scholarly journals Sucralfate enemas reduce the oxidative tissue damage and preserves the contents of E-cadherin and ?-catenin in colonic mucosa without fecal stream

2021 ◽  
Vol 36 (10) ◽  
Author(s):  
Daniela Tiemi Sato ◽  
Fabio Guilherme Campos ◽  
Paulo Gustavo Kotze ◽  
Roberta Laís Santos Mendonça ◽  
Danilo Toshio Kanno ◽  
...  
Keyword(s):  
Tissue Damage ◽  
Colonic Mucosa ◽  
Fecal Stream ◽  
E Cadherin

E-Cadherin Expression in Colonic Mucosa with and Without Fecal Stream

2012 ◽  
Vol 26 (2) ◽  
pp. 72-79 ◽  
Author(s):  
Caled Jaoudat Kadri ◽  
José Aires Pereira ◽  
Camila Morais Gonçalves da Silva ◽  
Ronaldo Nonose ◽  
Enzo Fabrício do Nascimento ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Fecal Stream ◽  
E Cadherin

scholarly journals Reduction in oxidative stress levels in the colonic mucosa without fecal stream after the application of enemas containing aqueous Ilex paraguariensis extract

2011 ◽  
Vol 26 (4) ◽  
pp. 289-296 ◽  
Author(s):  
Fernando Lorenzetti da Cunha ◽  
Camila Morais Gonçalves da Silva ◽  
Marcos Gonçalves de Almeida ◽  
Thais Miguel do Monte Lameiro ◽  
Letícia Helena Souza Marques ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Aqueous Extract ◽  
Tissue Damage ◽  
Colonic Mucosa ◽  
Ilex Paraguariensis ◽  
Histopathological Analysis ◽  
Fecal Stream ◽  
Proximal Colostomy ◽  
Antioxidant Effects ◽  
Experimental Group

PURPOSE: To evaluate the antioxidant effects of enemas containing aqueous extract of Ilex paraguariensis, comparing segments with and without fecal stream and correlating the segments with the duration of intervention. METHODS: Twenty-six Wistar rats were subjected to a diversion of the fecal stream in the left colon by a proximal colostomy and distal mucosal fistula. The rats were distributed randomly into two experimental groups of 13 animals each based on the time of sacrifice after surgical procedure (two or four weeks). Each group was then divided into two experimental subgroups that received either second daily enemas containing 0.9% saline solution or aqueous extract of Ilex paraguariensis at 0.2g/100g. Colitis was diagnosed by histopathological analysis and the detection of oxidative tissue damage by measuring the levels of malondialdehyde. The Mann-Whitney test was used to compare the tissue levels of malondialdehyde between colon segments with and without fecal stream in each experimental group, and the Kruskal-Wallis test was used to verify the variance between the levels of oxidative stress according the duration of the irrigation; both tests determined significance at 5% (p<0.05). RESULTS: The levels of malondialdehyde in the animals subjected to intervention in the colon with saline with and without fecal stream after two and four weeks of irrigation were 0.05±0.006 and 0.06±0.006, and 0.05± 0.03 and 0.08 ±0.02, respectively. The malondialdehyde levels in the animals irrigated with Ilex paraguariensis with and without fecal stream after two and four weeks of irrigation were 0.010±0.002 and 0.02±0.004, and 0.03±0.007 and 0.04±0.01, respectively. After two and four weeks of intervention, the levels of malondialdehyde were lower in the animals irrigated with Ilex paraguariensis regardless of the time of irrigation (p=0.0001 and p=0.002, respectively). CONCLUSION: The daily rectal application of enemas containing aqueous extract of Ilex paraguariensis decreases oxidative tissue damage in the colon without fecal stream regardless of the time of irrigation.

5-Aminosalicylic Acid (5-ASA) Can Reduce Levels of Oxidative DNA Damage in Cells of Colonic Mucosa With and Without Fecal Stream

2010 ◽  
Vol 56 (4) ◽  
pp. 1037-1046 ◽  
Author(s):  
Caroline Caltabiano ◽  
Felipe Rodrigues Máximo ◽  
Ana Paula Pimentel Spadari ◽  
Daniel Duarte da Conceição Miranda ◽  
Marcia Milena Pivatto Serra ◽  
...  
Keyword(s):  
Dna Damage ◽  
Oxidative Dna Damage ◽  
Colonic Mucosa ◽  
Aminosalicylic Acid ◽  
Fecal Stream ◽  
In Cells

scholarly journals Influence of the application of enemas with oily extract of curcumin ( Curcuma longa ) on the tissue content of neutral and acidic mucins in the colonic mucosa without fecal stream

2017 ◽  
Vol 37 (4) ◽  
pp. 312-319
Author(s):  
Carlos Augusto Real Martinez ◽  
Vitor Piquera de Oliveira ◽  
Fabio Guilherme Campos ◽  
Danilo Toshio Kanno ◽  
Oscar Orlando Araya Fernandez ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Curcuma Longa ◽  
Fecal Stream ◽  
Tissue Content

scholarly journals Enemas with mesalazine increase the tissue contents of mucins in the colonic mucosa devoid of fecal stream

2019 ◽  
Vol 34 (4) ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
Fábio Guilherme Campos ◽  
Danilo Toshio Kanno ◽  
Eli Cristiano Meneses ◽  
Gabrielle Maira Matijascic ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Fecal Stream ◽  
Tissue Contents

Acetaldehyde disrupts tight junctions and adherens junctions in human colonic mucosa: protection by EGF and l-glutamine

2005 ◽  
Vol 289 (2) ◽  
pp. G367-G375 ◽  
Author(s):  
S. Basuroy ◽  
P. Sheth ◽  
C. M. Mansbach ◽  
R. K. Rao
Keyword(s):  
Tight Junction ◽  
Tyrosine Phosphorylation ◽  
Colonic Mucosa ◽  
Adherens Junction ◽  
Toxic Metabolite ◽  
Protein Tyrosine ◽  
Zonula Occludens ◽  
Increased Risk ◽  
Zonula Occludens 1 ◽  
E Cadherin

Acetaldehyde, a toxic metabolite of ethanol oxidation, is suggested to play a role in the increased risk for gastrointestinal cancers in alcoholics. In the present study, the effect of acetaldehyde on tyrosine phosphorylation, immmunofluorescence localization, and detergent-insoluble fractions of the tight junction and the adherens junction proteins was determined in the human colonic mucosa. The role of EGF and l-glutamine in prevention of acetaldehyde-induced effects was also evaluated. Acetaldehyde reduced the protein tyrosine phosphatase activity, thereby increasing the tyrosine phosphorylation of occludin, E-cadherin, and β-catenin. The levels of occludin, zonula occludens-1, E-cadherin, and β-catenin in detergent-insoluble fractions were reduced by acetaldehyde, while it increased their levels in detergent-soluble fractions. Pretreatment with EGF or l-glutamine prevented acetaldehyde-induced protein tyrosine phosphorylation, redistribution from intercellular junctions, and reduction in the levels of detergent-insoluble fractions of occludin, zonula occludens-1, E-cadherin, and β-catenin. These results demonstrate that acetaldehyde induces tyrosine phosphorylation and disrupts tight junction and adherens junction in human colonic mucosa, which can be prevented by EGF and glutamine.

scholarly journals Claudin-3 and occludin tissue content in the glands of colonic mucosa with and without a fecal stream

2015 ◽  
Vol 46 (2) ◽  
pp. 183-194 ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
Fabio Guilherme Caserta Maryssael de Campos ◽  
Viviel Rodrigo José de Carvalho ◽  
Caroline de Castro Ferreira ◽  
Murilo Rocha Rodrigues ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Fecal Stream ◽  
Tissue Content

scholarly journals Tissue content of sulfomucins and sialomucins in the colonic mucosa, without fecal stream, undergoing daily intervention with sucralfate

2015 ◽  
Vol 30 (5) ◽  
pp. 328-338 ◽  
Author(s):  
Christiny Emmanuelle Gabriel Bonassa ◽  
José Aires Pereira ◽  
Fábio Guilherme Caserta Maryssael de Campos ◽  
Murilo Rocha Rodrigues ◽  
Daniela Tiemi Sato ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Fecal Stream ◽  
Tissue Content

scholarly journals EVALUATION OF ENEMAS CONTAINING SUCRALFATE IN TISSUE CONTENT OF MUC-2 PROTEIN IN EXPERIMENTAL MODEL OF DIVERSION COLITIS

2017 ◽  
Vol 30 (2) ◽  
pp. 132-138 ◽  
Author(s):  
Oscar Orlando Araya FERNANDEZ ◽  
José Aires PEREIRA ◽  
Fábio Guilherme CAMPOS ◽  
Carolina Mardegan ARAYA ◽  
Gabriele Escocia MARINHO ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Experimental Models ◽  
Histopathological Study ◽  
Diversion Colitis ◽  
Fecal Stream ◽  
Inflammatory Score ◽  
Tissue Content ◽  
Proximal Colostomy ◽  
Computerized Morphometry ◽  
Mucous Fistula

ABSTRACT Background: The effects of topical application of sucralfate (SCF) on the tissue content of MUC-2 protein have not yet been evaluated in experimental models of diversion colitis. Aim: To measure the tissue content of MUC-2 protein in the colonic mucosa diverted from fecal stream submitted to the SCF intervention. Methods: Thirty-six rats underwent derivation of intestinal transit through proximal colostomy and distal mucous fistula. The animals were divided into three groups which were submitted application of enemas with saline, SCF 1 g/kg/day and SCF 2 g/kg/day. Each group was divided into two subgroups, according to euthanasia was done after two or four weeks. The colitis diagnosis was established by histopathological study and the inflammatory intensity was evaluated by previously validated scale. The MUC-2 protein was identified by immunohistochemistry and the tissue content was measured computerized morphometry). Results: The application of enemas with SCF in the concentration of 2 g/kg/day reduced inflammatory score of the segments that were diverted from fecal stream. The content of MUC-2 in diverted colon of the animals submitted to the intervention with SCF, independently of intervention period and the used concentration, was significantly greater than animals submitted to the application of enemas containing saline (p< 0.01). The content of MUC-2 after the intervention with SCF in the concentration of 2 g/kg/day was significantly higher when compared to the animals submitted to the application containing SCF at concentration of 1.0 g/kg/day (p<0.01). The tissue content of MUC-2 reached the highest values after intervention with SCF in the concentration of 2 g/kg/day for four weeks (p<0.01). Conclusion: The preventive application of enemas containing SCF reduces the inflammatory score and avoids the reduction of tissue content of MUC-2, suggesting that the substance is a valid therapeutic strategy to preserve the mucus layer that covers the intestinal epithelium.

scholarly journals Claudin-3 and occludin content in the glands of colonic mucosa devoid from fecal stream submitted to topical intervention with oil extract of Curcuma longa

2017 ◽  
Vol 32 (1) ◽  
pp. 65-73 ◽  
Author(s):  
Carlos Augusto Real Martinez ◽  
◽  
Caled Jaoudat Kadri ◽  
Danilo Toshio Kanno ◽  
Antônio José Tibúrcio Alves Júnior ◽  
...  
Keyword(s):  
Colonic Mucosa ◽  
Curcuma Longa ◽  
Fecal Stream ◽  
Oil Extract
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