scholarly journals Molecular Characterization of Saffron-Potential Candidates for Crop Improvement

2015 ◽  
Vol 7 (1) ◽  
pp. 81-89 ◽  
Author(s):  
Javid Iqbal MIR ◽  
Nazeer AHMED ◽  
Mudasir Hafiz KHAN ◽  
Taseem Ahmad MOKHDOMI ◽  
Sajad Hussian WANI ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Ssr Markers ◽  
Significant Variation ◽  
Rapd Markers ◽  
Crop Improvement ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Improvement Programs ◽  
Rapd And Issr

In this study, thirty one (31) morphologically distinct selections of saffron crop were used for molecular characterization. Molecular characterization was done through SSR, ISSR and RAPD markers. RAPD and ISSR markers showed significant variation; however, SSR markers did not reveal any variation between the selected clones. The Jaccard’s similarity coefficient ranged from 0.94 to 1.00 with an average of 0.98 among all 31 selections used. Minimum similarity value (0.94) was observed between CITH-S-107 and PAM-S-116 selections. The study provides sufficient knowledge to identify clones with better stigma characteristics for further crop improvement programs.

scholarly journals Molecular Characterization of 12 Mango Germplasm Using RAPD markers

2010 ◽  
Vol 20 (1) ◽  
pp. 91-99
Author(s):  
R. C. Jena ◽  
K. C. Samal ◽  
P. K. Chand ◽  
B. K. Das
Keyword(s):  
Molecular Characterization ◽  
Rapd Markers ◽  
Mangifera Indica ◽  
Pcr Amplification ◽  
Similarity Coefficient ◽  
Group Method ◽  
Base Pairs ◽  
Relationship Analysis ◽  
Pair Group

Randomly amplified polymorphic DNA (RAPD) markers were used for the genetic variation and relationship analysis among 12 Mango (Mangifera indica L.) germplasm. Five oligonucleotide primers were employed to amplify DNA from 12 cultivars. PCR amplification with five primers generated 45 reproducible, clear and distinct bands, out of which 41 bands are considered polymorphic and the remaining four fragments (8.88%)  monomorphic. The size of amplified product ranged from 200 (RPI-5) to 3000 base pairs (RPI-1) with an average of nine bands per primer. The average polymorphism in all the 12 cultivars using the five primers was found to be 91.91%. Among all the primers RPI-2 and RPI-4 have shown 100% polymorphism while RPI-5 was found to be least polymorphism (81.81%). One specific band, namely was found with RPI-5, in a particular variety, Chiratpuri. The UPGMA (Unweighted Pair Group Method of Arithmetic Mean) dendrogram based on Jaccard’s similarity coefficient segregated the 12 mango germplasm into two clusters. Langra, Chiratpuri, Pravasankar, Alphanso, Sindhu and Kesar formed one cluster and rest six mango germplasm grouped together into another cluster. Sindhu and Alphanso cultivar pair was very close to each other with highest similarity coefficient (0.78), which was comparatively higher than all other cultivar pairs. On the other hand, Pravasankar and Neelam cultivar pair was more distinct to each other with the lowest intervarietal similarity coefficient 0.38. This study showed clearly that cultivars from Orissa unveiled maximum diversity and indicated the potential of RAPD markers for the identification of management of mango germplasm for breeding purposes.  Key words: Molecular characterization, Mango germplasm, Dversity  D.O.I. 10.3329/ptcb.v20i1.5972 Plant Tissue Cult. & Biotech. 20(1): 91-99, 2010 (June)

Molecular characterization of jasmine genotypes using RAPD and ISSR markers

2020 ◽  
Vol 77 (1) ◽  
pp. 149
Author(s):  
Sanchita Ghosh ◽  
M. Ganga ◽  
K. Soorianathasundaram ◽  
Ajit Kumar
Keyword(s):  
Molecular Characterization ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals Molecular characterization of potato cultivars using SSR markers

2011 ◽  
Vol 29 (4) ◽  
pp. 542-547 ◽  
Author(s):  
Patrícia Favoretto ◽  
Elizabeth Ann Veasey ◽  
Paulo César Tavares de Melo
Keyword(s):  
Molecular Characterization ◽  
Ssr Markers ◽  
Simple Sequence Repeat ◽  
Binary Data ◽  
Potato Crop ◽  
Similarity Coefficient ◽  
High Similarity ◽  
Commercial Cultivars ◽  
Simple Sequence

The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for primer STM0019a. Of the 46 alleles, only five were monomorphic, therefore presenting 89.1% polymorphism. The polymorphism information content (PIC) varied from 0.13 to 0.86, with an average of 0.54. The Jaccard´s coefficient varied from 0.41 to 0.93, showing high genetic variability among accessions. Two possible duplicates [Atlantic (Canada) and Atlantic (Chile), and Colorado and Ágata (EPAMIG) (duplicates with these SSRs, which did not separate them)] were identified. High similarity was also shown by cultivars Chipie and Melodie (EPAMIG), Voyager and Gourmandine (EPAMIG), Eole and Caesar (EPAMIG), and Cupido and Santé (Pirassu). The most genetically divergent accessions (Lady Rosetta and HPC-7B) were also identified.

scholarly journals Molecular Characterization of Kiwifruit (Actinidia) Cultivars and Selections Using SSR Markers

2004 ◽  
Vol 129 (3) ◽  
pp. 374-382 ◽  
Author(s):  
Yiqi Zhen ◽  
Zuozhou Li ◽  
Hongwen Huang ◽  
Ying Wang
Keyword(s):  
Genetic Diversity ◽  
Molecular Characterization ◽  
Ssr Markers ◽  
Rapd Markers ◽  
Reliable Method ◽  
Cultivar Identification ◽  
Direct Count ◽  
Polymorphic Loci ◽  
Single Genotype

Forty-eight kiwifruit cultivars and selections, representing more than 90% of total world kiwifruit production, were investigated using nine SSR markers to establish genetic identities, and evaluate genetic diversity and relatedness. These nine SSRs were polymorphic and a total of 213 alleles were detected, resulting in a mean number of 23.7 alleles per locus, ranging from nine to 38 alleles. One hundred and thirty-three alleles were found to be common to both A. chinensis and A. deliciosa, while 33 and 36 were specific to A. chinensis and A. deliciosa, respectively. In addition, 34 alleles were specific to one single genotype and provided a set of valuable alleles for cultivar identification. A single SSR locus UDK 96-414 could differentiate all 48 genotypes except two presumable clones. Mean number of alleles per locus (A), percentage of polymorphic loci (P), and direct count heterozygosity (Ho) assessed for each genotype over all loci revealed considerable differences among these 48 genotypes. On average, A = 2.6, P = 89.4% and Ho = 0.546 were found in A. chinensis cultivars, while A = 3.5, P = 97.0% and Ho = 0.671 in A. deliciosa cultivars. Consensus fingerprint profiling using SSR markers is a useful and reliable method for establishing genetic identities of kiwifruit cultivars and selections. It also improves evaluation effectiveness of genetic diversity and relatedness compared to RAPD markers.

Molecular Characterization of Brinjal (Solanum melongena L) Cultivars using RAPD and ISSR Markers

2009 ◽  
Vol 18 (2) ◽  
pp. 189-195 ◽  
Author(s):  
Shailesh K. Tiwari ◽  
J. L. Karihaloo ◽  
Nowsheen Hameed ◽  
Ambika B. Gaikwad
Keyword(s):  
Molecular Characterization ◽  
Solanum Melongena ◽  
Issr Markers ◽  
Rapd And Issr

scholarly journals Genetic diversity of chickpea genotypes as revealed by ISSR and RAPD markers

Genetika ◽  
2017 ◽  
Vol 49 (2) ◽  
pp. 415-423
Author(s):  
Saida Hasanova ◽  
Zeynal Akparov ◽  
Alamdar Mammadov ◽  
Litfer Amirov ◽  
Sevda Babayeva ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Rapd Markers ◽  
Diversity Index ◽  
Issr Markers ◽  
Similarity Coefficient ◽  
Parental Lines ◽  
Spss Software ◽  
Rapd And Issr ◽  
Combined Data ◽  
Issr Primers

Genetic diversity of 62 chickpea accessions was studied using 8 ISSR and 11 RAPD primers. In the study RAPD primers detected more polymorphism (98%) than the ISSR primers (80%). Genetic diversity index was high (0.73 for ISSR and 0.85 for RAPD) for each of these marker systems. Cluster analysis performed from both separate and combined data of RAPD and ISSR markers using SPSS software package. Jaccard?s similarity coefficient for 62 chickpea genotypes was 0.65. Cluster analyses based on combined data generated a dendrogram that separated genotypes into 11 clusters. Four clusters contained only one genotype showing the genetic uniqueness of these accessions. The studied chickpea collection has been proved to constitute a rich source of biodiversity as revealed by RAPD and ISSR markers. Crossing between distantly related genotypes is expected to yield more vigorous plants constituting much of the different traits contained in the two parental lines.

Molecular Characterization of Chickpea (Cicer ArietinumL) Through Rapd and Issr Markers

2015 ◽  
Vol 15 (2) ◽  
pp. 277 ◽  
Author(s):  
Mukesh Kumar ◽  
Navneet Kumar ◽  
Sunil Malik ◽  
Arvind Kumar ◽  
Vipin Kumar
Keyword(s):  
Molecular Characterization ◽  
Issr Markers ◽  
Rapd And Issr

Collection and characterization of banana gene pools (Musa spp.) in Manipur (N.E. India) using PCR–RFLP and RAPD and ISSR markers

2021 ◽  
Author(s):  
Warepam Amuchou Singh ◽  
Nandeibam Samarjit Singh ◽  
Elangbam Julia Devi ◽  
Pratap Jyoti Handique ◽  
Huidrom Sunitibala Devi
Keyword(s):  
Issr Markers ◽  
Gene Pools ◽  
Musa Spp ◽  
Pcr Rflp ◽  
Rapd And Issr

SSR based molecular profiling of elite cultivars of basmati rice (Oryza sativa L.)

2021 ◽  
Vol 16 (12) ◽  
pp. 55-63
Author(s):  
Ashwani Yadav ◽  
Anubhuti Sharma ◽  
Ashwani Kumar ◽  
Renu Yadav ◽  
Rajendra Kumar
Keyword(s):  
Ssr Markers ◽  
Oryza Sativa L ◽  
Research Work ◽  
Similarity Coefficient ◽  
Basmati Rice ◽  
Bank Management ◽  
Genomic Study ◽  
Leaf Tissues ◽  
Maximum Similarity

The experiment was conducted to assess the available genetic variability amongst Indian Basmati rice and identify co-dominant and reproducible robust simple sequence repeat markers for drought resistance and their utilization in marker assisted selection for developing drought resistant / tolerant aromatic rice cultivars in various parts of India as well as in world. DNA was isolated from fresh and young leaf tissues of 35 cultivars of basmati rice using the CTAB procedure of Doyle and Doyle with slightly modifications. The DNA was further quantified by spectrophotometer at 260 nm and 280 nm. The quality and quantity of DNA were checked by agarose gel electrophoresis. Out of 60 SSR markers, 18 were found to be polymorphic and the rest 42 primers were monomorphic. One of the main purposes of SSR markers in genomic study is the characterization of genetic resources to help gene bank management. The informative (18) SSR markers were capable to discriminate the entire cultivars of basmati rice used in this research work. A total of 52 alleles at 18 loci could be scored. The allelic richness per locus diversified from 2 to 5 with an average of 2.89 alleles per locus. The amplitude of Polymorphism Information Content (PIC) value is 0.066 (RM 1068) to 0.730 (RM 1059) with an average of 0.505. The Jaccard’s similarity coefficient ranged from 0.79 to 0.95. Polymorphic finding content showed a positive correlation (r =0.69) with number of alleles at the SSR locus. However it is recommended that SSR markers can be efficiently utilized for this purpose. The maximum similarity coefficient was observed between Jeerakasab and Kalanamak with a coefficient value of 95% and the minimum similarity was found between Jeerakasab and Pusa basmati-1 with a coefficient value of 79%.

scholarly journals Characterization of genetic structure of alfalfa (Medicago sp.) from trans-Himalaya using RAPD and ISSR markers

2011 ◽  
Vol 10 (42) ◽  
pp. 8176-8187 ◽  
Author(s):  
Raj Xavier Janifer ◽  
Kumar Jitendra ◽  
Bihari Srivastava Ravi
Keyword(s):  
Genetic Structure ◽  
Issr Markers ◽  
Rapd And Issr
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