Exemplar Abstract for Halomonas marina (Cobet et al. 1970) Dobson and Franzmann 1996, Cobetia marina (Cobet et al. 1970) Arahal et al. 2002 emend. Romanenko et al. 2013, Deleya marina (Cobet et al. 1970) Baumann et al. 1983 and Pseudomonas marina (Cobet et al. 1970) Baumann et al. 1972 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Sarah Wigley ◽  
George M Garrity
Keyword(s):  
Cobetia Marina ◽  
Deleya Marina

scholarly journals Cobetia crustatorum sp. nov., a novel slightly halophilic bacterium isolated from traditional fermented seafood in Korea

2010 ◽  
Vol 60 (3) ◽  
pp. 620-626 ◽  
Author(s):  
Min-Soo Kim ◽  
Seong Woon Roh ◽  
Jin-Woo Bae
Keyword(s):  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Nitrogen Sources ◽  
Optimal Growth ◽  
Halophilic Bacterium ◽  
Rrna Gene ◽  
Carbon And Nitrogen ◽  
Cobetia Marina ◽  
Slightly Halophilic Bacterium ◽  
Straight Rod

A slightly halophilic, Gram-stain-negative, straight-rod-shaped aerobe, strain JO1T, was isolated from jeotgal, a traditional Korean fermented seafood. Cells were observed singly or in pairs and had 2–5 peritrichous flagella. Optimal growth occurred at 25 °C, in 6.5 % (w/v) salts and at pH 5.0–6.0. Strain JO1T was oxidase-negative and catalase-positive. Cells did not reduce fumarate, nitrate or nitrite on respiration. Acid was produced from several carbohydrates and the strain utilized many sugars and amino acids as carbon and nitrogen sources. The main fatty acids were C12 : 0 3-OH, C16 : 0, C17 : 0 cyclo and summed feature 3 (C16 : 1 ω7c/iso-C15 : 0 2-OH). DNA–DNA hybridization experiments with strain JO1T and Cobetia marina DSM 4741T revealed 24 % relatedness, although high 16S rRNA gene sequence similarity (98.9 %) was observed between these strains. Based on phenotypic, genotypic and phylogenetic analyses, it is proposed that the isolate from jeotgal should be classified as a representative of a novel species, Cobetia crustatorum sp. nov., with strain JO1T (=KCTC 22486T=JCM 15644T) as the type strain.

Use of PCR–RFLP for genotyping 16S rRNA and characterizing bacteria cultured from halibut fry

2002 ◽  
Vol 48 (5) ◽  
pp. 379-386 ◽  
Author(s):  
Sigmund Jensen ◽  
Øivind Bergh ◽  
Øivind Enger ◽  
Brit Hjeltnes
Keyword(s):  
16S Rdna ◽  
Small Subunit ◽  
Atlantic Halibut ◽  
Normal Flora ◽  
Pathogen Invasion ◽  
Restriction Profiles ◽  
Pathogenic Vibrio ◽  
Efficient Detection ◽  
Pcr Rflp ◽  
Deleya Marina

Small subunit ribosomal genes were explored using PCR–RFLP to facilitate the characterization of bacteria cultured from reared fry of the Atlantic halibut (Hippoglossus hippoglossus). Concern has been expressed about pathogen invasion in larvae lacking a counteracting normal flora that may aid the immune system in producing robust noninfected individuals. In this study, pure cultured representatives of normal flora that were previously found to be antagonistic towards a pathogenic Vibrio sp. were subjected to a whole cell PCR protocol amplifying ~1500 bp of 16S rDNA. Amplified DNA was digested by AluI, BstUI, CfoI, and RsaI, to generate restriction profiles. Before the isolates were characterized, a survey was performed to test the discriminative efficiency of the RFLP. Efficient detection of polymorphism and the resolution of species and subspecies were achieved. Using the RFLP on 103 isolates generated as many as 22 genotypes. Based on the restriction profiles, a taxonomic tree incorporating 19 reference strains was constructed. Partial sequencing found this tree to be dominated by γ-Proteobacteria in clusters of Vibrio-, Pseudomonas-, and Alteromonas-affiliated species. Only nine isolates fell outside these genera, including the three isolates Shewanella alga, Deleya marina, and Marinomonas protea. These species have not previously been reported as halibut flora. The most frequently isolated genotype resembled Vibrio salmonicida.Key words: halibut, Vibrio, RFLP, 16S rDNA, phylogeny.

scholarly journals The marine bacteria Cobetia marina DSMZ 4741 synthesizes an unexpected K-antigen-like exopolysaccharide

2015 ◽  
Vol 124 ◽  
pp. 347-356 ◽  
Author(s):  
Florian Lelchat ◽  
Stéphane Cérantola ◽  
Christophe Brandily ◽  
Sylvia Colliec-Jouault ◽  
Anne-Claire Baudoux ◽  
...  
Keyword(s):  
Marine Bacteria ◽  
Cobetia Marina ◽  
K Antigen

scholarly journals Novel N-Acyl Amino Acid Produced by Deleya marina

1995 ◽  
Vol 44 (9) ◽  
pp. 635-639 ◽  
Author(s):  
Hisaaki YAGI ◽  
Gerardo CORZO ◽  
Toshihiro YOKOCHI ◽  
Yasushi KAMISAKA ◽  
Masakazu YAMAOKA ◽  
...  
Keyword(s):  
Amino Acid ◽  
Deleya Marina

Assessment of marine biofilm attachment and growth for antifouling surfaces under static and controlled hydrodynamic conditions

2011 ◽  
Vol 1356 ◽  
Author(s):  
Maria Salta ◽  
Julian A. Wharton ◽  
Paul Stoodley ◽  
Robert J.K. Wood ◽  
Keith R. Stokes
Keyword(s):  
Natural Product ◽  
Bacterial Attachment ◽  
Shear Stresses ◽  
Cell Integrity ◽  
Marine Biofilm ◽  
Cobetia Marina ◽  
Terrestrial Source ◽  
Isolated Compound ◽  
First Time

ABSTRACTThis investigation has assessed natural product antifouling performance of an isolated compound from a terrestrial source against marine biofilm forming bacteria, Cobetia marina and Marinobacter hydrocarbonoclasticus. Novel bioassay protocols using the hydrodynamic system and its well plate microfluidics capability were developed to test the in situ antifouling efficacy of the natural product against biofilm attachment under two shear stresses (0.07 and 0.3 Pa). The hydrodynamic results allowed for the first time the direct observation of the natural product influence on newly attached marine biofilms and the evolution of the antifouling affect with time. Biofilm attachment behaviour appeared to be markedly different in the presence of the natural product, illustrated by limited cluster and extracellular polymeric substance formation which suggests an interference of the bacterial attachment mechanisms. Ultimately, this is fundamental in developing greater understanding of the biofilm kinetics. These observations were confirmed using epifluoresence and confocal microscopy, with the additional corroborative data on bacterial cell integrity using the LIVE / DEAD nucleic acid kit.

Complete genome of Cobetia marina JCM 21022T and phylogenomic analysis of the family Halomonadaceae

2017 ◽  
Vol 36 (2) ◽  
pp. 528-536 ◽  
Author(s):  
Xianghai Tang ◽  
Kuipeng Xu ◽  
Xiaojuan Han ◽  
Zhaolan Mo ◽  
Yunxiang Mao
Keyword(s):  
Complete Genome ◽  
Phylogenomic Analysis ◽  
Cobetia Marina ◽  
The Family

Asymmetrie Reduction of Synthetic Ketones by Marine Microorganisms

1993 ◽  
Vol 48 (5-6) ◽  
pp. 451-456 ◽  
Author(s):  
Yoshimitsu Yamazaki ◽  
Akihiko Maruyama ◽  
Kuniaki Hosono ◽  
Takanori Higashihara ◽  
Harumi Kobayash
Keyword(s):  
Deep Sea ◽  
Sea Water ◽  
Micrococcus Luteus ◽  
Okinawa Trough ◽  
Optical Purity ◽  
Japan Trench ◽  
Marine Microorganisms ◽  
Terrestrial Environment ◽  
Deleya Marina ◽  
Optically Pure

Abstract Three strains of bacteria reducing (trifluoroacetyl)ferrocene (3) to optically pure (R)-2,2,2-trifluoro-l-hydroxyethylferrocene (4) and one bacterial strain reducing 3 to (S)-4 of moderate optical purity were isolated from sea-water collected at the coastal areas in Ibaraki prefecture of Japan. The former three strains were identified as Micrococcus lylae, Micrococcus luteus, and Deleya marina and the latter as Bacillus licheniformis. These strains also asymmetrically reduced some other synthetic ketones, e.g., 2,2,2-trifluoroacetophenone (7) and phenyl trimethylsilyl ketone (9). Further screening of microorganisms capable of reducing 3 was done with bacteria isolated from sea-water of the deep sea (Okinawa trough, Japan trench, and Mariana trough) and of the pelagic areas (Indian Ocean and South China Sea). Most of these marine strains preferentially reduced 3 to (R)-4 similar to the coastal strains, but the frequency of finding very highly enantioselective strains (i.e., those forming 4 of > 90% e.e.) was remark­ ably high in several sites of the deep sea and pelagic areas as compared with the coast and terrestrial environment.

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