scholarly journals Genetic Variation of Isozyme Polyphenol Oxidase (PPO) Profiles in Different Varieties of Capsicum annuum L.

2013 ◽  
Vol 5 (4) ◽  
pp. 454-457
Author(s):  
Owk ANIEL KUMAR ◽  
Tamminana RUPAVATHI ◽  
Sape SUBBA TATA
Keyword(s):  
Capsicum Annuum ◽  
Polyphenol Oxidase ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Native Page ◽  
Chilli Pepper ◽  
Upgma Dendrogram ◽  
Low Genetic Diversity ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Genus Capsicum

The genus Capsicum commonly known as chilli pepper is a major spice crop and is of cosmopolitan in distribution. Native polyacrylamide gel electrophoresis (Native PAGE) was used to study the polyphenol oxidase (PPO) isozyme variation in 21 varieties of Capsicum annuum L. A maximum of 4 PPO bands were scored in five varieties i.e., Ca14, Ca15, Ca16, Ca19 & Ca20, while the minimum (2 bands) was observed in four varieties (Ca3, Ca10, Ca13 & Ca17). 15 pair wise combinations showed highest average per cent similarity (100%) and the UPGMA dendrogram represented low genetic diversity. The present study revealed that considerable intraspecific differences were found in the varieties. Thus the results obtained could be used in fingerprinting the genotypes.

scholarly journals APPLICATION OF GRAPHENE OXIDE ON APTAMER-BASED BIOSENSOR DEVELOPMENT FOR AUTHENTICATION OF GELATIN

2019 ◽  
pp. 254-258
Author(s):  
HARI WIDADA ◽  
ABDUL ROHMAN ◽  
RIRIS ISTIGHFARI JENIE ◽  
SISMINDARI .
Keyword(s):  
Graphene Oxide ◽  
Gel Electrophoresis ◽  
Dna Analysis ◽  
Selection Process ◽  
Polyacrylamide Gel Electrophoresis ◽  
Pcr Amplification ◽  
Native Page ◽  
Sharp Separation ◽  
Aptamer Selection ◽  
Native Polyacrylamide Gel Electrophoresis

Objective: The objective of this study was to perform aptamer selection using systematic evolution of ligands by exponential enrichment (SELEX) method which assisted by graphene oxide against target of porcine gelatin (non-halal gelatin). Methods: The aptamer selection was carried out using SELEX method without target immobilization. Selection of aptamer capable of binding porcine gelatin by applying grafen oxide (GO) was known as GO-SELEX. The selection process was initially carried out by incubation of single-stranded DNA (ssDNA) libraries targeting on porcine gelatin with the addition of graphene oxide. The selected ssDNA was then purified by several stages namely; symmetric PCR amplification, purification of products with DNA purification kits, asymmetric PCR amplification, and continued purification of DNA with native PAGE. The analysis of each stage was done by agarose gel electrophoresis. Results: the results showed that aptamer targeting porcine DNA could be selected. This was indicated by the results of DNA analysis using native polyacrylamide gel electrophoresis (PAGE) in which sharp separation band with a base length equivalent to the marker of the ssDNA library (about 80 base pair) was obtained. Conclusion: Aptamer targeting on porcine gelatin has been successfully developed using GO-SELEX method. GO can increase selectivity in developing aptamer which will be used as a biosensor to detect porcine gelatin. The method could be proposed as a standard of apatamer based method for porcine gelatin detection on halal products authentication.

Actinobacterial chitinase-like enzymes: profiles of rhizosphere versus non-rhizosphere isolates

2003 ◽  
Vol 49 (11) ◽  
pp. 683-698 ◽  
Author(s):  
Ana C Gonzalez-Franco ◽  
Lee A Deobald ◽  
Aaron Spivak ◽  
Don L Crawford
Keyword(s):  
Cell Wall ◽  
Gel Electrophoresis ◽  
Rhizosphere Soil ◽  
Polyacrylamide Gel Electrophoresis ◽  
Hydrolytic Enzymes ◽  
Fungal Cell ◽  
Native Page ◽  
Substrate Preferences ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Over Time

The objective of this study was to determine if antifungal actinomycetes isolated from rhizosphere and non-rhizosphere soils exhibit different chitinase-like production and (or) induction patterns. Selected isolates from both habitats were compared. Chitinase-like levels and isoform characteristic patterns were evaluated over time in culture fluids of isolates grown on media containing different combinations of colloidal chitin and fungal cell wall (FCW) preparation. Supernatants were also subjected to native and non-native polyacrylamide gel electrophoresis (PAGE), using glycol chitin amended gels. For non-native PAGE, protein samples were denatured by two different approaches. Multiple active bands, ranging from 20 to 53 kDa and present in varying amounts, were detected in gels for most strains. Different substrate preferences were observed among strains, and different chitinase-like enzymes were produced, depending upon the substrate combinations used. The presence of FCW in the medium induced specific chitinase-like enzymes not observed otherwise. Enzymatic activities and profiles of the isolates, however, were strain and substrate specific rather than habitat specific. However, a sagebrush rhizosphere soil had a larger actinomycete community with higher chitinolytic activities than the nearby bulk soil. The use of PAGE to compare chitinase-like proteins induced in media with and without FCW was useful for identifying chitinase-like enzymes potentially involved in antifungal activity.Key words: chitinase, actinomycetes, hydrolytic enzymes, rhizosphere, antifungal.

scholarly journals Analysis of Several Popular Cultivars of Madagascar Periwinkle (Catharanthus roseus (L.) G. Don.) using Biochemical Markers

2013 ◽  
Vol 5 (4) ◽  
pp. 458-461
Author(s):  
Owk ANIEL KUMAR ◽  
Sape S. TATA ◽  
Kancharla PAVAN KUMAR
Keyword(s):  
Catharanthus Roseus ◽  
Gel Electrophoresis ◽  
Biochemical Markers ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cultivar Differences ◽  
Madagascar Periwinkle ◽  
Large White ◽  
Electrophoretic Patterns ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Isozyme Polymorphism

Band designs of esterase (EST), peroxidase (PO) and polyphenol oxidase (PPO) isozymes in several selected cultivars of Catharanthus roseus by using native polyacrylamide gel electrophoresis (PAGE) were investigated in this study. It was confirmed that cultivar differences in isozyme polymorphism can be revealed by applied electrophoretic patterns. Three isozyme systems produced a total of 16 bands with polymorphism ranged from 66.6-100%. Considering the patterns of isozyme variations in the five cultivars of Catharanthus roseus, it is evident that the cultivar ‘First kiss coral’ displayed crimson red petal with large white eye’ displayed demarked profiles of EST, PO and PPO isozymes than other cultivars. This is the first report on isozyme polymorphism in members of the Cathanarathus roseus (L.) G. Don.

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