Expression of Somatic Embryogenesis Receptor Kinase (SERK) gene and its regulation under the influence of exogenous additives during in vitro somantic embryo development in medicinal plants

2020 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Somatic Embryogenesis ◽  
Medicinal Plants ◽  
Embryo Development ◽  
Receptor Kinase ◽  
Somatic Embryogenesis Receptor Kinase

scholarly journals Agrobacterium-mediated transformation of the wild orchid Cattleya maxima Lindl

2018 ◽  
Vol 23 (1) ◽  
pp. 89
Author(s):  
Augusta Yadira Cueva-Agila ◽  
Rino Cella
Keyword(s):  
Somatic Embryogenesis ◽  
Somatic Embryos ◽  
Attractive Alternative ◽  
Gene Encoding ◽  
Anatomical Structures ◽  
Large Numbers ◽  
Hygromycin Resistance Gene ◽  
Growing Conditions ◽  
Somatic Embryogenesis Receptor Kinase

Protocorms are unique anatomical structures; they are akin to rhizoids and are formed by young orchid seedlings under physiological conditions. Explanted orchid tissues produce similar structures called protocorm-like bodies (PLBs) when exposed to appropriate <em>in vitro</em> growing conditions. Both the propagative nature of PLBs and the easiness by which they can be generated, make these structures an attractive alternative to seed-mediated production for growing large numbers of plants. To increase somatic embryogenesis and optimize the procedure, PLBs of <em>Cattleya maxima </em>were transformed<em> </em>using the <em>Agrobacterium tumefaciens </em>method. The T-DNA carried a Hygromycin-resistance gene, a visible marker (GFP5-GUSA) and a rice gene encoding the Somatic Embryogenesis Receptor Kinase, deemed to be important for somatic embryogenesis. Treated PLBs generated somatic embryos developing Hygromycin-resistant plantlets. The insertion of T-DNA was confirmed by PCR, and GFP expression was observed using a fluorescent stereomicroscope. Transformed <em>Cattleya maxima</em> PLBs were more efficient in forming somatic embryos (60-80%) than untransformed controls (45-57%), and this contrast was maximized in hormone-free, Murashige and Skoog (MS) medium (80% of the transformed plants compared to 57% of the untransformed ones). This finding supports the notion that SERK<em> </em>plays an important role in Orchid embryogenesis.

scholarly journals GENE EXPRESSION DURING INDIRECT SOMATIC EMBRYOGENESIS OF PLANTS

2004 ◽  
Vol 42 (2) ◽  
Author(s):  
Tammy Estabrooks ◽  
Zhongmin Dong
Keyword(s):  
Gene Expression ◽  
Somatic Embryogenesis ◽  
Embryo Development ◽  
Current Literature ◽  
Molecular Mechanisms ◽  
Indirect Somatic Embryogenesis ◽  
Experimental Tool ◽  
Plant Embryo ◽  
Plant Embryo Development

Somatic embryogenesis is the process by which somatic cells are induced into an embryogenic state, followed by differentiation into embryos. Somatic embryogenesis, in addition to being a method of propagation, can serve as an experimental tool for research into plant embryo development. This is a review of the current literature on in vitro plant somatic embryogenesis and the molecular advances made to identify genes expressed during the various stages of this process. Some factors hindering the elucidation of the molecular mechanisms underlying somatic embryogenesis are discussed.L’embryogenèse somatique est le processus par lequel les cellules somatiques passent à l’état embryogène et se différencient en embryons. En plus de constituer une méthode de propagation, elle peut servir d’outil expérimental de recherche pour développer des embryons de plantes. Le présent document est une revue de la documentation sur l’embryogenèse somatique végétale in vitro et sur les progrès réalisés à l’échelle moléculaire pour identifier les gènes exprimés au cours des divers stades du processus. On examine aussi certains facteurs qui rendent difficile l’élucidation des mécanismes moléculaires de l’embryogenèse somatique.

Expression of the Daucus carota somatic embryogenesis receptor kinase (DcSERK) protein in insect cells

Biochimie ◽  
2001 ◽  
Vol 83 (5) ◽  
pp. 415-421 ◽  
Author(s):  
Khalid Shah ◽  
Ed D.L. Schmidt ◽  
Just M. Vlak ◽  
Sacco C. de Vries
Keyword(s):  
Somatic Embryogenesis ◽  
Daucus Carota ◽  
Insect Cells ◽  
Receptor Kinase ◽  
Somatic Embryogenesis Receptor Kinase

Subcellular Localization and Oligomerization of the Arabidopsis thaliana Somatic Embryogenesis Receptor Kinase 1 Protein

2001 ◽  
Vol 309 (3) ◽  
pp. 641-655 ◽  
Author(s):  
Khalid Shah ◽  
Theodorus W.J. Gadella Jr ◽  
Harrie van Erp ◽  
Valérie Hecht ◽  
Sacco C. de Vries
Keyword(s):  
Arabidopsis Thaliana ◽  
Somatic Embryogenesis ◽  
Subcellular Localization ◽  
Receptor Kinase ◽  
Somatic Embryogenesis Receptor Kinase

scholarly journals The Arabidopsis Somatic Embryogenesis Receptor Kinase 1 Gene Is Expressed in Developing Ovules and Embryos and Enhances Embryogenic Competence in Culture

2001 ◽  
Vol 127 (3) ◽  
pp. 803-816 ◽  
Author(s):  
Valérie Hecht ◽  
Jean-Philippe Vielle-Calzada ◽  
Marijke V. Hartog ◽  
Ed D.L. Schmidt ◽  
Kim Boutilier ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Receptor Kinase ◽  
Embryogenic Competence ◽  
Somatic Embryogenesis Receptor Kinase

Improvement of soybean somatic embryo development and maturation by abscisic acid treatment

2000 ◽  
Vol 80 (2) ◽  
pp. 271-276 ◽  
Author(s):  
Lining Tian ◽  
Daniel C. W. Brown
Keyword(s):  
Somatic Embryogenesis ◽  
Abscisic Acid ◽  
Embryo Development ◽  
Development Stage ◽  
Normal Embryo ◽  
Maturation Stage ◽  
Globular Stage ◽  
Solid Media ◽  
Stage Embryo

Recovery of tissue culture-derived plants through somatic embryogenesis is a useful system for genetic engineering of soybean. The effect of abscisic acid (ABA) on soybean somatic embryogenesis, development, and maturation was investigated. ABA at 1, 10, 50, 100, and 500 µM were applied at different stages of embryo development; namely, at the globular stage in suspension culture, at the development stage and at the maturation stage on solid media. ABA promoted embryo growth and development when applied at the globular stage. Embryo size, after 15 d and after 1 mo on development medium, was significantly greater than that without exposure to ABA. ABA promoted normal embryo morphogenesis and 62% more normal embryos developed when embryos were treated with ABA at the globular stage. ABA treated-embryos showed an increased tolerance to partial desiccation (from 24% to 78%) and exhibited an increased germination capability relative to non-ABA-treated controls (54% versus 8%). Somatic embryos appeared to undergo a decreasing sensitivity to ABA during maturation. ABA did not show an effect when applied during embryo development and maturation stages. A protocol for more normal embryo formation and improved embryo germination is reported. Key words: Glycine max, somatic embryogenesis, in vitro culture

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