scholarly journals Analytical standards production for the analysis of pomegranate anthocyanins by HPLC

2014 ◽  
Vol 17 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Manuela Cristina Pessanha de Araújo Santiago ◽  
Ana Cristina Miranda Senna Gouvêa ◽  
Ronoel Luiz de Oliveira Godoy ◽  
Renata Galhardo Borguini ◽  
Sidney Pacheco ◽  
...  
Keyword(s):  
High Purity ◽  
High Performance ◽  
High Resolution Mass Spectrometry ◽  
Natural Antioxidants ◽  
Punica Granatum ◽  
Pomegranate Juice ◽  
High Purity Grade ◽  
Quantitative Analyses ◽  
Pomegranate Fruit ◽  
Analytical Standards

Pomegranate (Punica granatum L.) is a fruit with a long medicinal history, especially due to its phenolic compounds content, such as the anthocyanins, which are reported as one of the most important natural antioxidants. The analysis of the anthocyanins by high performance liquid chromatography (HPLC) can be considered as an important tool to evaluate the quality of pomegranate juice. For research laboratories the major challenge in using HPLC for quantitative analyses is the acquisition of high purity analytical standards, since these are expensive and in some cases not even commercially available. The aim of this study was to obtain analytical standards for the qualitative and quantitative analysis of the anthocyanins from pomegranate. Five vegetable matrices (pomegranate flower, jambolan, jabuticaba, blackberry and strawberry fruits) were used to isolate each of the six anthocyanins present in pomegranate fruit, using an analytical HPLC scale with non-destructive detection, it being possible to subsequently use them as analytical standards. Furthermore, their identities were confirmed by high resolution mass spectrometry. The proposed procedure showed that it is possible to obtain analytical standards of anthocyanins with a high purity grade (98.0 to 99.9%) from natural sources, which was proved to be an economic strategy for the production of standards by laboratories according to their research requirements.

scholarly journals Investigation of Anthocyanins Stability from Pomegranate Juice (Punica Granatum L. Cv Ermioni) under a Simulated Digestion Process

Medicines ◽  
2019 ◽  
Vol 6 (3) ◽  
pp. 90 ◽  
Author(s):  
Chrysavgi Gardeli ◽  
Kalliopi Varela ◽  
Eleni Krokida ◽  
Athanasios Mallouchos
Keyword(s):  
Antioxidant Activity ◽  
High Performance ◽  
Solid Phase ◽  
Punica Granatum ◽  
Pomegranate Juice ◽  
Human Organism ◽  
Total Phenolic ◽  
Parent Molecule ◽  
Digestion Process ◽  
Simulated Digestion

Background: Pomegranate gained a widespread popularity as a functional food due to the high content of bioactive components of the whole fruit, as well as its juice and extracts. There is a large amount of research that assigns them very important functions for the human organism. Methods: The anthocyanins (ACNs) of pomegranate juice (PJ) from the Ermioni variety are quantitatively identified and their stability under a simulated digestion process (SDP) is investigated. ACNs, as well as phenolic compounds, were isolated through solid phase extraction and determined using high-performance liquid chromatography in every stage of the SDP. Total phenolics, total monomeric ACNs, polymeric color and antioxidant activity were also determined in pomegranate juice and during the digestion process. Results: The predominant anthocyanin was Cy-3-glucoside followed by the corresponding 3,5-diglucoside, which accounted for 40.8% and 27.4% of the total ACN content, respectively. About 65% of the total monomeric ACN content remained intact by the end of the simulated digestion process. Conclusions: The PJ of the Ermioni variety seems to retain a large amount of the bioactive compounds after the SDP. The antioxidant activity and total phenolic content (TPC) remain almost stable during the SDP, suggesting that the products formed during ACN degradation maintain the antioxidant activity of the parent molecule.

scholarly journals Chromatographic analysis and antiproliferative potential of aqueous extracts of Punica granatum fruit peels using the Allium cepa test

2015 ◽  
Vol 51 (1) ◽  
pp. 241-248 ◽  
Author(s):  
Andrielle Wouters Kuhn ◽  
Marília Tedesco ◽  
Aline Augusti Boligon ◽  
Margareth Linde Athayde ◽  
Haywood Dail Laughinghouse IV ◽  
...  
Keyword(s):  
Allium Cepa ◽  
High Performance ◽  
Punica Granatum ◽  
Distilled Water ◽  
Fruit Peel ◽  
Subsequent Recovery ◽  
Allium Cepa L ◽  
Set Up ◽  
Pomegranate Fruit ◽  
Punica Granatum L

Punica granatum L., locally known as romanzeira, is native to Asia but found throughout Brazil. P. granatum is used for treating inflammatory, infectious and respiratory diseases. The aim of this study was to evaluate the chromatography and genotoxicity of an aqueous extract of P. granatum (pomegranate) fruit peel using the Allium cepa L. test. The experiment set-up entailed 7 treatments: T1-distilled water, T2-tea 5 g.L-1, T3-tea 10 g.L-1, T4-glyphosate at 9.6%, T5-glyphosate with subsequent recovery in distilled water, T6-glyphosate with subsequent recovery in tea 5 g.L-1 and T7-glyphosate with subsequent recovery in tea 10 g.L-1. The rootlets were collected and fixed in ethanol:acetic acid (3:1) for 24 hours, then stored in 70% ethanol under refrigeration. Analysis was performed using high performance liquid chromatography for the quantification of the extracted phenolic compounds. Gallic acid, catechin, caffeic acid, and rutin were abundant in the extracts of P. granatum. The extracts were found to exhibit antiproliferative potential but not antimutagenic or genotoxic activity.

scholarly journals Anthocyanins standards (cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside) isolation from freeze-dried açaí (Euterpe oleraceae Mart.) by HPLC

2012 ◽  
Vol 32 (1) ◽  
pp. 43-46 ◽  
Author(s):  
Ana Cristina Miranda Senna Gouvêa ◽  
Manuela Cristina Pessanha de Araujo ◽  
Daniel Filisberto Schulz ◽  
Sidney Pacheco ◽  
Ronoel Luis de Oliveira Godoy ◽  
...  
Keyword(s):  
High Performance ◽  
Molecular Ions ◽  
Published Data ◽  
Palm Fruit ◽  
High Purity Grade ◽  
Spectrometric Data ◽  
Standard Curve ◽  
Freeze Dried ◽  
Product Ions ◽  
Analytical Standards

Availability of analytical standards is a critical aspect in developing methods for quantitative analysis of anthocyanins. The anthocyanins cyanidin-3-O-glucoside and cyanidin-3-O-rutinoside were isolated from samples of freeze-dried açaí (Euterpe oleraceae Mart.), which is a round and purple well-known palm fruit in Brazil, and then used as standards. The isolation of the anthocyanins was performed by High Performance Liquid Chromatography (HPLC), using an adapted six-channel selection valve. The identification of anthocyanin pigments in açaí was based on mass spectrometric data for molecular ions and MS-MS product ions and on previous published data. After the collection procedure, standards with a high purity grade were obtained and an external standard curve of each anthocyanin was plotted.

scholarly journals Pharmacokinetic Drug Food Interaction Study of Nateglinide and Pomegranate Fruit Juice

2021 ◽  
Author(s):  
Shaikh Sameer D ◽  
Anasuya Patel ◽  
Patel Muftedar Ahmed ◽  
Syed Ayaz Ali
Keyword(s):  
Body Weight ◽  
Drug Interaction ◽  
Fruit Juice ◽  
Punica Granatum ◽  
Pomegranate Juice ◽  
Albino Rats ◽  
P Value ◽  
Food Interaction ◽  
Significant Drug Interaction ◽  
Pomegranate Fruit

Objective: Pomegranate juice is inhibitor of CYP450 enzyme system such as CYP2C9 and CYP3A4. The objective of the present study was to determine the consequence of pomegranate fruit juice on the pharmacokinetics of oral hypoglycemic drug nateglinide in rats. Materials and Methods: This is a laboratory study to investigate drug-food interaction effects of Punica granatum fruit juice (3 ml) and nateglinide (in doses 10 and 20 mg/kg body weight, p.o.) in live animals where the effects of food drug interaction on pharmacokinetics parameters such as Cmax and AUC in vehicle and nateglinide and pomegranate juice and nateglinide treated rats was undertaken. Four groups of Wistar albino rats comprising of (n=5) animals in each group were taken and treated with vehicle and nateglinide (in doses 10 and 20 mg/kg body weight, p.o.) pomegranate juice (3ml, p.o.) and nateglinide (in doses 10 and 20 mg/kg body weight, p.o.). Results: The rats which were administered with pomegranate juice + nateglinide (in doses 10 and 20 mg/kg body weight, p.o.) showed  raised Cmax to 2.85 fold and 2.21-fold respectively and an increase in AUC was found to be 1.34 fold and 1.47 fold respectively, when vehicle + nateglinide 10mg/kg and vehicle + 20mg/kg drug treated groups were compared. The results were compared at P-value< 0.01. Conclusion: A significant drug interaction was observed when nateglinide and pomegranate juice was administered indicating caution must be exercised when such food and drug is co-administered as the chance of more hypoglycemia may occur due to this potential drug-food interaction.

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