scholarly journals Exogenous 5-Aminolevulenic Acid Promotes Antioxidative Defence System, Photosynthesis and Growth in Soybean against Cold Stress

2015 ◽  
Vol 7 (4) ◽  
pp. 486-494 ◽  
Author(s):  
Elahe MANAFI ◽  
Seyed Ali Mohammad MODARRES SANAVY ◽  
Majid AGHAALIKHANI ◽  
Aria DOLATABADIAN
Keyword(s):  
Plant Growth ◽  
Cold Stress ◽  
Water Content ◽  
Electrolyte Leakage ◽  
Relative Water Content ◽  
Seed Priming ◽  
Content Increase ◽  
Stomatal Conductivity ◽  
Relative Water ◽  
Soybean Plants

In the present study, the possibility of enhancing cold stress tolerance of young soybean plants (Glycine max [L.] Merr) by exogenous application of 5-aminolevulinic acid (ALA) was investigated. ALA was applied at various concentrations (0, 0.3, 0.6 and 0.9 mM) by seed priming and foliar application method. After ALA treatment, the plants were subjected to cold stress at 10 ± 0.5 °C for 72 h. Cold stress significantly decreased plant growth, relative water content, chlorophyll, photosynthesis and stomatal conductivity, while it increased electrolyte leakage and proline accumulation. ALA at low concentrations (0.3 mM) protected plants against cold stress, enhancing plant height, shoot fresh and dry weight, chlorophyll content, photosynthesis, stomatal conductivity as well as relative water content. Increase of electrolyte leakage was also prevented by 0.6 mM ALA. ALA also enhanced superoxide dismutase and catalase activities at 0.6 mM concentration especially under cold stress conditions. Proline increased with increasing in ALA concentration under both temperature conditions. In most cases, application of ALA by spraying method was better than seed priming method. Results showed that ALA, which is considered as an endogenous plant growth regulator, can be used effectively to protect soybean plants from the damaging effects of cold stress, by enhancing the activity of antioxidative enzymes, protecting cell membrane against reactive oxygen species and finally by promoting chlorophyll synthesis, leading to more intense photosynthesis and more carbon fixation, without any adverse effect on the plant growth.

scholarly journals Effect of Salt-Tolerant Bacterial Inoculations on Rice Seedlings Differing in Salt-Tolerance under Saline Soil Conditions

Agronomy ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 1030 ◽  
Author(s):  
Rakiba Shultana ◽  
Ali Tan Kee Zuan ◽  
Mohd Rafii Yusop ◽  
Halimi Mohd Saud ◽  
Arolu Fatai Ayanda
Keyword(s):  
Salt Tolerance ◽  
Plant Growth ◽  
Water Content ◽  
Electrolyte Leakage ◽  
Relative Water Content ◽  
Rice Variety ◽  
Salt Tolerant ◽  
Relative Water ◽  
Plant Growth Promoting ◽  
Growth Promoting

Salt-tolerant plant growth-promoting rhizobacteria (PGPR) could be an alternative to alleviate salinity problems in rice plants grown in the coastal areas. This study was conducted to isolate and characterize salt-tolerant PGPR and observe their effects on the physiological and biochemical properties of rice plants grown under non-saline and saline glasshouse conditions. Three strains were selected based on their salt-tolerance and plant growth-promoting properties under in vitro saline conditions. These strains were identified as Bacillus tequilensis (UPMRB9), Bacillus aryabhattai (UPMRE6), and Providencia stuartii (UPMRG1) using a 16S rRNA technique. The selected strains were inoculated to three different rice varieties, namely BRRI dhan67 (salt-tolerant), Putra-1 (moderate salt-tolerant), and MR297 (salt-susceptible) under glasshouse conditions. Results showed that the MR297 rice variety inoculated with UPMRB9 produced the highest total chlorophyll content, with an increment of 28%, and lowest electrolyte leakage of 92%. The Putra-1 rice variety also showed a 156% total dry matter increase with the inoculation of this bacterial strain. The highest increase of relative water content and reduction of Na/K ratio were found upon inoculation of UPMRE6 and UPMRB9, respectively. The biggest significant effects of these bacterial inoculations were on relative water content, electrolyte leakage, and the Na/K ratio of the BRRI dhan67 rice variety under saline conditions, suggesting a synergistic effect on the mechanisms of plant salt-tolerance. This study has shown that the application of locally-isolated salt-tolerant PGPR strains could be an effective long-term and sustainable solution for rice cultivation in the coastal areas, which are affected by global climate change.

scholarly journals Effect of cold stress on boro rice seedlings

2017 ◽  
Vol 9 (2) ◽  
pp. 1036-1041 ◽  
Author(s):  
Priyanka Kumari ◽  
H. K. Jaiswal
Keyword(s):  
Cold Stress ◽  
Water Content ◽  
Chlorophyll Content ◽  
Relative Water Content ◽  
Stability Index ◽  
Membrane Stability ◽  
Membrane Stability Index ◽  
Relative Water ◽  
Cold Tolerant ◽  
Boro Rice

Cold stress at seedling stage is a major constraint in boro rice production. Nine boro rice lines were crossed in diallel fashion excluding reciprocals to obtain 36 crosses. All the 36 crosses along with parents were grown in nursery in three seasons (boro-2014, kharif-2015 and boro-2015). Performance of seedlings for survival per cent, chlorophyll content, relative water content, membrane stability index was recorded just before transplanting in all the three seasons. Scoring for cold tolerance was done in both boro seasons. Gautam showed highest survival rate over three seasons. Among crosses, IR 64 x Krishna Hamsa showed highest survival (84%) in boro-2014, MTU 1010 x Jaya (86.33%) in boro-2015 and MTU 1010 x Krishna Hamsa (95.67%) in kharif-2015. Jaya x Krishna Hamsa was most cold tolerant cross over both boro seasons. Significant positive correlation was observed among survival per cent, chlorophyll content, relative water content and membrane stability index over seasons.

scholarly journals Biochemical metabolism of two cultivars of cowpea treated with 24-Epibrassinolide and subjected to saline stress

2019 ◽  
Vol 13 ((03) 2019) ◽  
pp. 444-451
Author(s):  
Kerolém Prícila Sousa Cardoso ◽  
Susana Silva Conceição ◽  
Ana Ecídia de Araújo Brito ◽  
Jéssica Taynara da Silva Martins ◽  
Liliane Corrêa Machado ◽  
...  
Keyword(s):  
Amino Acids ◽  
Nitrate Reductase ◽  
Water Content ◽  
Glycine Betaine ◽  
Electrolyte Leakage ◽  
Relative Water Content ◽  
Reductase Activity ◽  
Soluble Proteins ◽  
Saline Stress ◽  
Relative Water

We aimed to evaluate the changes in biochemical metabolism generated by salt stress and to investigate the effect of brassinosteroids in mitigating of this stress on two cultivars of Vigna unguiculata L. We used a completely randomized experimental design in a 2 x 3 x 3 factorial scheme, using two cultivars of cowpea (BRS Guariba and BR3 Tracuateua - moderately tolerant and sensitive to salinity, respectively), three concentrations of brassinosteroids (0, 0.2 and 0.4 μM Br) and three concentrations of NaCl (0 , 50 and 100 mM NaCl), with four replicates. The following evaluations were carried out: relative water content, electrolyte leakage, nitrate levels, nitrate reductase activity, free ammonium, total soluble amino acids, soluble proteins, glycine betaine and proline. The results showed that salinity at 100 mM affected the nitrate reductase enzyme activity, the relative water content, total soluble amino acids and soluble proteins for cultivars BR3 Tracuateua, and BRS Guariba, but the 24-epibrassinolid attenuated the effects of salinity for these variables. The concentration of 0.2 μM of Br increased 55% and 20% in proline and glycine betaine contents, respectively, in both of cultivars. The plants under stress saline and 0.2 mM of 24-epibrassinolid, presented 42% and 58% reductions in electrolyte leakage of BR3 Tracuateua and BRS Guariba cultivars, respectively. The concentrations of ammonium were slightly varied. Therefore, the application of 0.2 μM of 24-epibrassinolid caused a greater acclimatization of the cultivars, being the BR3 Tracuateua (sensitive to salt) cultivar more expressive in most treatments.

Increased drought resistance of black locust seedlings via pretreatment of seeds with paclobutrazol

1993 ◽  
Vol 23 (12) ◽  
pp. 2548-2551 ◽  
Author(s):  
Shen Hui-Juan ◽  
Zeng Bin
Keyword(s):  
Osmotic Stress ◽  
Water Content ◽  
Drought Resistance ◽  
Electrolyte Leakage ◽  
Relative Water Content ◽  
Black Locust ◽  
Cellular Stress ◽  
Relative Water

The drought resistance of black locust (Robiniapseudoacacia L.) seedlings was increased by pretreatment of seeds with 250 ppm paclobutrazol. Treated seeds produced seedlings with thick roots and higher root/shoot ratios. During osmotic stress, treated plants displayed greater relative water content, lower electrolyte leakage, and fewer symptoms of wilt when compared with untreated black locust seedlings. Paclobutrazol-treated seedlings accumulated more proline than untreated seedlings in response to drought. However, osmotically induced accumulation of putrescine, a possible marker of cellular stress, was reduced in the treated plants.

scholarly journals Changes in plant growth, leaf relative water content and physiological traits in response to salt stress in peanut (Arachis hypogaea L.) varieties

2021 ◽  
Vol 49 (1) ◽  
pp. 12049
Author(s):  
Tekam L. MEGUEKAM ◽  
Dany P. MOUALEU ◽  
Victor D. TAFFOUO ◽  
Hartmut STÜTZEL
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Water Content ◽  
Arachis Hypogaea ◽  
Relative Water Content ◽  
Physiological Traits ◽  
Salt Tolerant ◽  
Arachis Hypogaea L ◽  
Relative Water ◽  
Leaf Relative Water Content

Salinity is the main environmental factor accountable for decreasing crop productivity worldwide. The effects of NaCl salinity on plant growth (leaf relative water content (RWC), leaf dry weight (LDW), shoot length (SL), number of leaves (NL), number of branches (NB) and total leaf area (TLA) and physiological characteristics (stomatal conductance (gs), transpiration rate (TR), net photosynthetic (Pn), yield of photosystem II (ΦPsII) and the intercellular CO2 concentration (CO2int) in peanut (Arachis hypogaea L.) varieties (‘Vanda’, ‘P244601’ and ‘Pl184948’, widely used in Cameroon, Tanzania and Ghana, respectively, were investigated under hydroponic condition. Plants were subjected to four levels of NaCl (0, 40, 80 and 120 mM) at early seedling growth stage of plant development. Application of NaCl treatment led to a significant decrease in LDW, SL, NL, TLA, Pn, gs, TR and CO2int concentration of ‘Vanda’ and ‘P244601’ compared to untreated plants while the plant growth inhibition was notably noted at 120 mM NaCl in ‘P1184948’ for LDW, SL and NB. The highest depressive effect was detected in gs of salt-sensitive ‘Vanda’ while the lowest were recorded in gs of salt-tolerant ‘P1184948’ at high salinity level. Enhanced NaCl concentrations led to a significant increase in ΦPSII of ‘P1184948’ compared to ‘Vanda’, ‘P244601’ and untreated plants. Leaf CHL content was significantly increased in moderately-tolerant ‘‘P244601’ and salt-tolerant ‘P1184948’ at 80 mM NaCl compared to salt sensitive ‘Vanda’ and untreated plants. The depressive effect of salt on RWC was recorded at 120 mM NaCl in peanut leaves of all varieties. Under salt stress ‘P1184948’ was observed to have relatively higher tolerance on average of all growth and physiological traits than ‘Vanda’ and P244601’ suggesting that it could be grown in salt-affected soils.

scholarly journals Etanol e ácido cítrico aumentam a longevidade de gérberas cv. Mistique

2019 ◽  
Vol 25 (2) ◽  
pp. 109-118
Author(s):  
Rúbia Kécia Marins Maia ◽  
Edivan Amaro da Silva ◽  
Rafael Mateus Alves ◽  
Maria Aparecida Dos Santos Morais ◽  
Marcos Ribeiro da Silva Vieira ◽  
...  
Keyword(s):  
Citric Acid ◽  
Water Content ◽  
Electrolyte Leakage ◽  
Relative Water Content ◽  
Distilled Water ◽  
Cut Flowers ◽  
Water Control ◽  
Relative Water ◽  
Stem Bending ◽  
Application Methods

Ethanol and citric acid can increase longevity in some cut flowers. However, its use must be extremely careful, with application methods and specific concentrations for each type of cut flower. Thus, the objective was to examine the physico-chemical and physiological changes in Gerbera cv. Mistique cut flowers treated with ethanol (Et) and/or citric acid (CA). Stem were selected and standardized to a length of 35 cm and maintained at 20 ± 2 ºC and RH 65 ± 2%, under continuous lighting. The flowers were subjected to two application methods (pulsing for 48 h and maintenance), using different concentrations of Et (4%, 6%, and 8%) and/or CA (100 and 200 mg L-1) and distilled water (control). A visual assessment and analyses of longevity, stem bending, fresh mass, relative water content, electrolyte leakage, and peroxidase and polyphenol oxidase enzyme activities were performed at every two days. The Et (4%) + CA (100 mg L-1) solution provided the greatest longevity, regardless of the application method. These results were the basis for a third experiment, in which the stems were immersed in pulsing solutions of Et (4%) and/or CA (100 mg L-1) and distilled water (control). The Gerbera flowers under Et + CA solution showed lower fresh mass loss and electrolyte leakage, higher relative water content and a slower increase in polyphenol oxidase and peroxidase activities. This allowed for delayed stem bending and better appearance, resulting in greater longevity compared to the other solutions.

scholarly journals Bacillus subtilis improves maize tolerance to salinity

2018 ◽  
Vol 48 (8) ◽  
Author(s):  
Nathalia Calhabeu Ferreira ◽  
Rita de Cassia Lima Mazzuchelli ◽  
Ana Claudia Pacheco ◽  
Fabio Fernando de Araujo ◽  
Jadson Emanuel Lopes Antunes ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Sodium Chloride ◽  
Plant Growth ◽  
Water Content ◽  
Relative Water Content ◽  
Saline Stress ◽  
Biochemical Responses ◽  
Relative Water ◽  
Four Levels ◽  
Tolerance To Salinity

ABSTRACT: The aim of this study was to evaluate the biochemical responses of maize, under saline stress, inoculated with Bacillus subtilis. Four levels of salinity were assessed: 0mM, 50mM, 100mM, and 200mM of sodium chloride (NaCl). Saline conditions influenced negatively maize growth. However, the inoculation of B. subtilis improved the plant growth at highest level of NaCl. Chlorophyll content decreased while proline increased in inoculated plants submitted to highest salt levels. Also, B. subtilis increased the relative water content in leaves. B. subtilis improves the plant growth under salinity and ameliorates the biochemical damages in maize.

scholarly journals Beneficial Effects of Biochar and Chitosan on Antioxidative Capacity, Osmolytes Accumulation, and Anatomical Characters of Water-Stressed Barley Plants

Agronomy ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 630 ◽  
Author(s):  
Yaser Hafez ◽  
Kotb Attia ◽  
Salman Alamery ◽  
Abdelhalim Ghazy ◽  
Abdullah Al-Doss ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Drought Stress ◽  
Water Content ◽  
Plant Height ◽  
Electrolyte Leakage ◽  
Relative Water Content ◽  
Relative Water ◽  
Number Of Leaves ◽  
The Impact ◽  
Chlorophyll Concentrations

The impact of biochar and chitosan on barley plants under drought stress conditions was investigated during two field experiments. Our results confirmed that drought stress negatively affected morphological and physiological growth traits of barley plants such as plant height, number of leaves, chlorophyll concentrations, and relative water content. However, electrolyte leakage (EL%), lipid peroxidation (MDA), soluble sugars, sucrose and starch contents significantly increased as a response to drought stress. Additionally, 1000 grain weight, grains yield ha−1 and biological yield significantly decreased in stressed barley plants, also anatomical traits such as upper epidermis, lower epidermis, lamina, and mesophyll tissue thickness as well as vascular bundle diameter of flag leaves significantly decreased compared with control. The use of biochar and chitosan led to significant increases in plant height, number of leaves, and chlorophyll concentrations as well as relative water content; nevertheless these treatments led to significant decreases in electrolyte leakage (EL%) and lipid peroxidation (MDA) in the stressed plants. Moreover, anatomical and yield characters of stressed barley plants were improved with application of biochar and chitosan. The results proved the significance of biochar and chitosan in alleviating the damaging impacts of drought on barley plants.

scholarly journals Nanopotassium, Nanosilicon, and Biochar Applications Improve Potato Salt Tolerance by Modulating Photosynthesis, Water Status, and Biochemical Constituents

2022 ◽  
Vol 14 (2) ◽  
pp. 723
Author(s):  
Abdel Wahab M. Mahmoud ◽  
Mahmoud M. Samy ◽  
Hoda Sany ◽  
Rasha R. Eid ◽  
Hassan M. Rashad ◽  
...  
Keyword(s):  
Abscisic Acid ◽  
Plant Growth ◽  
Water Content ◽  
Chlorophyll Content ◽  
Acid Content ◽  
Relative Water Content ◽  
Combined Treatment ◽  
Potato Plants ◽  
Relative Water ◽  
Abscisic Acid Content

Salinity is one of the main environmental stresses, and it affects potato growth and productivity in arid and semiarid regions by disturbing physiological process, such as the photosynthesis rate, the absorption of essential nutrients and water, plant hormonal functions, and vital metabolic pathways. Few studies are available on the application of combined nanomaterials to mitigate salinity stress on potato plants (Solanum tuberosum L. cv. Diamont). In order to assess the effects of the sole or combined application of silicon (Si) and potassium (K) nanoparticles and biochar (Bc) on the agro-physiological properties and biochemical constituents of potato plants grown in saline soil, two open-field experiments were executed on a randomized complete block design (RCBD), with five replicates. The results show that the biochar application and nanoelements (n-K and n-Si) significantly improved the plant heights, the fresh and dry plant biomasses, the numbers of stems/plant, the leaf relative water content, the leaf chlorophyll content, the photosynthetic rate (Pn), the leaf stomatal conductance (Gc), and the tuber yields, compared to the untreated potato plants (CT). Moreover, the nanoelements and biochar improved the content of the endogenous elements of the plant tissues (N, P, K, Mg, Fe, Mn, and B), the leaf proline, and the leaf gibberellic acid (GA3), in addition to reducing the leaf abscisic acid content (ABA), the activity of catalase (CAT), and the peroxidase (POD) and polyphenol oxidase (PPO) in the leaves of salt-stressed potato plants. The combined treatment achieved maximum plant growth parameters, physiological parameters, and nutrient concentrations, and minimum transpiration rates (Tr), leaf abscisic acid content (ABA), and activities of the leaf antioxidant enzymes (CAT, POD, and PPO). Furthermore, the combined treatment also showed the highest tuber yield and tuber quality, including the contents of carbohydrates, proteins, and the endogenous nutrients of the tuber tissues (N, P, and K), and the lowest starch content. Moreover, Pearson’s correlation showed that the plant growth and the tuber yields of potato plants significantly and positively correlated with the photosynthesis rate, the internal CO2 concentration, the relative water content, the proline, the chlorophyll content, and the GA3, and that they were negatively correlated with the leaf Na content, PPO, CAT, ABA, MDA, and Tr. It might be concluded that nanoelement (n-K and n-Si) and biochar applications are a promising method to enhance the plant growth and crop productivity of potato plants grown under salinity conditions.

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