scholarly journals Evaluation of Angiogenesis Using Micro-Computed Tomography in a Xenograft Mouse Model of Lung Cancer

Neoplasia ◽  
2009 ◽  
Vol 11 (1) ◽  
pp. 48-56 ◽  
Author(s):  
Rajkumar Savai ◽  
Alexander Claus Langheinrich ◽  
Ralph Theo Schermuly ◽  
Soni Savai Pullamsetti ◽  
Rio Dumitrascu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Lung Cancer ◽  
Mouse Model ◽  
Micro Computed Tomography ◽  
Xenograft Mouse Model

Quantification of neo-angiogenesis by micro-computed tomography in xenograft mouse model of lung cancer

2008 ◽  
Vol 6 (9) ◽  
pp. 21
Author(s):  
R. Savai ◽  
R.T. Schermuly ◽  
R. Dumitrascu ◽  
S.S. Pullamsetti ◽  
A.C. Langheinrich ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Lung Cancer ◽  
Mouse Model ◽  
Micro Computed Tomography ◽  
Xenograft Mouse Model

Double resin casting micro computed tomography (CT) reveals biliary and vascular pathology in tandem in a mouse model for alagille syndrome

2020 ◽  
Vol 73 ◽  
pp. S43
Author(s):  
Simona Hankeova ◽  
Jakub Salplachta ◽  
Tomas Zikmund ◽  
Michaela Kavkova ◽  
Noémi K.M. Van Hul ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Mouse Model ◽  
Alagille Syndrome ◽  
Vascular Pathology ◽  
Micro Computed Tomography

scholarly journals α-Hederin Inhibits the Growth of Lung Cancer A549 Cells in vitro and in vivo by Increasing SIRT6 Dependent Glycolysis

2020 ◽  
Author(s):  
cong fang ◽  
Yahui Liu ◽  
Lanying Chen ◽  
Yingying Luo ◽  
Yaru Cui ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Mouse Model ◽  
Protein Expression ◽  
Glucose Uptake ◽  
A549 Cells ◽  
Lactate Production ◽  
Tumor Xenograft ◽  
Xenograft Mouse Model ◽  
Atp Generation

Abstract Background: α-hederin an effective component of Pulsatilla chinensis (Bunge) Regel, Studies showed that α-hederin exert many pharmacological activities, However, the effect of α-hederin on metabolism is still unclear. This study aimed to illuminate the role of α-hederin in glucose metabolism in lung cancer cells and investigate the molecular mechanism of α-hederin. Methods: CCK8 and colony formation assays were employed to assess the anti-proliferative effects induced by α-hederin. Glucose uptake, ATP generation, and reduced lactate production were measured using kits, and an A549 tumor xenograft mouse model of lung cancer was used to assess the in vivo antitumor effect of α-hederin (5, 10 mg/kg). Glycolytic-related key enzymes hexokinase 2 (HK2), glucose transporters 1 (GLUT1), pyruvate kinase M2 (PKM2), lactate dehydrogenase A (LDHA), monocarboxylate transporter (MCT4), c-Myc, Hypoxia inducible factor-1α (HIF-1α) and Sirtuin 6 (SIRT6) protein expression were detected by western blotting and immunohistochemical staining and SIRT6 inhibitors was verified in A549 cells. Results: Our results showed that cell proliferation was significantly inhibited by α-hederin in a dose-dependent manner and that α-hederin inhibited glucose uptake and ATP generation and reduced lactate production. Furthermore, α-hederin remarkably inhibited HK2, GLUT1, PKM2, LDHA, MCT4, c-Myc, HIF-1α and activated SIRT6 protein expression. Using inhibitors, we proved that α-hederin inhibits glycolysis by activating SIRT6. Moreover, a tumor xenograft mouse model of lung cancer further confirmed that α-hederin inhibits lung cancer growth via inhibiting glycolysis in vivo. Conclusions: α-hederin inhibits the growth of non-small cell lung cancer A549 cells by inhibiting glycolysis. The mechanism of glycolysis inhibition includes α-hederin activating the expression of the glycolytic related protein SIRT6.

Longitudinal Follow-up of Cardiac Structure and Functional Changes in an Infarct Mouse Model Using Retrospectively Gated Micro-Computed Tomography

2008 ◽  
Vol 43 (7) ◽  
pp. 520-529 ◽  
Author(s):  
Sarah A. Detombe ◽  
Nancy L. Ford ◽  
Fuli Xiang ◽  
Xiangru Lu ◽  
Qingping Feng ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Mouse Model ◽  
Cardiac Structure ◽  
Micro Computed Tomography ◽  
Functional Changes

scholarly journals Quantification of Lung Fibrosis in IPF-Like Mouse Model and Pharmacological Response to Treatment by Micro-Computed Tomography

2020 ◽  
Vol 11 ◽  
Author(s):  
Francesca Ruscitti ◽  
Francesca Ravanetti ◽  
Valeria Bertani ◽  
Luisa Ragionieri ◽  
Laura Mecozzi ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Mouse Model ◽  
Lung Fibrosis ◽  
Response To Treatment ◽  
Micro Computed Tomography ◽  
Pharmacological Response
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