Exemplar Abstract for Methylomonas methanica (ex Söhngen 1906) Whittenbury and Krieg 1984.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Methylomonas Methanica

scholarly journals An improved assay for bacterial methane mono-oxygenase: some properties of the enzyme from Methylomonas methanica

1975 ◽  
Vol 151 (2) ◽  
pp. 459-462 ◽  
Author(s):  
J Colby ◽  
H Dalton ◽  
R Whittenbury
Keyword(s):  
Metal Ions ◽  
Methane Oxidation ◽  
Metal Binding ◽  
Methylomonas Methanica

Extracts of Methylomonas methanica catalyse the O2-and NAD(P)H-dependent disappearance of bromomethane. The activity is unstable at 2 degrees C but is stable at --70 degrees C for several weeks. Bromomethane mono-oxygenase is particulate and is inhibited by metal-binding reagents, by compounds SKF 525A and Lilly 53325, by some metal ions and by acetylene. Evidence is presented that indicates that bromomethane mono-oxygenase is the enzyme responsible for methane oxidation in vivo.

scholarly journals The soluble methane mono-oxygenase of Methylococcus capsulatus (Bath). Its ability to oxygenate n-alkanes, n-alkenes, ethers, and alicyclic, aromatic and heterocyclic compounds

1977 ◽  
Vol 165 (2) ◽  
pp. 395-402 ◽  
Author(s):  
J Colby ◽  
D I Stirling ◽  
H Dalton
Keyword(s):  
Aromatic Compounds ◽  
Heterocyclic Compounds ◽  
Catalytic Properties ◽  
Methylococcus Capsulatus ◽  
Methylosinus Trichosporium ◽  
Styrene Epoxide ◽  
Cyclic Alkanes ◽  
Methylomonas Albus ◽  
Terminal Oxidation ◽  
Methylomonas Methanica

1. Methane mono-oxygenase of Methylococcus capsulatus (Bath) catalyses the oxidation of various substituted methane derivatives including methanol. 2. It is a very non-specific oxygenase and, in some of its catalytic properties, apparently resembles the analogous enzyme from Methylomonas methanica but differs from those found in Methylosinus trichosporium and Methylomonas albus. 3. CO is oxidized to CO2. 4. C1-C8 n-alkanes are hydroxylated, yielding mixtures of the corresponding 1- and 2-alcohols; no 3- or 4-alcohols are formed. 5. Terminal alkenes yield the corresponding 1,2-epoxides. cis- or trans-but-2-ene are each oxidized to a mixture of 2,3-epoxybutane and but-2-en-1-ol with retention of the cis or trans configuration in both products; 2-butanone is also formed from cis-but-2-ene only. 6. Dimethyl ether is oxidized. Diethyl ether undergoes sub-terminal oxidation, yielding ethanol and ethanal in equimolar amounts. 7. Methane mono-oxygenase also hydroxylates cyclic alkanes and aromatic compounds. However, styrene yields only styrene epoxide and pyridine yields only pyridine N-oxide. 8. Of those compounds tested, only NADPH can replace NADH as electron donor.

Exemplar Abstract for Methylomonas methanica (ex Söhngen 1906) Whittenbury and Krieg 1984.

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
Dorothea Taylor ◽  
George M Garrity
Keyword(s):  
Methylomonas Methanica

scholarly journals Draft Genome Sequences of Eight Obligate Methane Oxidizers Occupying Distinct Niches Based on Their Nitrogen Metabolism: TABLE 1 

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Kim Heylen ◽  
Paul De Vos ◽  
Bram Vekeman
Keyword(s):  
Nitrogen Metabolism ◽  
Nitrogen Assimilation ◽  
Draft Genome ◽  
Terrestrial Ecosystems ◽  
Genome Sequences ◽  
Aerobic Methanotrophs ◽  
Methylomonas Methanica

The genome sequences of Methylomonas methanica (NCIMB 11130 T , R-45363, and R-45371), Methylomonas koyamae (R-45378, R-45383, and R-49807), Methylomonas lenta (R-45370), and Methylosinus sp. (R-45379) were obtained. These aerobic methanotrophs were isolated from terrestrial ecosystems, and their distinct phenotypes related to nitrogen assimilation and dissimilation were previously reported.

Influence of kaolin on methane oxidation by Methylomonas methanica in gas phase bioreactors

Fuel ◽  
1992 ◽  
Vol 71 (7) ◽  
pp. 805-808 ◽  
Author(s):  
William A. Apel ◽  
Patrick R. Dugan ◽  
Michelle R. Wiebe
Keyword(s):  
Gas Phase ◽  
Methane Oxidation ◽  
Methylomonas Methanica

scholarly journals Polyclonal Antibodies Recognizing the AmoB Protein of Ammonia Oxidizers of the β-Subclass of the ClassProteobacteria

2001 ◽  
Vol 67 (1) ◽  
pp. 118-124 ◽  
Author(s):  
Claudia Pinck ◽  
Caroline Coeur ◽  
Patrick Potier ◽  
Eberhard Bock
Keyword(s):  
Polyclonal Antibodies ◽  
Immunoblot Analysis ◽  
Cell Protein ◽  
Ammonium Concentration ◽  
Methylococcus Capsulatus ◽  
Ammonia Oxidizers ◽  
A Subunit ◽  
Terminal Amino ◽  
Nitrosococcus Mobilis ◽  
Methylomonas Methanica

ABSTRACT A 41-kDa protein of Nitrosomonas eutropha was purified, and the N-terminal amino acid sequence was found to be nearly identical with the sequence of AmoB, a subunit of ammonia monooxygenase. This protein was used to develop polyclonal antibodies, which were highly specific for the detection of the four genera of ammonia oxidizers of the β-subclass of Proteobacteria(Nitrosomonas, including Nitrosococcus mobilis, which belongs phylogenetically to Nitrosomonas;Nitrosospira; Nitrosolobus; andNitrosovibrio). In contrast, the antibodies did not react with ammonia oxidizers affiliated with the γ-subclass ofProteobacteria (Nitrosococcus oceani andNitrosococcus halophilus). Moreover, methane oxidizers (Methylococcus capsulatus, Methylocystis parvus, and Methylomonas methanica) containing the related particulate methane monooxygenase were not detected. Quantitative immunoblot analysis revealed that total cell protein ofN. eutropha consisted of approximately 6% AmoB, when cells were grown using standard conditions (mineral medium containing 10 mM ammonium). This AmoB amount was shown to depend on the ammonium concentration in the medium. About 14% AmoB of total protein was found when N. eutropha was grown with 1 mM ammonium, whereas 4% AmoB was detected when 100 mM ammonium were used. In addition, the cellular amount of AmoB was influenced by the absence of the substrate. Cells starved for more than 2 months contained nearly twice as much AmoB as actively growing cells, although these cells possessed low ammonia-oxidizing activity. AmoB was always present and could even be detected in cells of Nitrosomonas after 1 year of ammonia starvation.

scholarly journals Complete Genome Sequence of the Aerobic Marine Methanotroph Methylomonas methanica MC09

2011 ◽  
Vol 193 (24) ◽  
pp. 7001-7002 ◽  
Author(s):  
R. Boden ◽  
M. Cunliffe ◽  
J. Scanlan ◽  
H. Moussard ◽  
K. D. Kits ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Methylomonas Methanica
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