Unusual Role of Tryptophan residues in Structural and Functional properties of Mimi Virus TyrRS (TyrRSapm)

2019 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Functional Properties ◽  
Structural And Functional Properties ◽  
Tryptophan Residues

scholarly journals The role of Tb-doping on the structural and functional properties of Bi4−xTbxTi3O12 ferroelectric phases with the Aurivillius type structure

2016 ◽  
Vol 28 (6) ◽  
pp. 4914-4924 ◽  
Author(s):  
Tapati Sarkar ◽  
Sergey A. Ivanov ◽  
Elena A. Fortalnova ◽  
Ekaterina D. Politova ◽  
Marina G. Safronenko ◽  
...  
Keyword(s):  
Functional Properties ◽  
Type Structure ◽  
Structural And Functional Properties

The key role of E418 carboxyl group in the formation of Nt.BspD6I nickase active site: Structural and functional properties of Nt.BspD6I E418A mutant

2020 ◽  
Vol 210 (3) ◽  
pp. 107508
Author(s):  
Rimma I. Artyukh ◽  
Galina S. Kachalova ◽  
Alfiya K. Yunusova ◽  
Bulat F. Fatkhullin ◽  
Boris P. Atanasov ◽  
...  
Keyword(s):  
Functional Properties ◽  
Active Site ◽  
Carboxyl Group ◽  
Structural And Functional Properties

scholarly journals Structural and functional properties of CiNTH, an endonuclease III homologue of the ascidian Ciona intestinalis: critical role of N-terminal region

2012 ◽  
Vol 87 (2) ◽  
pp. 115-124 ◽  
Author(s):  
Seiji Kato ◽  
Kazunari Hashiguchi ◽  
Kento Igarashi ◽  
Takahito Moriwaki ◽  
Shin-Ichiro Yonekura ◽  
...  
Keyword(s):  
Functional Properties ◽  
Critical Role ◽  
Ciona Intestinalis ◽  
Terminal Region ◽  
Endonuclease Iii ◽  
Structural And Functional Properties

scholarly journals Structural and Functional Peculiarities of Cytoplasmic Tropomyosin Isoforms, the Products of TPM1 and TPM4 Genes

2021 ◽  
Vol 22 (10) ◽  
pp. 5141
Author(s):  
Marina Marchenko ◽  
Victoria Nefedova ◽  
Natalia Artemova ◽  
Sergey Kleymenov ◽  
Dmitrii Levitsky ◽  
...  
Keyword(s):  
Functional Properties ◽  
Coiled Coil ◽  
Major Protein ◽  
Protein Dimers ◽  
Protein Partners ◽  
Sequence Variations ◽  
Structural And Functional Properties ◽  
Alternatively Spliced ◽  
Alternatively Spliced Exons

Tropomyosin (Tpm) is one of the major protein partners of actin. Tpm molecules are α-helical coiled-coil protein dimers forming a continuous head-to-tail polymer along the actin filament. Human cells produce a large number of Tpm isoforms that are thought to play a significant role in determining actin cytoskeletal functions. Even though the role of these Tpm isoforms in different non-muscle cells is more or less studied in many laboratories, little is known about their structural and functional properties. In the present work, we have applied various methods to investigate the properties of five cytoplasmic Tpm isoforms (Tpm1.5, Tpm 1.6, Tpm1.7, Tpm1.12, and Tpm 4.2), which are the products of two different genes, TPM1 and TPM4, and also significantly differ by alternatively spliced exons: N-terminal exons 1a2b or 1b, internal exons 6a or 6b, and C-terminal exons 9a, 9c or 9d. Our results demonstrate that structural and functional properties of these Tpm isoforms are quite different depending on sequence variations in alternatively spliced regions of their molecules. The revealed differences can be important in further studies to explain why various Tpm isoforms interact uniquely with actin filaments, thus playing an important role in the organization and dynamics of the cytoskeleton.

scholarly journals Microglia mediate synaptic plasticity induced by 10 Hz repetitive magnetic stimulation

2021 ◽  
Author(s):  
Amelie Eichler ◽  
Dimitrios Kleidonas ◽  
Zsolt Turi ◽  
Matthias Kirsch ◽  
Dietmar Pfeifer ◽  
...  
Keyword(s):  
Synaptic Plasticity ◽  
Functional Properties ◽  
Magnetic Stimulation ◽  
Pyramidal Neurons ◽  
Therapeutic Effects ◽  
Tissue Cultures ◽  
Ca1 Pyramidal Neurons ◽  
Structural And Functional Properties ◽  
Repetitive Magnetic Stimulation

Repetitive transcranial magnetic stimulation (rTMS) is a non-invasive brain stimulation technique that is widely used in clinical practice for therapeutic purposes. Nevertheless, the mechanisms that mediate its therapeutic effects remain poorly understood. Recent work implicates that microglia, the resident immune cells of the central nervous system, have a defined role in the regulation of physiological brain function, e.g. the expression of synaptic plasticity. Despite this observation, no evidence exists for a role of microglia in excitatory synaptic plasticity induced by rTMS. Here, we used repetitive magnetic stimulation of organotypic entorhino-hippocampal tissue cultures to test for the role of microglia in synaptic plasticity induced by 10 Hz repetitive magnetic stimulation (rMS). For this purpose, we performed PLX3397 (Pexidartinib) treatment to deplete microglia from tissue culture preparations. Using whole-cell patch-clamp recordings, live-cell microscopy, immunohistochemistry and transcriptome analysis, we assessed structural and functional properties of both CA1 pyramidal neurons and microglia to correlate the microglia phenotype to synaptic plasticity. PLX3397 treatment over 18 days reliably depletes microglia in tissue cultures, without affecting structural and functional properties of CA1 pyramidal neurons. Microglia-depleted cultures display defects in the ability of CA1 pyramidal neurons to express plasticity of excitatory synapses upon rMS. Notably, rMS induces a moderate release of proinflammatory and plasticity-promoting factors, while microglial morphology stays unaltered. We conclude that microglia play a crucial role in rMS-induced excitatory synaptic plasticity.

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