scholarly journals The effect of press temperature on the total tocopherols, sterol, fatty acid, phenolic profile, in-vitro cytotoxicity assay, and anti-inflammatory activity

2021 ◽  
Author(s):  
Hakan BASDOGAN ◽  
Perihan Kübra AKMAN ◽  
Rusen Metin YILDIRIM ◽  
Osman SAGDIC ◽  
Umit GECGEL ◽  
...  
Keyword(s):  
Fatty Acid ◽  
In Vitro Cytotoxicity ◽  
Cytotoxicity Assay ◽  
Inflammatory Activity ◽  
Phenolic Profile ◽  
Press Temperature ◽  
Anti Inflammatory

scholarly journals Cordyceps militaris Fungus Extracts-Mediated Nanoemulsion for Improvement Antioxidant, Antimicrobial, and Anti-Inflammatory Activities

Molecules ◽  
2020 ◽  
Vol 25 (23) ◽  
pp. 5733
Author(s):  
Esrat Jahan Rupa ◽  
Jin Feng Li ◽  
Muhammad Huzaifa Arif ◽  
Han Yaxi ◽  
Aditi Mitra Puja ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Tween 80 ◽  
In Vitro Cytotoxicity ◽  
Cordyceps Militaris ◽  
Inflammatory Activity ◽  
Raw 264.7 Cells ◽  
No Production ◽  
Raw 264.7 ◽  
Anti Inflammatory

This study aimed to produce and optimize a Cordyceps militaris-based oil-in-water (O/W) nanoemulsion (NE) encapsulated in sea buckthorn oil (SBT) using an ultrasonication process. Herein, a nonionic surfactant (Tween 80) and chitosan cosurfactant were used as emulsifying agents. The Cordyceps nanoemulsion (COR-NE) was characterized using Fourier-transform infrared spectroscopy (FT-IR), dynamic light scattering (DLS), and field-emission transmission electron microscope (FE-TEM). The DLS analyses revealed that the NE droplets were 87.0 ± 2.1 nm in diameter, with a PDI value of 0.089 ± 0.023, and zeta potential of −26.20 ± 2. The small size, low PDI, and stable zeta potential highlighted the excellent stability of the NE. The NE was tested for stability under different temperature (4 °C, 25 °C, and 60 °C) and storage conditions for 3 months where 4 °C did not affect the stability. Finally, in vitro cytotoxicity and anti-inflammatory activity were assessed. The results suggested that the NE was not toxic to RAW 264.7 or HaCaT (human keratinocyte) cell lines at up to 100 µL/mL. Anti-inflammatory activity in liposaccharides (LPS)-induced RAW 264.7 cells was evident at 50 µg/mL and showed inhibition of NO production and downregulation of pro-inflammatory gene expression. Further, the NE exhibited good antioxidant (2.96 ± 0.10 mg/mL) activity and inhibited E. coli and S. aureus bacterial growth. Overall, the COR-NE had greater efficacy than the free extract and added significant value for future biomedical and cosmetics applications.

scholarly journals Fatty Acid Composition, Antioxidant, and in vitro Anti-inflammatory Activity of Five Cold-Pressed Prunus Seed Oils, and Their Anti-biofilm Effect Against Pathogenic Bacteria

2021 ◽  
Vol 8 ◽  
Author(s):  
Florinda Fratianni ◽  
Antonio d'Acierno ◽  
Maria Neve Ombra ◽  
Giuseppe Amato ◽  
Vincenzo De Feo ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Oleic Acid ◽  
Fatty Acid ◽  
Inflammatory Activity ◽  
Seed Oils ◽  
Black Cherry ◽  
Dpph Assay ◽  
Anti Inflammatory ◽  
Cold Pressed

Background/Aim: Sweet almond (Prunus amygdalus dulcis) oil is one of the most famous cold-pressed seed oils. However, other species of Prunus can provide oils with healthy properties. We analyzed the fatty acid (FA) composition, as well as the antioxidant, the in vitro anti-inflammatory properties, and the antibiofilm activity of five commercial vegetable cold-pressed seed oils of apricot, peach, plum, cherry, and black cherry.Methods: Gas Chromatography-Mass Spectrometry was performed for the analysis of FAs The antioxidant property of the oils was carried using different tests [2, 2-diphenyl-1-picrylhydrazyl (DPPH assay)], Ferric Reducing Antioxidant Power (FRAP), and the 2, 20 -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS·+). The denaturation assay performed on bovine serum albumin (BSA) was used to evaluate the in vitro anti-inflammatory activity. The anti-biofilm activity was assessed using five pathogenic strains, namely, Acinetobacter baumannii, Escherichia coli, Listeria monocytogenes, Pseudomonas aeruginosa, and Staphylococcus aureus, through the crystal violet test and the 3-(4,5-dimethylthiazol-2-yl)−2,5-diphenyltetrazolium bromide (MTT), used to evaluate the metabolism of the microbial cells present within the biofilm.Results: Oleic acid and linoleic acids were the most abundant FAs. Black cherry seed oil exhibited the best antioxidant activity, but in general, the amount of oil needed to inhibit the activity of 1 ml of DPPH assay at 50% did not exceed 10 μg. The extract concentration for the 50% inhibition of the denaturation of the protein (IC50) did not exceed 4.4 μg. Linoleic and stearic acids affected the antioxidant activity of the oils; oleic acid, linolenic, and palmitoleic acids exhibited beneficial effects in preserving the BSA denaturation, as shown by the correlation data. The oils were able to inhibit the biofilm formation of the pathogens (up to 71.40% of inhibition) as well as act against their mature biofilm, although with different strengths, with values up to 61.54%. Concurrently, they also acted on the pathogen metabolism.Conclusion: The oils represent a valuable source of some healthy FAs. They showed potential antioxidant and anti-inflammatory in vitro activity, in addition, their potential effect on the biofilm can offer important ideas for research and reflection on their use as functional foods and/or ingredients.

Synthesis, in vitro cytotoxicity and in vivo anti-inflammatory activity of long chain 3-amino-1,2-diols

1999 ◽  
Vol 9 (6) ◽  
pp. 821-826 ◽  
Author(s):  
JoséM. Padrón ◽  
Victor S. Martin ◽  
Dimitra Hadjipavlou-Litina ◽  
Caterina Noula ◽  
Violetta Constantinou-Kokotou ◽  
...  
Keyword(s):  
In Vitro Cytotoxicity ◽  
Inflammatory Activity ◽  
Anti Inflammatory ◽  
Long Chain

Phenolic profile and in vitro anti‐inflammatory activity of Mimosa scabrella Bentham honeydew honey in RAW 264.7 murine macrophages

2022 ◽  
Author(s):  
Bibiana Silva ◽  
Thiago Caon ◽  
Eduarda Talita Bramorski Mohr ◽  
Fabíola Carina Biluca ◽  
Luciano Valdomiro Gonzaga ◽  
...  
Keyword(s):  
Inflammatory Activity ◽  
Raw 264.7 ◽  
Phenolic Profile ◽  
Murine Macrophages ◽  
Anti Inflammatory

GC-MS Characterization, in vitro Antioxidant, Anti-inflammatory and Potential Antidermatophytic Activity of Citrullus lanatus Seed Oil

2021 ◽  
Vol 19 ◽  
Author(s):  
Ruhi Pathania ◽  
Prince Chawla ◽  
Abhishek Sharma ◽  
Ravinder Kaushik ◽  
Mohammed Azhar Khan
Keyword(s):  
Fatty Acid ◽  
Seed Oil ◽  
Citrullus Lanatus ◽  
Butylated Hydroxytoluene ◽  
Inflammatory Activity ◽  
Standard Drug ◽  
Fatty Acid Profiling ◽  
Anti Inflammatory ◽  
Antidermatophytic Activity

Background: Dermatophytes are well-known fungus having the capability to invade keratinized tissues of nails, skin, and hair, of humans and other animals, causing dermatophytosis. Citrullus lanatus (watermelon) seed is a rich source of vitamin E, essential fatty acids, and minerals, bearing various health benefits. Despite the presence of many useful components, the oil is not much explored. Accordingly, the study was planned to investigate the fatty acid profiling, in vitro antioxidant, anti-inflammatory, and potential antidermatophytic activity of Citrullus lanatus seed oil against dermatophytic strains. Methods: The C.lanatus oil was extracted from seeds, and fatty acid profiling was characterized by GC-MS. Further, the oil was subjected to DPPH scavenging activity, and the anti-inflammatory activity was evaluated from Bovine serum albumin assay. The antidermatophytic screening against Microsporum canis, Trichophyton rubrum, and Trichophyton mentagrophytes was carried out by agar well diffusion, broth microdilution assay, and growth kinetics assay. Results: The C. lanatus seed oil exhibited good antioxidant activity close to ascorbic acid, i.e., IC50- 52.22 µg/ml, 35.72 µg/ml, respectively, and the oil exhibited moderate anti-inflammatory activity (315.2 µg/ml) as that of the standard drug diclofenac sodium (174.3µg/ml). The fatty acid profiling indicates the presence of eight main compounds, out of which ç-tocopherol, butylated hydroxytoluene, and hexadecanoic acid are predominantly present. The C.lanatus seed oil showed promising activity against T. rubrum with a lower minimum inhibitory concentration value with respect to positive controls, Fluconazole and Ketoconazole. The oil also had a strong effect on time-dependent kinetic inhibition against T. rubrum. This was the first report of C.lanatus seed oil against any dermatophyte. Conclusion: In conclusion, the study supports the exploitation of C.lanatus seed oil and its main compounds as a source of natural antioxidants, anti-inflammatory, and as a novel agent against dermatophytes for curing superficial fungal infections in the near future.

Green Synthesis of Pectin-Gold-PLA-PEG-PLA Nanoconjugates: In Vitro Cytotoxicity and Anti-Inflammatory Activity

2017 ◽  
Vol 17 (7) ◽  
pp. 4549-4557 ◽  
Author(s):  
K Reena ◽  
M Prabakaran ◽  
B Leeba ◽  
M Gajendiran ◽  
S. Arul Antony
Keyword(s):  
Green Synthesis ◽  
In Vitro Cytotoxicity ◽  
Inflammatory Activity ◽  
Anti Inflammatory

Caffeine-loaded gold nanoparticles conjugated with PLA-PEG-PLA copolymer for in vitro cytotoxicity and anti-inflammatory activity

2017 ◽  
Vol 51 ◽  
pp. 113-121 ◽  
Author(s):  
Reena Kamalakannan ◽  
Gajendiran Mani ◽  
Prabakaran Muthusamy ◽  
Arul Antony Susaimanickam ◽  
Kyobum Kim
Keyword(s):  
Gold Nanoparticles ◽  
In Vitro Cytotoxicity ◽  
Inflammatory Activity ◽  
Anti Inflammatory
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