In a genomic library of Erwinia amylovora, a locus has been identified that can suppress an Erwinia stewartii rcsA mutant. In addition, the locus induced a mucoid sticky phenotype of colonies in a wild-type strain of Erwinia stewartii and increased exopolysaccharide synthesis in several species of bacteria belonging to the genus Erwinia. An open reading frame was identified at this locus encoding a 225 amino acid protein that contained a helix-turn-helix motif typical of transcriptional regulators. The corresponding gene was subsequently named rcsV (regulator of capsular synthesis affecting viscosity). A mutant of rcsV in wild-type Erwinia amylovora had no detectable phenotype and produced typical levels of amylovoran under laboratory conditions. The rcsV gene on a high copy number plasmid under the control of its own promoter did not alter amylovoran production, in contrast to in-frame fusions of the structural gene in expression vectors. Since even the lac promoter was inert in the expression of rcsV, a DNA-binding protein could inhibit transcription of the gene in Erwinia amylovora. On the other hand, an Erwinia amylovora rcsA mutant was suppressed by rcsV when its promoter was replaced and the structural gene fused in-frame with lacZ' or malE. Northern blots, with total RNA from Erwinia amylovora, or promoter analysis using the GUS reporter gene did not show expression of rcsV in Erwinia amylovora, although primer extension analysis did. RcsV could be a component involved in the regulation of amylovoran synthesis, and gene expression may require an unknown external signal during the life cycle or pathogenesis of Erwinia amylovora. Key words: amylovoran, fire blight, rcsA-like activator, fusion protein.

scholarly journals Ability of an ELISA-Based Seed Health Test to Detect Erwinia stewartii in Maize Seed Treated with Fungicides and Insecticides

Plant Disease ◽  
2004 ◽  
Vol 88 (6) ◽  
pp. 633-640 ◽  
Author(s):  
J. K. Pataky ◽  
C. C. Block ◽  
P. M. Michener ◽  
L. M. Shepherd ◽  
D. C. McGee ◽  
...  
Keyword(s):  
Seed Treatment ◽  
Type I Error ◽  
Enzyme Linked Immunosorbent Assay ◽  
Type I ◽  
Maize Seed ◽  
Seed Treatments ◽  
Seed Health ◽  
Erwinia Stewartii ◽  
Seed Health Test ◽  
Health Test

Two sets of experiments were done to examine whether seed-treatment chemicals affected the ability of an enzyme-linked immunosorbent assay (ELISA)-based seed health test to detect Erwinia stewartii. The chemicals evaluated included Actellic, Apron, Captan, Cruiser, Gaucho, Maxim, Poncho, Thiram, and Vitavax in 11 seed-treatment combinations. In one experiment, seed-treatment chemicals were evaluated quantitatively in a critical region of ELISA absorbance values near 0.5 using maize seed that were spiked with uniform quantities of a liquid suspension of E. stewartii. The number of bacteria in each sample was estimated from ELISA absorbance values using standard curves. Log CFU of E. stewartii per sample were not significantly different among the untreated control and the 11 seed treatments compared with Tukey's Studentized Range Test (P = 0.05). Means of log CFU/ml for all treatments were tightly clustered around 5.70 which corresponded to an absorbance value of 0.440 and a bacterial population of about 500,000 CFU/ml. In a second set of experiments, seed treatment chemicals were evaluated based on qualitative decisions that resulted from the ELISA-based seed health test of seed lots of Jubilee and A632 infected with E. stewartii. The number of negative samples was not substantially greater than expected based on binomial probabilities except for samples of Captan/Vitavax-treated A632, which we considered to be a type I error. The mean absorbance values of positive samples ranged from 1.42 to 1.72 for A632 and from 1.51 to 1.91 for Jubilee and did not differ significantly among the seed treatments. There was no consistent evidence from these experiments that fungicide or insecticide seed treatments interfered with the sensitivity of the ELISA or altered low (e.g., 0.5) or high (e.g. 1.4 to 1.9) absorbance values. The ability of the ELISA-based seed health test to detect E. stewartii in maize seed was not affected by these seed treatments.

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