scholarly journals Assessment of Genetic Diversity of Promising Castor Been (Ricinus communis L.) Genotypes in Nigeria

2019 ◽  
Vol 11 (3) ◽  
pp. 467-474
Author(s):  
Bolaji Zuluqurineen SALIHU ◽  
Olamide Ahmed FALUSI ◽  
Adeyinka Olufemi ADEPOJU ◽  
Ibrahim Wasiu AROLU ◽  
Oladipupo Yusuf DAUDU ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
Gene Diversity ◽  
Ricinus Communis ◽  
Polymorphic Information Content ◽  
Block Design ◽  
Genetic Material ◽  
Morphological Data ◽  
Research Attention ◽  
Ricinus Communis L

Castor oil plant (Ricinus communis L.) is an important oil crop with little research attention in Nigeria. In the present research, extent of genetic diversity among 20 Nigerian castor genotypes was determined using morphological descriptors and molecular markers. The genotypes were laid out on a randomized complete block design with three replicated plots. Molecular genotyping of the genotypes was carried out using genomic Simple Sequence Repeats (SSR). The genotypes revealed high divergence in seed colour, seed shape, seed mottle, seed caruncle and seed sizes. Seedling establishment varied from 70.18% (in Acc. 006) to 93.25% (Acc. 001) with average mean of 81.53%. Raceme length ranged from 15.90 cm to 29.54 cm with population mean of 20.80 cm. The highest seed yield (1222.98 kg/ha) was recorded in Acc. 001 and the least (611.46 kg/ha) was observed in Acc. 006. Seed oil content varied between 32.15% in Acc. 042 and 54.03% in Acc. 006. Agglomerative cluster dendrogram constructed from morphological data showed random distribution of the genotypes into three cluster groups irrespective of the sources/collection points. The genetic diversity based on SSR Marker Analysis revealed high average expected heterozygosity (0.74), Polymorphic information content (0.68), Nei’s gene diversity index (0.72) and Shannon's Information index (1.43). The dendrogram constructed from molecular data grouped the twenty genotypes into three groups at coefficient of 0.34. From these findings, it showed that the twenty genotypes evaluated are divergent in nature and they could serve as good genetic material for castor breeding in Nigeria.

scholarly journals Genetic Diversity of Castor Bean (Ricinus communis L.) Revealed by ISSR and RAPD Markers

Agronomy ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 457
Author(s):  
HyokChol Kim ◽  
Pei Lei ◽  
Aizhi Wang ◽  
Shuo Liu ◽  
Yong Zhao ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Castor Bean ◽  
Genetic Similarity ◽  
Rapd Markers ◽  
Ricinus Communis ◽  
Similarity Coefficient ◽  
Morphological Data ◽  
Oilseed Crop ◽  
Ricinus Communis L ◽  
Polymorphic Bands

Castor (Ricinus communis L.), known as castor oil plant or castor bean, is a non-edible oilseed crop. In the present study, the genetic diversity among 54 samples (3 wild and 51 cultivated) collected worldwide was evaluated using inter-simple sequence repeats (ISSRs) and random amplified polymorphic DNA (RAPD) markers. A total of 9 ISSR primers produced 83 high-resolution bands with 61 (74.53%) as polymorphic. The percentage of polymorphic bands per primer and the genetic similarity coefficient ranged from 54.55% (UBC-836) to 100% (UBC-808) and from 0.74 to 0.96, respectively. A total of 11 out of 20 RAPD primers amplified unique polymorphic products with an average percentage of polymorphic bands of 60.98% (56 polymorphic bands out of a total of 90 bands obtained). The percentage of polymorphic bands per primer ranged from 25% (OPA-02 and B7) to 90.91% (B21) with the genetic similarity coefficient ranging from 0.73 to 0.98. The unweighted pair group method with arithmetic averages (UPGMA) dendrogram using two molecular markers divided 54 castor genotypes into three groups. Furthermore, based on morphological data, all 54 castor varieties were grouped into three main clusters. The genetic diversity analysis based on two molecular makers showed that most varieties from China were closely related to each other with three varieties (GUANGDONGwild, ZHEJIANGWild, and HANNANWild) belonging to a wild group separated from most of the cultivated castor samples from China, India, France, and Jordan. These results suggested that the cultivated castor contains a narrow genetic base. Accordingly, we recommend that wild castor genetic resources be introduced for breeding novel castor varieties. Furthermore, the Vietnam, Malaysia, Indonesia, and Nigeria accessions were clustered into the same group. The results of principal coordinate analysis (PCoA) and UPGMA cluster analysis were consistent with each other. The findings of this study are important for future breeding studies of castor.

scholarly journals Genetic diversity in Tunisian castor genotypes (Ricinus communis L.) detected using RAPD markers

10.5219/1116 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 294-300 ◽  
Author(s):  
Martin Vivodík ◽  
Ezzeddine Saadaoui ◽  
Želmíra Balážová ◽  
Zdenka Gálová ◽  
Lenka Petrovičová
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Rapd Markers ◽  
Ricinus Communis ◽  
Polymorphic Information Content ◽  
Biodiesel Production ◽  
Group Method ◽  
Crop Species ◽  
Ricinus Communis L ◽  
The World

Castor (Ricinus communis L.) is a plant that is commercially very important to the world. It is produced in about 30 countries lying in the tropical belt of the world. It is an important plant for production of industrial oil. Assessment of genetic diversity of a crop species is a prerequisite to its improvement; hence it is important to identify the genetic diversity of castor genetic resources for development of improved cultivars. The present study is focused on estimation of genetic distance between 56 Tunisian castor genotypes, based on 18 RAPD markers. Seeds of castor were obtained from the University of Carthage, National Institute of Research in Rural Engineering, Waters and Forests (INRGREF), Regional Station of Gabès, Tunisia. The ricin genotypes were obtained from 12 regions of Tunisia. The efficacy of the RAPD technique in this study is further supported by the obtained PIC values of the primers used in the analysis. PCR amplification of DNA using 18 primers for RAPD analysis produced 145 DNA fragments that could be scored in all 56 genotypes of Tunisian castor. The number of amplified fragments varied from 3 (OPE-07) to 13 (SIGMA-D-01), and the amplicon size ranged from 100 to 1500 bp. Of the 145 amplified bands, 145 were polymorphic, with an average of 8.11 polymorphic bands per primer. The lowest values of polymorphic information content were recorded for RLZ 9 (0.618) and the the highest PIC values were detected for OPD-08 (0.846) with an average of 0.761. A dendrogram was constructed from a genetic distance matrix based on profiles of the 18 RAPD primers using the unweighted pair-group method with the arithmetic average (UPGMA). According to analysis, the collection of 56 Tunisian castor genotypes were clustered into five main clusters. Genetically the closest were four genotypes from cluster 1 (BT-1 – S-5 and K-1 – N-3). Knowledge of the genetic diversity of castor can be used in future breeding programs for increased oil production to meet the ever increasing demand of castor oil for industrial uses as well as for biodiesel production.

scholarly journals Molecular diversity analysis in hexaploid wheat (Triticum aestivum L.) and two Aegilops species (Aegilops crassa and Aegilops cylindrica) using CBDP and SCoT markers

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ghazal Ghobadi ◽  
Alireza Etminan ◽  
Ali Mehras Mehrabi ◽  
Lia Shooshtari
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Crop Improvement ◽  
Wild Relatives ◽  
Resolving Power ◽  
Start Codon ◽  
Aegilops Cylindrica ◽  
Aegilops Crassa

Abstract Background Evaluation of genetic diversity and relationships among crop wild relatives is an important task in crop improvement. The main objective of the current study was to estimate molecular variability within the set of 91 samples from Triticum aestivum, Aegilops cylindrica, and Aegilops crassa species using 30 CAAT box–derived polymorphism (CBDP) and start codon targeted (SCoT) markers. Results Fifteen SCoT and Fifteen CBDP primers produced 262 and 298 fragments which all of them were polymorphic, respectively. The number of polymorphic bands (NPB), polymorphic information content (PIC), resolving power (Rp), and marker index (MI) for SCoT primers ranged from 14 to 23, 0.31 to 0.39, 2.55 to 7.49, and 7.56 to 14.46 with an average of 17.47, 0.34, 10.44, and 5.69, respectively, whereas these values for CBDP primers were 15 to 26, 0.28 to 0.36, 3.82 to 6.94, and 4.74 to 7.96 with a mean of 19.87, 0.31, 5.35, and 6.24, respectively. Based on both marker systems, analysis of molecular variance (AMOVA) indicated that the portion of genetic diversity within species was more than among them. In both analyses, the highest values of the number of observed (Na) and effective alleles (Ne), Nei’s gene diversity (He), and Shannon’s information index (I) were estimated for Ae. cylindrica species. Conclusion The results of cluster analysis and population structure showed that SCoT and CBDP markers grouped all samples based on their genomic constitutions. In conclusion, the used markers are very effective techniques for the evaluation of the genetic diversity in wild relatives of wheat.

Evaluation of Genetic Diversity in Castor (Ricinus communis L.) using RAPD Markes

2011 ◽  
Vol 343-344 ◽  
pp. 981-987
Author(s):  
Feng Juan Li ◽  
Chang Lu Wang ◽  
Dong He ◽  
Ya Qiong Liu ◽  
Mian Hua Chen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Genetic Distance ◽  
Oil Content ◽  
Rapd Markers ◽  
Rapd Analysis ◽  
Ricinus Communis ◽  
Major Group ◽  
Ricinus Communis L ◽  
Polymorphic Bands

RAPD markers are used to study the genetic diversity of the main planting on 37 castor varieties widely cultivated in china according to the oil content and other characteristic of different castor varieties. Genetic distance of 37 Chinese castor varieties is studied by RAPD markers analysis. RAPD analysis shows that a total of 122 bands are amplified from random primers of 20 S series, including 71 polymorphic bands with polymorphic rate of 58.20%. 37 castor beans are divided into four major groups in the phylogenetic tree. One castor germplasm is included in1, 2, 3 groups respectively, and two sub-groups are included in the 4 major group.

scholarly journals Assessment of Genetic Diversity and Population Structure of Some Soft and Hard Wheat Varieties Based on SSR Marker

2020 ◽  
Vol 8 (3) ◽  
pp. 80-87
Author(s):  
Yousif M. Fattah ◽  
Nergiz N. Tayib
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Triticum Aestivum L ◽  
Wheat Varieties ◽  
Discriminating Power ◽  
Triticum Spp ◽  
Cultivated Species ◽  
The Common ◽  
Diversity Studies

Wheat (Triticum spp.) is one of the most important cereal crops in Iraq and the world. It includes many species and varieties.  The two major cultivated species of wheat are, durum wheat (Tritium durum Desf.) which is tetraploid (2n= 28) and the common wheat (Triticum aestivum L.) which is hexaploid (2n = 42). Ten wheat varieties from both species were examined using ten Simple   sequence repeat (SSR) markers (WMC17, WMC20, WMC21, WMC24, WMC25, WMC48, WMC50, WMC283, Xgwm11 and Xgwm626). Various genetic parameters were calculated using Power Marker V3.25 software. A total of 156 alleles were detected in both species. The gene diversity in wheat varieties from both species collectively varied from 0.85 to 1.00, which indicates considerable genetic diversity in the examined varieties. All markers used in this study were highly informative and the polymorphic information content (PIC) values were higher than 0.50 in all loci. Hence all markers are considered useful for genetic diversity studies in wheat’s populations. The dendrogram separated the populations into two main clades and many subgroups. Azadi variety was simplicifolious. This study confirms the discriminating power of SSR typing and its usefulness for comparison within hard and soft wheat populations. 

scholarly journals Diversity of capsaicin content, quantitative, and yield components in chili (Capsicum annuum) genotypes and their F1 hybrid

2020 ◽  
Vol 21 (5) ◽  
Author(s):  
Zulfikar D Sahid ◽  
MUHAMAD SYUKUR ◽  
AWANG MAHARIJAYA
Keyword(s):  
Genetic Diversity ◽  
Capsicum Annuum ◽  
Block Design ◽  
Genetic Material ◽  
Morphological Characters ◽  
Fruit Weight ◽  
Yield Component ◽  
Quantitative Yield ◽  
F1 Hybrid ◽  
Randomized Complete Block Design

Abstract. Sahid ZD, Syukur M, Maharijaya A. 2020. Genetic diversity of capsaicin content, quantitative, and yield component in chili (Capsicum annuum) and their F1 hybrid. Biodiversitas 21: 2251-2257. Chili (Capsicum annuum L.) is one of the horticultural plants that have many benefits. The benefit of chili was determined by pungency level of its fruit. Pungency level of the chili is due to the capsaicin content in fruit. Information about the genetic diversity of capsaicin is still rarely available. The aims of this study were to obtain diversity information on quantitative, yield component, and capsaicin content, and to analyze the correlation among chili genotypes based on their morphological characters. This study used Randomized Complete Block Design with three replications. The genetic material used in this study consisted of 21 genotypes consisting of 6 genotypes of chili elders and 15 hybrid F1 genotypes resulting from their crossing. Six genotypes of the chili parents are C5, F6074, F9160291, Yuni, Bara, and Giant. 15 hybrid F1 genotypes used in this study are C5 x Bara, C5 x F6074, C5 x Yuni, C5 x Giant, C5 x F9160291, Bara x F6074, Bara x Yuni, Bara x Giant, Bara x F9160291, F6074 x Yuni, F6074 x Giant, F6074 x F9160291, Yuni x Giant, Yuni x F9160291, and Giant x F9160291. The observation was made on the variables of quantitative, yield, and capsaicin components on chili. The results showed that the highest capsaicin content only was found in Bara x F9160291. The results of scatterplot analysis showed that the highest capsaicin and yield component was found in BaraxF6074 and C5 x Yuni genotype. The results of cluster analysis showed that chili was clustered into three color groups. The character of capsaicin content is negatively correlated and very different from fruit weight, fruit diameter, fruit length, thick fruit flesh, total amount of fruit per plant, and fruit weight per plant.

scholarly journals Enrichment of chickpea genetic resources collection monitored by microsatellites

2009 ◽  
Vol 45 (No. 1) ◽  
pp. 11-17 ◽  
Author(s):  
K. Jomová ◽  
M. Benková ◽  
J. Kraic
Keyword(s):  
Genetic Diversity ◽  
Genetic Resources ◽  
Slovak Republic ◽  
Diversity Index ◽  
Polymorphic Information Content ◽  
Microsatellite Analysis ◽  
Entire Collection ◽  
Single Fragment ◽  
New Germplasm ◽  
New Alleles

A set of 49 chickpea accessions maintained in the collection of the Genebank of the Slovak Republic was analyzed using microsatellite markers. The level of genetic diversity and the effectivity of new germplasm acquisition for the collection enrichment were evaluated. Five primer pairs used generated 50 different amplified alleles. Four of them, TA2, TA5, TR1, and TR7, containing a long TAA-tandem repeat, were polymorphic with 11–13 alleles per locus. A single fragment was obtained from all the accessions with the primer pair CATPER flanking a small microsatellite repetition in the mRNA sequence of chickpea cationic peroxidase. The genetic diversity was expressed as a diversity index (DI) and polymorphic information content (PIC) with values in the range from 0.885 to 0.904 and from 0.972 to 0.991, respectively. Recently acquired new germplasm contributed 13 new alleles to the entire collection, i.e. almost 70% of microsatellite diversity originated from the recently acquired accessions. Remarkable is also, that one accession carries a unique allel, not present in the remaining collection. Microsatellite analysis revealed the importance of collecting missions for the enrichment of collections of genetic resources.

scholarly journals GENETIC DIVERSITY AMONG SWEET POTATO CROPS CULTIVATED BY TRADITIONAL FARMERS

2018 ◽  
Vol 31 (3) ◽  
pp. 779-790 ◽  
Author(s):  
PABLO FORLAN VARGAS ◽  
ERIC WATZKE ENGELKING ◽  
LUIS CARLOS FERREIRA DE ALMEIDA ◽  
ELIEL ALVES FERREIRA ◽  
HAMILTON CESAR DE OLIVERIA CHARLO
Keyword(s):  
Genetic Diversity ◽  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Block Design ◽  
Genetic Material ◽  
Morphological Characteristics ◽  
Leaf Size ◽  
Stem Length ◽  
Phenotypic Correlation ◽  
Crop Species

ABSTRACT The genetic variability within the crop species Ipomoea batatas is broad, hence, in order to support future breeding programs it is of the utmost importance that germplasm banks be created, conserved, and characterized. Therefore, the objective of this work was to rescue and evaluate the genetic divergence in sweet potato accessions collected in traditional communities of Vale do Ribeira Paulista. Sweet potato samples were collected from quilombos, indigenous villages, caiçaras communities, and small farms. The study was conducted between February 2013 and August 2014 in a randomized block design with three replications. Genetic material included 95 collected accessions and two commercial cultivars. Morphological characteristics of the accessions were evaluated and distances in the genetic distance matrix were estimated by means of multi-category variables, the data being subsequently clustered by the Tocher method. Analysis of the relative contribution of each characteristic and phenotypic correlation of descriptors was also performed. Results evidenced wide genetic diversity among the sweet potato accessions collected in Vale do Ribeira, which were not grouped according to the collection point. The descriptors that contributed more than 60% of genetic diversity included: leaf size, general leaf profile, immature leaf color, petiole pigmentation, predominant branch color, branch secondary color, stem length, cortical thickness, predominant periderm color, and periderm color intensity. Correlations between morphological descriptors was observed in 22.26% of the paired traits.

scholarly journals Lead uptake and tolerance of Ricinus communis L.

2006 ◽  
Vol 18 (4) ◽  
pp. 483-489 ◽  
Author(s):  
Solange Romeiro ◽  
Ana M.M.A. Lagôa ◽  
Pedro R. Furlani ◽  
Cleide A. de Abreu ◽  
Mônica F. de Abreu ◽  
...  
Keyword(s):  
Nutrient Solution ◽  
Ricinus Communis ◽  
Block Design ◽  
Physiological Responses ◽  
Tropical Species ◽  
Lead Uptake ◽  
Nutritive Solution ◽  
Ricinus Communis L ◽  
Light Concentration ◽  
Set Up

Phytoextraction is an important technique used for the decontamination of areas polluted by lead. Consequently, an understanding of the physiological responses to tolerance of tropical species subjected to increasing levels of contamination is fundamental before considering their use as phytoextractors in contaminated areas. The objective of this study was to assess the lead (Pb) uptake and the tolerance of Ricinus communis L. The plants were cultivated in nutrient solution in a greenhouse under controlled conditions. Lead was tested at concentrations of 0, 100, 200 and 400 µmol L-1. The experimental set-up was a block design, using a 4 x 1 factorial scheme, with three replicates. Biometric analyses, photosynthesis rates, and Pb content in the nutritive solution as well as in roots and shoots were performed. In conclusion, R. communis L is a hyperaccumulator species for Pb and presents tolerance properties in lead light concentration.

scholarly journals Molecular characterization of wheat (Triticum aestivum L.) genotypes through SSR markers

2012 ◽  
Vol 37 (3) ◽  
pp. 389-398 ◽  
Author(s):  
S Islam ◽  
MS Haque ◽  
RM Emon ◽  
MM Islam ◽  
SN Begum
Keyword(s):  
Genetic Diversity ◽  
Triticum Aestivum ◽  
Genetic Distance ◽  
Microsatellite Markers ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Allelic Diversity ◽  
Triticum Aestivum L ◽  
Allele Number ◽  
Average Gene

A study was undertaken to examine the genetic diversity of 12 wheat (Triticum aestivum L.) genotypes, using 4 simple sequence repeats (SSRs). A total of 10 alleles were found. Allele number per locus ranged from 2 to 4 with an average of 2.5. The polymorphic information content (PIC) values ranged from 0.2755 to 0.5411 with an average of 0.3839. The average gene diversity over all SSR loci for the 12 wheat genotypes was 0.4688, ranging from 0.3299 to 0.6042. Cluster analysis based on microsatellite allelic diversity discriminated the varieties into different clusters. Genetic diversity was the highest between variety Gourab and Akbar as well as Gourab and BAW-1064, showing a genetic distance value of 0.4697. The genetic distance was lowest between Balaka and Aghrani as well as Triticale and BAW-1036. Positive correlations were found between gene diversity, number of alleles, the allele size range and the types of repeat motif of microsatellite markers. It was found from this study that microsatellite markers could characterize and discriminate all of the genotypes. More primers should be used for saturation of different regions in further studies. Bangladesh J. Agril. Res. 37(3): 389-398, September 2012 DOI: http://dx.doi.org/10.3329/bjar.v37i3.12082

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