Exemplar Abstract for Serpula hyodysenteriae (Harris et al. 1972) Stanton et al. 1991, Brachyspira hyodysenteriae (Stanton 1992) Ochiai et al. 1998 emend. Hördt et al. 2020, Serpulina hyodysenteriae Stanton 1992 pro synon. Serpula hyodysenteriae (Harris et al. 1972) Stanton et al. 1991 and Treponema hyodysenteriae Harris et al. 1972 (Approved Lists 1980).

Characterization of a Periplasmic ATP-Binding Cassette Iron Import System of Brachyspira(Serpulina) hyodysenteriae

Journal of Bacteriology ◽

10.1128/jb.181.22.6948-6957.1999 ◽

1999 ◽

Vol 181 (22) ◽

pp. 6948-6957 ◽

Cited By ~ 21

Author(s):

Dominique Dugourd ◽

Christine Martin ◽

Clément R. Rioux ◽

Mario Jacques ◽

Josée Harel

Keyword(s):

Binding Proteins ◽

Genomic Region ◽

Open Reading Frames ◽

Atp Binding ◽

Binding Motifs ◽

Brachyspira Hyodysenteriae ◽

Serpulina Hyodysenteriae ◽

Periplasmic Binding Proteins ◽

Atp Binding Cassette

ABSTRACT The nucleotide sequence of the pathogenic spirocheteBrachyspira hyodysenteriae bit (for “Brachyspira iron transport”) genomic region has been determined. The bit region is likely to encode an iron ATP-binding cassette transport system with some homology to those encountered in gram-negative bacteria. Six open reading frames oriented in the same direction and physically linked have been identified. This system possesses a protein containing ATP-binding motifs (BitD), two hydrophobic cytoplasmic membrane permeases (BitE and BitF), and at least three lipoproteins (BitA, BitB, and BitC) with homology to iron periplasmic binding proteins. These periplasmic binding proteins exhibit lipoprotein features. They are labeled by [3H]palmitate when tested in recombinantEscherichia coli, and their signal peptides are typical for substrates of the type II secretory peptidase. The FURTA system and Congo red assay indicate that BitB and BitC are involved in iron binding. The Bit system is detected only in B. hyodysenteriae and is absent from B. innocens andB. pilosicoli.

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Susceptibility to pleuromutilins inBrachyspira(Serpulina)hyodysenteriae

Animal Health Research Reviews ◽

10.1079/ahrr200118 ◽

2001 ◽

Vol 2 (1) ◽

pp. 59-66 ◽

Cited By ~ 33

Author(s):

M. Karlsson ◽

A. Gunnarsson ◽

A. Franklin

Keyword(s):

Minimum Inhibitory Concentration ◽

Antimicrobial Agents ◽

Inhibitory Concentration ◽

The Other ◽

Cross Resistance ◽

Swine Dysentery ◽

Brachyspira Hyodysenteriae ◽

Serpulina Hyodysenteriae ◽

Broth Dilution

AbstractThe pleuromutilins are the only antimicrobial agents with sufficient minimum inhibitory concentration (MIC) values left to treat swine dysentery in Sweden. Other antimicrobials are either not approved for use against swine dysentery or only partly active againstBrachyspira hyodysenteriae. To date, in Sweden two pleuromutilins, tiamulin and valnemulin, are authorized for use in pigs. This study includes a comparison between MICs of tiamulin and valnemulin for Swedish field isolates ofB. hyodysenteriae, as determined by broth dilution. For different isolates the MIC of tiamulin was between 0 and 8 times higher than that of valnemulin. No resistance to pleuromutilins was recorded (tiamulin MIC range 0.031–2 μg/ml, valnemulin MIC range ≤0.016–1 μg/ml).In vitrodevelopment of tiamulin resistance was also studied. TwoB. hyodysenteriaeand twoB. pilosicolistrains became resistant to tiamulin following reiterated passages on agar containing tiamulin in increasing concentrations. The resistance emerged slowly and three of the strains that went through more than 60 passages increased their tiamulin MICs from 0.031–0.25 to more than 128 μg/ml. The tiamulin MIC for oneB. hyodysenteriaestrain that went through 29 passages increased from 0.0125 to 4 μg/ml. OneB. pilosicolistrain developed cross-resistance to valnemulin; the MIC increased from 0.25 to more than 64 μg/ml. The valnemulin MIC for oneB. hyodysenteriaestrain increased from 0.031 μg/ml to 32 μg/ml. Valnemulin MIC was not determined for theB. hyodysenteriaestrain that only went through 29 passages. The valnemulin MIC of the otherB. pilosicolistrain increased from 0.031 to 4 μg/ml.

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