Exemplar Abstract for Leuconostoc lactis Garvie 1960 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Leuconostoc Lactis

scholarly journals Development of Innovative Pediocin PA-1 by DNA Shuffling among Class IIa Bacteriocins

2007 ◽  
Vol 73 (16) ◽  
pp. 5292-5299 ◽  
Author(s):  
Tatsuya Tominaga ◽  
Yoshinori Hatakeyama
Keyword(s):  
Dairy Products ◽  
Dairy Product ◽  
Food Additives ◽  
Dna Shuffling ◽  
Activity Assay ◽  
Effective Version ◽  
Activity Screening ◽  
Leuconostoc Lactis ◽  
Enterocin A ◽  
Increased Activity

ABSTRACT Pediocin PA-1 is a member of the class IIa bacteriocins, which show antimicrobial effects against lactic acid bacteria. To develop an improved version of pediocin PA-1, reciprocal chimeras between pediocin PA-1 and enterocin A, another class IIa bacteriocin, were constructed. Chimera EP, which consisted of the C-terminal half of pediocin PA-1 fused to the N-terminal half of enterocin A, showed increased activity against a strain of Leuconostoc lactis isolated from a sour-spoiled dairy product. To develop an even more effective version of this chimera, a DNA-shuffling library was constructed, wherein four specific regions within the N-terminal half of pediocin PA-1 were shuffled with the corresponding sequences from 10 other class IIa bacteriocins. Activity screening indicated that 63 out of 280 shuffled mutants had antimicrobial activity. A colony overlay activity assay showed that one of the mutants (designated B1) produced a >7.8-mm growth inhibition circle on L. lactis, whereas the parent pediocin PA-1 did not produce any circle. Furthermore, the active shuffled mutants showed increased activity against various species of Lactobacillus, Pediococcus, and Carnobacterium. Sequence analysis revealed that the active mutants had novel N-terminal sequences; in active mutant B1, for example, the parental pediocin PA-1 sequence (KYYGNGVTCGKHSC) was changed to TKYYGNGVSCTKSGC. These new and improved DNA-shuffled bacteriocins could prove useful as food additives for inhibiting sour spoilage of dairy products.

scholarly journals Different Modes of Regulation of the Expression of Dextransucrase in Leuconostoc lactis AV1n and Lactobacillus sakei MN1

2019 ◽  
Vol 10 ◽  
Author(s):  
Norhane Besrour-Aouam ◽  
Maria Luz Mohedano ◽  
Imene Fhoula ◽  
Kenza Zarour ◽  
Afef Najjari ◽  
...  
Keyword(s):  
Lactobacillus Sakei ◽  
Leuconostoc Lactis

scholarly journals 686. Evaluation of Contezolid Activity to Anaerobic and Gram-positive-cocci Isolates from a Phase 3 Acute Bacterial Skin and Skin Structure Infection Clinical Trial (MRX-I-06)

2019 ◽  
Vol 6 (Supplement_2) ◽  
pp. S312-S312
Author(s):  
Yang Yang ◽  
Demei Zhu
Keyword(s):  
Dilution Method ◽  
Phase 3 ◽  
Gram Positive ◽  
In Vitro Susceptibility ◽  
Skin Structure ◽  
Prevotella Bivia ◽  
In Vitro Antibacterial Activity ◽  
Agar Dilution ◽  
Leuconostoc Lactis

Abstract Background Contezolid (MRX-I) is an oxazolidinone in development for the treatment of acute bacterial skin and skin structure infections (ABSSSIs). In this study, in vitro susceptibility (S) for Contezolid and comparator agents for Gram-positive (GP) and anaerobic isolates from Phase 3 ABSSSI clinical trials were determined. Methods 313 isolates were collected from 65 participated sites and sent to a central laboratory for MIC testing. Clinical isolates included 34 anaerobes (15 Finegoldia magna, 8 Actinomyces spp., 4 Prevotella spp., 3 Propionibacterium avidum, 2 Peptostreptococcus spp., 1 Veillonella spp. and 1 Bacteroides fragilis), 187 S. aureus (59.7%). 12 S. pyogenes, 5 Enterococcus, and 75 other Gram-positive organisms. Broth micro-dilution method was used to determine the MIC of contezolid, linezolid, and other comparators to facultative isolates. Agar dilution was carried out for the anaerobes. Results For both 33 MRSA and 154 MSSA MIC50/90 values of contezolid and linezolid were 2 mg/L. One E. faecalis showed decreased susceptibility to oxazolidinones (both MIC = 4). 1 mg/L contezolid and linezolid could inhibit 12 S. pyogenes. 2 mg/L contezolid and linezolid could inhibit 15 Finegoldia magna. 0.5 mg/L contezolid and linezolid could inhibit 8 Actinomyces spp. To one Bacteroides fragili, two Prevotella bivia and one Leuconostoc lactis (Intrinsic resistant to vancomycin) the MIC of contezolid were 4 or 8 mg/L. In general, Contezolid had lower or equal MIC50/90 values against both GP and ANA species compared with linezolid for all organisms. Conclusion Contezolid demonstrated potent in vitro antibacterial activity against Gram-positive and anaerobic isolates tested. These data suggest that contezolid might be a beneficial supplement to the arena against MDR Gram-positive infection. Disclosures All authors: No reported disclosures.

scholarly journals Impact of a Complex Food Microbiota on Energy Metabolism in the Model OrganismCaenorhabditis elegans

2015 ◽  
Vol 2015 ◽  
pp. 1-12 ◽  
Author(s):  
Elena Zanni ◽  
Chiara Laudenzi ◽  
Emily Schifano ◽  
Claudio Palleschi ◽  
Giuditta Perozzi ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Model System ◽  
Lactobacillus Delbrueckii ◽  
Altered Expression ◽  
C Elegans ◽  
Gut Colonization ◽  
Evolutionarily Conserved ◽  
Before And After ◽  
Leuconostoc Lactis ◽  
The Impact

The nematodeCaenorhabditis elegansis widely used as a model system for research on aging, development, and host-pathogen interactions. Little is currently known about the mechanisms underlying the effects exerted by foodborne microbes. We took advantage ofC. elegansto evaluate the impact of foodborne microbiota on well characterized physiological features of the worms. Foodborne lactic acid bacteria (LAB) consortium was used to feed nematodes and its composition was evaluated by 16S rDNA analysis and strain typing before and after colonization of the nematode gut.Lactobacillus delbrueckii, L. fermentum, andLeuconostoc lactiswere identified as the main species and shown to display different worm gut colonization capacities. LAB supplementation appeared to decrease nematode lifespan compared to the animals fed with the conventionalEscherichia colinutrient source or a probiotic bacterial strain. Reduced brood size was also observed in microbiota-fed nematodes. Moreover, massive accumulation of lipid droplets was revealed by BODIPY staining. Altered expression ofnhr-49, pept-1, and tub-1genes, associated with obesity phenotypes, was demonstrated by RT-qPCR. Since several pathways are evolutionarily conserved inC. elegans, our results highlight the nematode as a valuable model system to investigate the effects of a complex microbial consortium on host energy metabolism.

Xylooligosaccharide fermentation with Leuconostoc lactis

2006 ◽  
Vol 101 (5) ◽  
pp. 415-420 ◽  
Author(s):  
Hitomi Ohara ◽  
Michiko Owaki ◽  
Kenji Sonomoto
Keyword(s):  
Leuconostoc Lactis

scholarly journals Purification and characterization of a novel glucansucrase from Leuconostoc lactis EG001

2010 ◽  
Vol 165 (5) ◽  
pp. 384-391 ◽  
Author(s):  
Yong-Mo Kim ◽  
Min Ji Yeon ◽  
Nack-Shick Choi ◽  
Young-Hyo Chang ◽  
Min Young Jung ◽  
...  
Keyword(s):  
Purification And Characterization ◽  
Leuconostoc Lactis
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