Nomenclature Abstract for Rhizobium fredii Scholla and Elkan 1984.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity
Keyword(s):  
Rhizobium Fredii

scholarly journals Characterization of the Overlapping Promoters of nolB and nolW, Two Soybean Cultivar Specificity Genes from Rhizobium fredii Strain USDA257

1997 ◽  
Vol 10 (1) ◽  
pp. 138-141 ◽  
Author(s):  
Jun Gu ◽  
Pedro A. Balatti ◽  
Hari B. Krishnan ◽  
Steven G. Pueppke
Keyword(s):  
Regulatory Gene ◽  
Initiation Site ◽  
Dual Control ◽  
Start Site ◽  
Rhizobium Fredii ◽  
Normal Expression ◽  
Transcript Initiation ◽  
Cultivar Specificity ◽  
Transcript Start Site

The transcripts of nolW and nolB, two divergently oriented cultivar specificity genes of Rhizobium fredii strain USDA257, are known to be initiated 14 bp apart from promoters that face one another. We show here that expression of nolB is dependent both on induction with flavonoid signals and on the regulatory gene, nodD1. Expression of nolW is constitutive and independent of flavonoids and nodD1. Normal expression of nolB is retained with a promoter that extends only 61 bp upstream of the transcript start site, but it is lost if an additional 24 bp are removed. Substantial expression of nolW is retained with a promoter that contains only 34 bp of DNA upstream from the transcript initiation site. The dual control region for the two genes is thus only about 109 bp in length.

Transcriptional organization and expression of noIXWBTUV, a locus that regulates cultivar-specific nodulatlon of soybean by Rhizobium fredii USDA257

1995 ◽  
Vol 17 (5) ◽  
pp. 923-933 ◽  
Author(s):  
Laszlo G. Kovacs ◽  
Pedro A. Balatti ◽  
Hari B. Krishnan ◽  
Steven G. Pueppke
Keyword(s):  
Rhizobium Fredii

Cloning and Expression in Pseudomonas putida of Two of the Histidine Utilization Genes from Rhizobium fredii

1997 ◽  
Vol 34 (1) ◽  
pp. 55-60
Author(s):  
Robert R. Burke ◽  
Diana D. Heard ◽  
Anthony J. Nieuwkoop
Keyword(s):  
Pseudomonas Putida ◽  
Cloning And Expression ◽  
Rhizobium Fredii

Nod Factors from Rhizobium Fredii USDA257

1993 ◽  
pp. 157-158 ◽  
Author(s):  
M. P. Bec-Ferté ◽  
A. Savagnac ◽  
S. G. Pueppke ◽  
J. C. Promé
Keyword(s):  
Nod Factors ◽  
Rhizobium Fredii

Nodulation competitiveness of Tn5-induced mutants of Rhizobium fredii USDA208 that are altered in motility and extracellular polysaccharide production

1991 ◽  
Vol 37 (1) ◽  
pp. 52-58 ◽  
Author(s):  
Robert E. Zdor ◽  
Steven G. Pueppke
Keyword(s):  
Parental Strain ◽  
Extracellular Polysaccharide ◽  
Triphenyltetrazolium Chloride ◽  
Polysaccharide Production ◽  
Production Type ◽  
Rhizobium Fredii ◽  
Induced Mutants ◽  
Nodulation Competitiveness

The role of motility and extracellular polysaccharide production in nodulation competitiveness of Rhizobium fredii was examined. Transposon Tn5 was used to mutagenize strain USDA208, and mutants with reduced motility on semisolid agar medium were isolated. One such mutant, 208M3, migrated to only one-seventh the distance of the parental strain. Solid medium amended with triphenyltetrazolium chloride was used to identify mutants altered in extracellular polysaccharide production. Type 1 colonies, typified by mutant 208T13, were heavily mucoid, while type 2 colonies, represented by mutant 208T3, were dry and nonmucoid. Compared with strain USDA208, these mutants produced 4- to 5-fold more extracellular polysaccharide and 20% as much extracellular polysaccharide, respectively. Marker exchange of 208T3 genomic DNA containing Tn5 into strain USDA208 resulted in a mutant, 208K1, that produced extracellular polysaccharide levels similar to mutant 208T3. Mutants 208M3, 208T3, and 208T13 contained single Tn5 insertions. All formed pink nodules on 'Peking' soybean that were structurally indistinguishable and contained proteins with similar profiles. Rates of nodulation were similar in the mutants and the parental strain. Mutants 208M3 and 208T13 were as competitive against an isolate of Bradyrhizobium japonicum serogroup 123 as was strain USDA208. In contrast, mutants 208T3 and 208K1 were competitively superior. Key words: nodulation competition, motility, extracellular polysaccharide, Rhizobium.

scholarly journals Molecular cloning and expression of Rhizobium fredii USDA 193 nodulation genes: extension of host range for nodulation.

1986 ◽  
Vol 168 (3) ◽  
pp. 1087-1095 ◽  
Author(s):  
N Ramakrishnan ◽  
R K Prakash ◽  
S Shantharam ◽  
N M Duteau ◽  
A G Atherly
Keyword(s):  
Host Range ◽  
Molecular Cloning ◽  
Cloning And Expression ◽  
Nodulation Genes ◽  
Rhizobium Fredii
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