scholarly journals Effect of Basal Medium, Explants Size and Density on the In Vitro Proliferation and Growth of Date Palm (Phoenix dactylifera L.) Cultivar ‘16-bis’

2013 ◽  
Vol 5 (3) ◽  
pp. 332-337 ◽  
Author(s):  
Mouaad Amine MAZRI
Keyword(s):  
Date Palm ◽  
Woody Plant ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Phoenix Dactylifera ◽  
In Vitro Proliferation ◽  
Phoenix Dactylifera L ◽  
Murashige And Skoog Medium ◽  
Woody Plant Medium

The effect of basal medium, explant size and density on shoot multiplication, growth, rooting and acclimatization of date palm cv. ‘16-bis’ was evaluated. Bud clusters of different sizes (2, 3, 4 and 5 buds per cluster) were cultured at density of 1, 2, 3 and 4 clusters on Murashige and Skoog medium (MS), woody plant medium (WPM) and Nitsch medium (NM) supplemented with 0.5 mg/L 2-naphthoxyacetic acid and 0.5 mg/L kinetin for three months (multiplication phase). Separated shoots of different sizes (

scholarly journals PERBANYAKAN AKASIA HIBRIDA (Acacia mangium × Acacia auriculiformis) MELALUI SUBKULTUR BERULANG

2020 ◽  
Vol 7 (1) ◽  
Author(s):  
Yelnititis Yelnititis ◽  
Sri Sunarti
Keyword(s):  
Woody Plant ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Shoot Length ◽  
Acacia Auriculiformis ◽  
Shoot Induction ◽  
Benzyl Adenine ◽  
Single Node ◽  
Woody Plant Medium

In vitro culture is a promising technique for mass propagation of high-value species. Study of propagation for Acacia hybrid (A. mangium x A. auriculiformis) through this technique has been conducted using single node stem from seedlings as explants. Growth medium used was modified Murashige and Skoog (MS), basal medium Woody Plant Medium (WPM), and Gamborg (B5) supplemented. The study was conducted in two stages, namely shoot induction and shoot multiplication. The treatment tested was the Benzyl Adenine (BA) supplementation at the concentration of 0.3; 0.7; and 1.0 mgL-1 of. Observation was conducted on the frequency of shoot induction, number of shoot, shoot length and visual performance of the culture. The result showed that treatment of BA 0.7 mgL-1 on modified MS medium is the best for shoot induction, shoot multiplication and visual performace of the culture. The average of number of shoot was 2.6; 5.0 and 7.7 shoots on the first three consecutive subcultures. Changing to different basal medium on the fourth subculture showed that the treatment of BA 0.7 mgl-1 is the best condition for shoot regeneration (12.60 shoots) and shoot length (6.97 cm). The culture from this treatment showed the best visual morphological performance.Keywords:Acacia hybrid; multiplication; subculture; in vitro; BA. ABSTRAKKultur in vitro merupakan suatu teknik yang menjanjikan untuk perbanyakan massal spesies-tanaman bernilai tinggi. Penelitian perbanyakan akasia hibrid (A. mangium x A. auriculiformis) melalui kulturin vitro telah dilakukan dengan menggunakan eksplan berupa batang satu buku yang berasal dari anakan. Media tumbuh yang digunakan adalah media dasar Murashige dan Skoog (MS) yang sudah dimodifikasi, media dasar Woody Plant Medium (WPM), dan Gamborg (B5). Penelitian dilakukan dalam dua tahap yaitu induksi tunas dan perbanyakan tunas. Perlakuan yang diuji adalah penggunaan Benzyl Adenine (BA) dengan konsentrasi 0,3; 0,7 dan 1,0 mg L-1. Pengamatan dilakukan terhadap waktu induksi tunas, jumlah tunas, tinggi tunas dan penampilan biakan secara visual. Hasil penelitian menunjukkan bahwa penggunaan BA 0,7 mg L-1 pada media MS modifikasi merupakan perlakuan terbaik untuk induksi tunas, perbanyakan tunas, tinggi tunas, dan kondisi biakan secara visual. Jumlah rata-rata tunas yang dihasilkan dari perlakuan ini adalah 2,6; 5,0 dan 7,7 tunas pada subkultur pertama, kedua dan ketiga. Pada penggunaan media dasar berbeda pada subkultur keempat menunjukkan bahwa perlakuan BA 0,7 mg L-1 merupakan perlakuan terbaik dengan jumlah tunas sebanyak 12,60 tunas dan rata-rata tinggi tunas 6,97 cm. Biakan yang dihasilkan dari perlakuan tersebut mempunyai penampilan yang baik dan normal.

scholarly journals Effect of glutathione (GSH) on Date palm (Phoenix dactylifera L.) micropropagation

2020 ◽  
Vol 47 (1) ◽  
pp. 64-69
Author(s):  
Ahmed Madi Waheed Al-Mayahi ◽  
Osama Nazim Jafar ◽  
Khaun Ali Mohsen
Keyword(s):  
Biochemical Analysis ◽  
Date Palm ◽  
Shoot Multiplication ◽  
Phoenix Dactylifera ◽  
Callus Growth ◽  
Control Treatment ◽  
Pal Activity ◽  
Phoenix Dactylifera L ◽  
Soluble Phenols

AbstractThe investigation was carried out to evaluate the influence of glutathione (GSH: levels 0, 0.1, 0.5 1.0, and 2.0 mM) on the callus growth, shoot multiplication and phytochemicals of in vitro shoots of the Date palm cv. Barhee. The optimum concentration of GSH was 1.0 mM. Such concentration improved the callus growth and increased its weight to 312.0 mg, the addition of this substance of the same concentration level showed the highest response rate and the number of shoots per jar (73.34 ± 2.69 and 8.83 ± 0.80 shoots/jar, respectively). Also, the 1.0 GSH application resulted in reducing the percentage of browning to 0.0 compared to the other concentrations. The results of the biochemical analysis revealed that treatments GSH of 0.1, 0.5 and 1.0 mM reduced the total soluble phenols compound (TSPC). These treatments were also more effectively reducing peroxidase (POD) and phenylalanine ammonia-lyase (PAL) activity than the concentrations of 2 mM or 0 (control treatment).

scholarly journals Effect of aluminum on the growth of the in vitro culture tissues of the date palm (Phoenix dactylifera L.) cv. Um-Aldehin

2019 ◽  
Vol 46 (2) ◽  
pp. 164-169 ◽  
Author(s):  
Ahmed Madi Waheed Al-Mayahi
Keyword(s):  
Aluminum Chloride ◽  
Date Palm ◽  
Shoot Multiplication ◽  
Phoenix Dactylifera ◽  
Control Treatment ◽  
High Concentration ◽  
Phoenix Dactylifera L ◽  
Calcium Magnesium ◽  
Nitrogen Phosphorus

AbstractThis study was carried out to evaluate the effect of different concentrations of aluminum: (25, 50 and 100 mg l−1 of aluminum chloride AlCl3. 6H2O) on the enhancement of shoot multiplication, phytochemicals, as well as, antioxidant enzyme activity of in vitro cultures of the date palm cv. Um-Aldehin. The addition of aluminum in a concentration of 50 mg l−1 to the medium exhibited most effects on shoots regeneration and this substance also increased the number of shoots per a jar (73.34% and 8.2 shoots/jar, respectively), compared to the other concentrations, including the control treatment. A biochemical analysis of date palm shoots revealed that the high concentration of aluminum chloride (100 mg l−1) led to a significant accumulation of the total proline content, and to a high activity of ascorbate peroxidase, as well as a significant reduction in nitrogen, phosphorus, calcium, magnesium and chlorophyll, compared to the concentration of 50 mg l−1 for which there were recorded the highest contents of the mentioned elements, together with the highest content of chlorophyll in leaves.

scholarly journals 2,4-D induction of somaclonal variations in in vitro grown date palm (Phoenix dactylifera L. cv Barhee)

2021 ◽  
Author(s):  
Emna Baklouti ◽  
Thierry Beulé ◽  
Amèni Nasri ◽  
Amal Ben Romdhane ◽  
Riadh Drira ◽  
...  
Keyword(s):  
Date Palm ◽  
Specific Activity ◽  
Phoenix Dactylifera ◽  
Proline Content ◽  
Epigenetic Changes ◽  
Negative Effects ◽  
Somaclonal Variations ◽  
Phoenix Dactylifera L ◽  
Free Media

Abstract The present study is a part of a program designed at improving the date palm, Phoenix dactylifera L. cv. Barhee, through induced somaclonal variation. In this work, caulogenic cultures were subcultured on MS media supplemented with 0, 1, 5, 10, 20 and 40 mg L− 1 2,4-D in order to induce genetic and epigenetic variations. The highest doses of 2,4-D were found to induce severe negative effects on in vitro cultures, although some tissues were able to survive and to produce calli with high morphogenetic capacities. Our analysis showed some significant effect of 2,4-D on several physiological parameters. Indeed, chlorophyll and growth rates were found to drastically decrease while proline content increased from 535 nmol g− 1 to 2973 nmol g− 1 FW when 40 mg L− 1 2,4-D were used. In vitro cultures showed several signs of oxidative stress, such as high levels of hydrogen peroxide (H2O2) and malondialdehyde (MDA); likewise, the specific activity of several antioxidant enzyme was found to increase. Plant regeneration from in vitro cultures treated with 2,4-D was obtained after subculturing explants onto PGR-free media. The ISSR analysis of 2,4-D-treated material showed that this plant growth regulator (PGR) induced measurable genetic variations. The global DNA methylation rates (GMR) as estimated through the HPLC analysis of nucleosides also confirmed the presence of epigenetic changes caused by 2,4-D as GMRs increased from 13.8–18.93%.

scholarly journals In vitro Morphogenesis of Arabian Date Palm (Phoenix dactylifera L.)

2014 ◽  
Vol 23 (2) ◽  
pp. 211-219
Author(s):  
Md. Abul Kalam Azad ◽  
Hasnatul Arefin ◽  
Md. Amzad Hossain
Keyword(s):  
Date Palm ◽  
Callus Formation ◽  
Shoot Proliferation ◽  
Phoenix Dactylifera ◽  
Shoot Induction ◽  
In Vitro Morphogenesis ◽  
Shoot Initiation ◽  
Phoenix Dactylifera L ◽  
Young Leaves

After inoculation of young leaves of date palm offshoot required about six months to complete the morphogenesis process. Fourteen weeks were required for embryogenic callus formation under continuous dark condition and nine weeks for shoot initiation (under 16/8 h light/dark). The highest number of explants (80%) produced callus in modified MS containing 5 mg/l 2,4-D + 2 mg/l 2ip. Sixty per cent of explants produced callus in the modified medium containing 5 mg/l 2,4-D + 5 mg/l NAA. while only 50 per cent of the explants formed callus in the same medium when supplemented with only 5 mg/l 2,4-D. The induced calli were transferred to modified MS for shoot proliferation. A combination of two cytokines showed better performance than single ones in shoot induction. The highest percentage (70) of shoot developed in modified MS containing 2 mg/l BAP + 1 mg/l Kn. Forty per cent shoot induction was found in the same medium supplemented with 2 mg/l of BAP. Thirty per cent shoot formed in MS containing 1 mg/l of Kn. The shoots were subcultured at three- four week intervals throughout culture duration. D. O. I. http://dx.doi.org/10.3329/ptcb.v23i2.17522 Plant Tissue Cult. & Biotech. 23(2): 211-219, 2013  (December)

CRYOPRESERVATION OF DATE PALM (PHOENIX DACTYLIFERA L.) CULTURED IN VITRO

2007 ◽  
pp. 283-291 ◽  
Author(s):  
S.A. Bekheet ◽  
H.S. Taha ◽  
M.E. Solliman ◽  
N.A. Hassan
Keyword(s):  
Date Palm ◽  
Phoenix Dactylifera ◽  
Phoenix Dactylifera L
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