scholarly journals Replication Timing Aberrations and Aneuploidy in Peripheral Blood Lymphocytes of Breast Cancer Patients

Neoplasia ◽  
2010 ◽  
Vol 12 (8) ◽  
pp. 668-674 ◽  
Author(s):  
Helena Grinberg-Rashi ◽  
Samuel Cytron ◽  
Zully Gelman-Kohan ◽  
Talia Litmanovitch ◽  
Lydia Avivi
Keyword(s):  
Breast Cancer ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Replication Timing ◽  
Peripheral Blood Lymphocytes ◽  
Breast Cancer Patients ◽  
Blood Lymphocytes

Expression of critical genes used as prognostic biomarkers and DNA damage in lymphocytes from breast cancer patients

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 10641-10641 ◽  
Author(s):  
F. Franke ◽  
M. Agnoletto ◽  
J. Saffi ◽  
T. Guecheva
Keyword(s):  
Breast Cancer ◽  
Dna Damage ◽  
Dna Repair ◽  
Comet Assay ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Breast Cancer Patients ◽  
Dna Damage And Repair ◽  
Blood Lymphocytes

10641 Breast cancer is the most common malignancy among women and its rate of mortality is still high. The increase knowledge of breast cancer biology is heaving great impact on determining the clinical prognosis and response to treatment. Impaired DNA repair may elevate the risk of malignant transformation of breast cells due to the accumulation of spontaneous mutations in target genes and increasing susceptibility to exogenous carcinogens. The present study was designed to evaluate the relationship between DNA damage and expression of some critical genes including TP53, c-ERBB2, ER (Estrogen Receptor) and PR (Progesterone Receptor) in breast cancer. Blood samples were obtained from female patients with diagnosed breast cancer before chemotherapy as well as from healthy individuals, and were processed in 24 hours. To evaluate the role of DNA repair in breast cancer we determined the level of DNA damage and the capacity to remove DNA damage induced by hydrogen peroxide in the peripheral blood lymphocytes. For this purpose the alkaline version of the comet assay, which provides a sensitive tool to investigate DNA damage and repair, was applied. The level of basal DNA damage was higher in breast cancer patients compared to the control group. Considerable inter-individual variations of DNA damage and repair in breast cancer patients were observed both before and after the treatment. The correlation between DNA damage in peripheral blood and expression of p53, c-erbB-2, PR and ER was analyzed. This preliminary study indicates that the DNA damage accumulation, observed in peripheral blood lymphocytes of breast cancer patients in early stages, could be attributed to impaired DNA repair. Our results suggest that DNA damage, as evaluated by the comet assay, seems to be useful molecular biomarker for monitoring ongoing exposures to DNA damaging agents. Such a research on the mutagen sensitivity and efficacy of DNA repair could impact on the development of new diagnostic and screening strategies. Work Supported by FAPERGS and GENOTOX (UFRGS). No significant financial relationships to disclose.

DNA repair foci and late apoptosis/necrosis in peripheral blood lymphocytes of breast cancer patients undergoing radiotherapy

2015 ◽  
Vol 91 (12) ◽  
pp. 934-945 ◽  
Author(s):  
Eva Marková ◽  
Alexandra Somsedíková ◽  
Stanislav Vasilyev ◽  
Margita Pobijaková ◽  
Alica Lacková ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Dna Repair ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Breast Cancer Patients ◽  
Blood Lymphocytes ◽  
Late Apoptosis

scholarly journals FEATURES OF LYMPHOCYTES IN THE BLOOD IN PATIENTS WITH BASAL-LIKE BREAST CANCER SOUTH-EAST UKRAINE

2016 ◽  
Author(s):  
T. Yu. Pohorila ◽  
N. F. Shchurov ◽  
S. M. Pashchenko
Keyword(s):  
Breast Cancer ◽  
Peripheral Blood ◽  
Antitumor Immunity ◽  
Morphological Characteristics ◽  
Peripheral Blood Lymphocytes ◽  
Comprehensive Treatment ◽  
Breast Cancer Patients ◽  
Blood Lymphocytes ◽  
Before And After ◽  
Basal Like Breast Cancer

Lymphocytes are important in antitumor immunity. Therefore, the estimate of the magnitude of lymphocytes, could be used to determine the protective capabilities of the organism basal-like breast cancer patients. The purpose of the study: building a morphological features peripheral blood lymphocytes of patients with basal-like breast cancer South-East Ukraine, receiving comprehensive chemoradiotherapy. It was found that treatment BLT changes the structure of peripheral blood lymphocytes. After the comprehensive treatment of peripheral blood lymphocytes of patients significantly increased in size, they decreased nuclear-cytoplasmic ratio. For definitions immune status of patients basal-like breast cancer no small role played by morphological characteristics of lymphocytes in the peripheral blood before and after complex treatment.

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