Exemplar Abstract for Pseudoalteromonas rubra (Gauthier 1976) Gauthier et al. 1995 and Alteromonas rubra Gauthier 1976 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Nicole Danielle Osier ◽  
George M Garrity
Keyword(s):  
Pseudoalteromonas Rubra

scholarly journals Optimising the DPPH Assay for Cell-Free Marine Microorganism Supernatants

Marine Drugs ◽  
2021 ◽  
Vol 19 (5) ◽  
pp. 256
Author(s):  
Yehui Gang ◽  
Tae-Yang Eom ◽  
Svini Dileepa Marasinghe ◽  
Youngdeuk Lee ◽  
Eunyoung Jo ◽  
...  
Keyword(s):  
Water Content ◽  
Antioxidant Activities ◽  
Fermented Foods ◽  
Solution Stability ◽  
Marine Microorganism ◽  
Marine Microorganisms ◽  
Dpph Assay ◽  
Aqueous Methanol ◽  
Potential Applications ◽  
Pseudoalteromonas Rubra

Antioxidants prevent ageing and are usually quantified and screened using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. However, this assay cannot be used for salt-containing samples, such as the cell-free supernatants of marine microorganisms that are aggregated under these conditions. Herein, the DPPH solvent (methanol or ethanol) and its water content were optimized to enable the analysis of salt-containing samples, aggregation was observed for alcohol contents of >70%. The water content of methanol influenced the activities of standard antioxidants but did not significantly affect that of the samples. Based on solution stability considerations, 70% aqueous methanol was chosen as the optimal DPPH solvent. The developed method was successfully applied to the cell-free supernatants of marine bacteria (Pseudoalteromonas rubra and Pseudoalteromonas xiamenensis), revealing their high antioxidant activities. Furthermore, it was concluded that this method would be useful for the screening of marine microorganism–derived antioxidants, which also has numerous potential applications, such as salt-fermented foods.

scholarly journals Chitin Degradation Machinery and Secondary Metabolite Profiles in the Marine Bacterium Pseudoalteromonas rubra S4059

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 108
Author(s):  
Xiyan Wang ◽  
Thomas Isbrandt ◽  
Mikael Lenz Strube ◽  
Sara Skøtt Paulsen ◽  
Maike Wennekers Nielsen ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Genetic Manipulation ◽  
Genome Mining ◽  
Hydrolytic Enzymes ◽  
Metabolite Profile ◽  
Chitinolytic Enzyme ◽  
Chitin Degradation ◽  
Metabolite Profiles ◽  
Bioactive Potential ◽  
Pseudoalteromonas Rubra

Genome mining of pigmented Pseudoalteromonas has revealed a large potential for the production of bioactive compounds and hydrolytic enzymes. The purpose of the present study was to explore this bioactivity potential in a potent antibiotic and enzyme producer, Pseudoalteromonas rubra strain S4059. Proteomic analyses (data are available via ProteomeXchange with identifier PXD023249) indicated that a highly efficient chitin degradation machinery was present in the red-pigmented P. rubra S4059 when grown on chitin. Four GH18 chitinases and two GH20 hexosaminidases were significantly upregulated under these conditions. GH19 chitinases, which are not common in bacteria, are consistently found in pigmented Pseudoalteromonas, and in S4059, GH19 was only detected when the bacterium was grown on chitin. To explore the possible role of GH19 in pigmented Pseudoalteromonas, we developed a protocol for genetic manipulation of S4059 and deleted the GH19 chitinase, and compared phenotypes of the mutant and wild type. However, none of the chitin degrading ability, secondary metabolite profile, or biofilm-forming capacity was affected by GH19 deletion. In conclusion, we developed a genetic manipulation protocol that can be used to unravel the bioactive potential of pigmented pseudoalteromonads. An efficient chitinolytic enzyme cocktail was identified in S4059, suggesting that this strain could be a candidate with industrial potential.

The structure of the O-polysaccharide of the Pseudoalteromonas rubra ATCC 29570T lipopolysaccharide containing a keto sugar

2005 ◽  
Vol 340 (15) ◽  
pp. 2369-2375 ◽  
Author(s):  
Michelle Kilcoyne ◽  
Alexander S. Shashkov ◽  
Yuriy A. Knirel ◽  
Raisa P. Gorshkova ◽  
Evgeny L. Nazarenko ◽  
...  
Keyword(s):  
Pseudoalteromonas Rubra

scholarly journals Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e8093
Author(s):  
Rhesi Kristiana ◽  
Gilles Bedoux ◽  
Gerard Pals ◽  
I. Wayan Mudianta ◽  
Laure Taupin ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Vero Cells ◽  
Bacterial Symbionts ◽  
Cytotoxic Effects ◽  
Activity Screening ◽  
Simplex Virus ◽  
Screening Activity ◽  
Pseudoalteromonas Rubra ◽  
Hsv 1

The aims of this work are to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-Herpes simplex virus type 1 (HSV-1) activities of bio compounds. A total of 15 species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated two active bacteria. Ethyl acetate extracts from supernatants, indicating extracelullar compounds, showed an inhibition zone against MRSA at concentrations of 500–1,000 µg/ml. DNA sequence analysis showed that the strain KJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, whereas the strain NP31-01 isolated from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of Pseudoalteromonas rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5–1,000 µg/ml. No anti HSV-1 was observed for both isolated bacteria. This is the first study describing research on anti-MRSA, cytotoxicity and anti HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced by Pseudoalteromonas rubra and V. salarius, had potential anti-MRSA activity. However, only extracts from Pseudoalteromonas rubra showed cytotoxic effects on Vero cells. Three compounds were identified by LC/MS after purification from culture supernatant.

scholarly journals Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

2019 ◽  
Author(s):  
Rhesi Kristiana ◽  
Gilles Bedoux ◽  
Gerard Pals ◽  
I Wayan Mudianta ◽  
Laure Taupin ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Vero Cells ◽  
Bacterial Symbionts ◽  
Cytotoxic Effects ◽  
Reported Study ◽  
Activity Screening ◽  
Screening Activity ◽  
Pseudoalteromonas Rubra ◽  
Ethyl Acetate Extracts ◽  
Hsv 1

The aim of this work was to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-HSV-1 activities of bio-compounds. Fifteen species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated 2 isolates of active bacteria. Ethyl acetate extracts from supernatants, indicating secreted compounds, showed an inhibition zone against MRSA at concentrations of 500-1000µg/ml. DNA sequence analysis showed that the strainKJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, the strain NP31-01 from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of P. rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1000 µg/ml. No anti-HSV-1 was observed. This is the first reported study describing research on anti-MRSA, cytotoxicity and anti-HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced and secreted byPseudoalteromonas rubra and Virgibacillus salarius, symbionts of Nudibranch, had potential anti-MRSA activity. Extracts from P. rubra showed cytotoxic effects on Vero cells, whereas extracts from V. salarius did not show cytotoxic effects. Three compounds were identified by LC/MS after purification from culture supernatant.

scholarly journals Complete genome sequence of Pseudoalteromonas rubra SCSIO 6842, harboring a putative conjugative plasmid pMBL6842

2016 ◽  
Vol 224 ◽  
pp. 66-67 ◽  
Author(s):  
Baiyuan Li ◽  
Pengxia Wang ◽  
Zhenshun Zeng ◽  
Xingsheng Cai ◽  
Guanghua Wang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Conjugative Plasmid ◽  
Pseudoalteromonas Rubra

scholarly journals Bioactivity of compounds secreted by symbiont bacteria of Nudibranchs from Indonesia

2019 ◽  
Author(s):  
Rhesi Kristiana ◽  
Gilles Bedoux ◽  
Gerard Pals ◽  
I Wayan Mudianta ◽  
Laure Taupin ◽  
...  
Keyword(s):  
Inhibition Zone ◽  
Vero Cells ◽  
Bacterial Symbionts ◽  
Cytotoxic Effects ◽  
Reported Study ◽  
Activity Screening ◽  
Screening Activity ◽  
Pseudoalteromonas Rubra ◽  
Ethyl Acetate Extracts ◽  
Hsv 1

The aim of this work was to isolate bacterial symbionts from nudibranchs and subsequently to determine anti-Methicillin resistant Staphylococcus aureus (MRSA), cytotoxicity and anti-HSV-1 activities of bio-compounds. Fifteen species of nudibranchs were collected from Karimunjawa and five species from Bali, respectively. A total of 245 bacteria isolates were obtained. The anti-MRSA activity screening activity indicated 2 isolates of active bacteria. Ethyl acetate extracts from supernatants, indicating secreted compounds, showed an inhibition zone against MRSA at concentrations of 500-1000µg/ml. DNA sequence analysis showed that the strainKJB-07 from Phyllidia coelestis was closely related to Pseudoalteromonas rubra, the strain NP31-01 from Phyllidia varicosa was closely related to Virgibacillus salarius. The extract of P. rubra was cytotoxic to Vero cells at a concentration of 75 µg/ml. The extract of V. salarius presented no cytotoxicity at concentrations of 5-1000 µg/ml. No anti-HSV-1 was observed. This is the first reported study describing research on anti-MRSA, cytotoxicity and anti-HSV-1 activity of bacterial symbionts from the viscera of nudibranch. Compounds produced and secreted byPseudoalteromonas rubra and Virgibacillus salarius, symbionts of Nudibranch, had potential anti-MRSA activity. Extracts from P. rubra showed cytotoxic effects on Vero cells, whereas extracts from V. salarius did not show cytotoxic effects. Three compounds were identified by LC/MS after purification from culture supernatant.

scholarly journals Genome Sequence of the Cycloprodigiosin-Producing Bacterial Strain Pseudoalteromonas rubra ATCC 29570T

2012 ◽  
Vol 194 (6) ◽  
pp. 1637-1638 ◽  
Author(s):  
B.-B. Xie ◽  
Y.-L. Shu ◽  
Q.-L. Qin ◽  
J.-C. Rong ◽  
X.-Y. Zhang ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Bacterial Strain ◽  
Pseudoalteromonas Rubra
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